Aptamer-functionalized silver nanoparticles for scanometric detection of platelet-derived growth factor-BB

Hu, Hongting; Li, Hui; Zhao, Yaju; Dong, Sining; Li, Wei; Qiang, Weibing; Xu, Danke

doi:10.1016/j.aca.2013.12.026

Cited by 20 publications

(13 citation statements)

References 50 publications

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“…The detection limit was 0.125 nM. The sensitivity was compared with that from recently reported aptamer based assays for PDGF-BB (shown in Table 1) [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35]. The sensitivity of our assay is comparable to that reported in some methods [21-27, 32, 34].…”

Section: Resultssupporting

confidence: 64%

Determination of the platelet-derived growth factor BB by a competitive thrombin-linked aptamer-based Fluorometric assay

Guo

Zhao

2016

Microchim Acta

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The authors describe a competitive aptamer based assay for detection of the platelet-derived growth factor BB (PDGF-BB; used as a model protein). The assay is making use of thrombin (a serine protease) as an enzyme label for reporting signals. It is taking advantage of a highly selective aptamer and of the fairly specific enzymatic activity of thrombin in terms of cleaving artificial fluorogenic peptide substrates. In a first step, the surface of wells of microplates is coated with PDGF-BB. On addition of a sample containing PDGF-BB, free and bound PDGF-BB compete with each other for binding to a DNA probe that consists of an aptamer sequence for PDGF-BB and a 29-mer aptamer sequence for thrombin. After washing, thrombin is added and will attach to the DNA probe that bound to the PDGF-BB on the microplates. Following addition of a fluorogenic peptide substrate, the bound thrombin will catalyze the cleavage of the substrate to generate a fluorescent product whose fluorescence intensity is measured at excitation/emission wavelengths of 370/ 440 nm. Fluorescence intensity decreases with increasing PDGF-BB concentration in the sample because less thrombin will bind to the PDGF-BB coated surface of the microplate. Under optimal conditions, PDGF-BB can be quantified in the 0.125 to 3 nM concentration range. This assay was successfully applied to the determination of PDGF-BB in spiked 100fold diluted human serum.

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Section: Resultssupporting

confidence: 64%

Determination of the platelet-derived growth factor BB by a competitive thrombin-linked aptamer-based Fluorometric assay

Guo

Zhao

2016

Microchim Acta

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“…Ten to hundred fold improvements over conventional methods was seen with the method. The approach promises high sensitivity, simple design, and convenient operation [ 29 ].…”

Section: Biomedical Applicationsmentioning

confidence: 99%

“…The results demonstrated that color agent 1 had benefits over the other option, especially at low detection limits due to its superior sensitivity. The development showed good potential in complex biological samples [ 29 ].…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Biomedical Probes Based on Inorganic Nanoparticles for Electrochemical and Optical Spectroscopy Applications

Yakoh

Pinyorospathum

Siangproh

et al. 2015

Sensors

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Inorganic nanoparticles usually provide novel and unique physical properties as their size approaches nanometer scale dimensions. The unique physical and optical properties of nanoparticles may lead to applications in a variety of areas, including biomedical detection. Therefore, current research is now increasingly focused on the use of the high surface-to-volume ratios of nanoparticles to fabricate superb chemical- or biosensors for various detection applications. This article highlights various kinds of inorganic nanoparticles, including metal nanoparticles, magnetic nanoparticles, nanocomposites, and semiconductor nanoparticles that can be perceived as useful materials for biomedical probes and points to the outstanding results arising from their use in such probes. The progress in the use of inorganic nanoparticle-based electrochemical, colorimetric and spectrophotometric detection in recent applications, especially bioanalysis, and the main functions of inorganic nanoparticles in detection are reviewed. The article begins with a conceptual discussion of nanoparticles according to types, followed by numerous applications to analytes including biomolecules, disease markers, and pharmaceutical substances. Most of the references cited herein, dating from 2010 to 2015, generally mention one or more of the following characteristics: a low detection limit, good signal amplification and simultaneous detection capabilities.

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“…However, many targets, such as thrombin [28,29], platelet-derived growth factor [30,31], prostate specific antigen [32], and vascular endothelial growth factors [33] and MUC1 protein [34], have more than one sites to bind their aptamers or antibodies to form a sandwich structure, which has an obviously better anti-interference ability than the simple biosensing structure [35]. Although lots of E-AB biosensors have been developed to detect these targets based on single-signaling strategy, so far no dual-signaling E-AB biosensors have utilized the aptamer-target-aptamer sandwich structure with both aptamers labeled by redox species for robust detection of the target.…”

Section: Introductionmentioning

confidence: 99%

A ratiometric electrochemical aptasensor for sensitive detection of protein based on aptamer–target–aptamer sandwich structure

Zhou

et al. 2014

Journal of Electroanalytical Chemistry

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Aptamer-functionalized silver nanoparticles for scanometric detection of platelet-derived growth factor-BB

Cited by 20 publications

References 50 publications

Determination of the platelet-derived growth factor BB by a competitive thrombin-linked aptamer-based Fluorometric assay

Determination of the platelet-derived growth factor BB by a competitive thrombin-linked aptamer-based Fluorometric assay

Biomedical Probes Based on Inorganic Nanoparticles for Electrochemical and Optical Spectroscopy Applications

A ratiometric electrochemical aptasensor for sensitive detection of protein based on aptamer–target–aptamer sandwich structure

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