Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis

Liu, Lukuan; Yang, Kaiguang; Zhu, Xudong; Liang, Yu; Chen, Yuanbo; Fang, Fei; Zhao, Qun; Zhang, Lihua; Zhang, Yukui

doi:10.1016/j.talanta.2017.07.041

Cited by 15 publications

(6 citation statements)

References 23 publications

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“…106,107 However, performing liquid chromatography via open tubular columns is challenging primarily because of their disadvantages of very low sample loadability. To obtain an open-tubular column with a relatively enhanced sample loadability, some methods have been developed, such as coating with porous layers, 108–113 and depositing of nanoparticles 114–116 on the inner wall of the capillaries. Among them, Karger et al pioneered the preparation of poly(styrene-divinylbenzene) (PS-DVB) layers in a 4.2 m × 10 μm i.d.…”

Section: The Development Of Separation Columnsmentioning

confidence: 99%

“…To obtain an open-tubular column with a relatively enhanced sample loadability, some methods have been developed, such as coating with porous layers, [108][109][110][111][112][113] and depositing of nanoparticles [114][115][116] on the inner wall of the capillaries. Among them, Karger et al pioneered the preparation of poly-(styrene-divinylbenzene) (PS-DVB) layers in a 4.2 m Â 10 mm i.d.…”

Section: Open-tubular Columnsmentioning

confidence: 99%

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Chromatographic separation of peptides and proteins for characterization of proteomes

Liang

Zhang

2023

Chem. Commun.

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Section: The Development Of Separation Columnsmentioning

confidence: 99%

Section: Open-tubular Columnsmentioning

confidence: 99%

Chromatographic separation of peptides and proteins for characterization of proteomes

Liang

Zhang

2023

Chem. Commun.

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“…The association equilibrium constants for binding by these proteins with biotin range from 10 13 to 10 15 M –1 [247,248]. Biotinylated aptamers have been coupled to monolithic supports containing avidin or streptavidin [249–252]. Monomeric avidin has also been placed in organic monoliths to enrich biotinylated cytochrome C and its corresponding peptides [253].…”

Section: Applications Of Amcmentioning

confidence: 99%

Affinity monolith chromatography: A review of general principles and recent developments

2021

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Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a monolithic support with a biological ligand or related binding agent to isolate, enrich, or detect a target analyte in a complex matrix. The target‐specific interaction exhibited by the binding agents makes AMC attractive for the separation or detection of a wide range of compounds. This article will review the basic principles of AMC and recent developments in this field. The supports used in AMC will be discussed, including organic, inorganic, hybrid, carbohydrate, and cryogel monoliths. Schemes for attaching binding agents to these monoliths will be examined as well, such as covalent immobilization, biospecific adsorption, entrapment, molecular imprinting, and coordination methods. An overview will then be given of binding agents that have recently been used in AMC, along with their applications. These applications will include bioaffinity chromatography, immunoaffinity chromatography, immobilized metal‐ion affinity chromatography, and dye‐ligand or biomimetic affinity chromatography. The use of AMC in chiral separations and biointeraction studies will also be discussed.

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“…The very high binding constant between biotin and streptavidin in the order of K d = 10 −15 mol/L enables essentially irreversible immobilization of biotinylated ligands on streptavidin–GNP conjugates. This approach is often used for the immobilization of biotinylated nucleic acids , antibodies, and aptamers (e.g., directed against circulating tumor cells ).…”

Section: Synthesis and Surface Modificationmentioning

confidence: 99%

Functionalized gold nanoparticles for sample preparation: A review

Liu

Lämmerhofer

2019

Electrophoresis

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Sample preparation is a crucial step for the reliable and accurate analysis of both small molecule and biopolymers which often involves processes such as isolation, pre‐concentration, removal of interferences (purification), and pre‐processing (e.g., enzymatic digestion) of targets from a complex matrix. Gold nanoparticle (GNP)‐assisted sample preparation and pre‐concentration has been extensively applied in many analytical procedures in recent years due to the favorable and unique properties of GNPs such as size‐controlled synthesis, large surface‐to‐volume ratio, surface inertness, straightforward surface modification, easy separation requiring minimal manipulation of samples. This review article primarily focuses on applications of GNPs in sample preparation, in particular for bioaffinity capture and biocatalysis. In addition, their most common synthesis, surface modification and characterization methods are briefly summarized. Proper surface modification for GNPs designed in accordance to their target application directly influence their functionalities, e.g., extraction efficiencies, and catalytic efficiencies. Characterization of GNPs after synthesis and modification is worthwhile for monitoring and controlling the fabrication process to ensure proper quality and functionality. Parameters such as morphology, colloidal stability, and physical/chemical properties can be assessed by methods such as surface plasmon resonance, dynamic light scattering, ζ‐potential determinations, transmission electron microscopy, Taylor dispersion analysis, and resonant mass measurement, among others. The accurate determination of the surface coverage appears to be also mandatory for the quality control of functionality of the nanoparticles. Some promising applications of (functionalized) GNPs for bioanalysis and sample preparation are described herein.

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Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis

Cited by 15 publications

References 23 publications

Chromatographic separation of peptides and proteins for characterization of proteomes

Chromatographic separation of peptides and proteins for characterization of proteomes

Affinity monolith chromatography: A review of general principles and recent developments

Functionalized gold nanoparticles for sample preparation: A review

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