Aptamer-functionalized hydrogel as effective anti-cancer drugs delivery agents

Wang, Zonghua; Xia, Jianfei; Cai, Feng; Zhang, Feifei; Yang, Min; Bi, Sai; Gui, Rijun; Li, Yanhui; Xia, Yanzhi

doi:10.1016/j.colsurfb.2015.06.031

Cited by 29 publications

(18 citation statements)

References 31 publications

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“…DNA thus offers remarkable sensitivity and specificity in its interactions and the hydrogel provides a means to amplify the molecular signal to a microscopic length scale. The possible applications are in sensing [4,10,14,15], targeted drug delivery [16][17][18], as well as in tissue engineering and as soft devices [1,[19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels

Jonášová

Stokke

2020

Polymers

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Responsive hydrogels featuring DNA as a functional unit are attracting increasing interest due to combination of versatility and numerous applications. The possibility to use nucleic acid analogues opens for further customization of the hydrogels. In the present work, the commonly employed DNA oligonucleotides in DNA-co-acrylamide responsive hydrogels are replaced by Morpholino oligonucleotides. The uncharged backbone of this nucleic acid analogue makes it less susceptible to possible enzymatic degradation. In this work we address fundamental issues related to key processes in the hydrogel response; such as partitioning of the free oligonucleotides and the strand displacement process. The hydrogels were prepared at the end of optical fibers for interferometric size monitoring and imaged using confocal laser scanning microscopy of the fluorescently labeled free oligonucleotides to observe their apparent diffusion and accumulation within the hydrogels. Morpholino-based hydrogels’ response to Morpholino targets was compared to DNA hydrogels’ response to DNA targets of the same base-pair sequence. Non-binding targets were observed to be less depleted in Morpholino hydrogels than in DNA hydrogels, due to their electroneutrality, resulting in faster kinetics for Morpholinos. The electroneutrality, however, also led to the total swelling response of the Morpholino hydrogels being smaller than that of DNA, since their lack of charges eliminates swelling resulting from the influx of counter-ions upon oligonucleotide binding. We have shown that employing nucleic acid analogues instead of DNA in hydrogels has a profound effect on the hydrogel response.

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Section: Introductionmentioning

confidence: 99%

Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels

Jonášová

Stokke

2020

Polymers

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“…Hence, aptamers were usually incorporated into hydrogels as affinity ligands to expand the application of hydrogels in the field of controlled drug delivery [24][25][26]. For instance, a sustainedrelease polyacrylamide (PAA) hydrogel system was achieved through using platelet-derived growth factor-BB aptamer to reserve the protein drugs [20].…”

Section: Introductionmentioning

confidence: 99%

“…For instance, a sustainedrelease polyacrylamide (PAA) hydrogel system was achieved through using platelet-derived growth factor-BB aptamer to reserve the protein drugs [20]. As reported in recent literatures, most aptamers were usually functionalized into synthetic polymeric hydrogels such as PAA or polyethylene glycol methacrylate (PEGMA) by radical polymerization [24,[27][28][29]. The strict demands of the modified process for aptamer−polymer hybrids and the toxicity of all kinds of initiators limit their application in vivo in the biomedical fields.…”

Section: Introductionmentioning

confidence: 99%

Aptamer Functionalized DNA Hydrogel for Wise-Stage Controlled Protein Release

et al. 2018

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With the simple functionalization method and good biocompatibility, an aptamer-integrated DNA hydrogel is used as the protein delivery system with an adjustable release rate and time by using complementary sequences (CSs) as the biomolecular trigger. The aptamer-functionalized DNA hydrogel was prepared via a one-pot self-assembly process from two kinds of DNA building blocks (X-shaped and L-shaped DNA units) and a single-stranded aptamer. The gelling process was achieved under physiological conditions within one minute. In the absence of the triggering CSs, the aptamer grafted in the hydrogel exhibited a stable state for protein-specific capture. While hybridizing with the triggering CSs, the aptamer is turned into a double-stranded structure, resulting in the fast dissociation of protein with a wise-stage controlled release program. Further, the DNA hydrogel with excellent cytocompatibility has been successfully applied to human serum, forming a complex matrix. The whole process of protein capture and release were biocompatible and could not refer to any adverse factor of the protein or cells. Thus, the aptamer-functionalized DNA hydrogel will be a good candidate for controlled protein delivery.

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“…Zonghua Wang et al [20] demonstrated the general design of a drug delivery system based on an aptamer cross-linked hydrogel. The AS1411 is a promoter which leads the hybridization of acrydite-modified oligonucleotides to form the hydrogels.…”

Section: Aptamer-hydrogel Conjugatesmentioning

confidence: 99%

“…The past few years has witnessed the great advances in the synthesis and characterization of various nanomaterials, such as dots, [17,18] gold nanomaterials, [19] hydrogel [20] carbon nanotubes [21] liposome [22] and so on. The combination of aptamers with novel nanomaterials has led to a wide applications.…”

Section: Aptamer-nanoparticles Systemsmentioning

confidence: 99%

Applications of Aptamers in Drug Delivery System

Wu¹

2017

WJPR

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Aptamers, a kind of short single-stranded oligonucleic acids (DNA or RNA) or peptide molecules that can fold into secondary or tertiary structures, are well recognized as a potential tool for binding target molecules due to its high specificity and affinity. As tumor targeted ligands, aptamers have certain superiorities compared with antibodies, such as low immunogenicity, high binding specificity and so on. By the means of systematic evolution of ligands with exponential enrichment (SELEX) approach, selected aptamers showed high affinity to specific targets like proteins, peptides, and even the entire cells. In this review, we will give a brief overview of recent relevant research in the field of aptamers and their applications in cancer diagnostics and in drug delivery system.

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Aptamer-functionalized hydrogel as effective anti-cancer drugs delivery agents

Cited by 29 publications

References 31 publications

Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels

Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels

Aptamer Functionalized DNA Hydrogel for Wise-Stage Controlled Protein Release

Applications of Aptamers in Drug Delivery System

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