Aptamer-functionalized Fe₃O₄magnetic nanoparticles as a solid-phase extraction adsorbent for the selective extraction of berberine fromCortex phellodendri

Abstract

The extraction adsorbent was fabricated by immobilizing the highly specific recognition and binding of aptamer onto the surface of Fe O magnetic nanoparticles, which not only acted as recognition elements to recognize and capture the target molecule berberine from the extract of Cortex phellodendri, but also could favor the rapid separation and purification of the bound berberine by using an external magnet. The developed solid-phase extraction method in this work was useful for the selective extraction and determination of berberine in Cortex phellodendri extracts. Various conditions such as the amount of aptamer-functionalized Fe O magnetic nanoparticles, extraction time, temperature, pH value, Mg concentration, elution time and solvent were optimized for the solid-phase extraction of berberine. Under optimal conditions, the purity of berberine extracted from Cortex phellodendri was as high as 98.7% compared with that of 4.85% in the extract, indicating that aptamer-functionalized Fe O magnetic nanoparticles-based solid-phase extraction method was very effective for berberine enrichment and separation from a complex herb extract. The applicability and reliability of the developed solid-phase extraction method were demonstrated by separating berberine from nine different concentrations of one Cortex phellodendri extract. The relative recoveries of the spiked solutions of all the samples were between 95.4 and 111.3%, with relative standard deviations ranging between 0.57 and 1.85%.

Determination of sulfadimethoxine in milk with aptamer‐functionalized Fe3O4/graphene oxide as magnetic solid‐phase extraction adsorbent prior to HPLC

“…Evidently, the correlation coefficient of Freundlich isotherm model (0.9878, 0.9991, and 0.9938) are higher than that of Langmuir isotherm model (0.8915, 0.8384, and 0.8982), indicating that the adsorption process can be better described by the Freundlich isothermal model, and the adsorption of SDM on Fe 3 O 4 /GO/Apt is multilayered and uneven process. In addition, the adsorbent exhibits higher adsorption capacity at 298 K, indicating higher temperature is unfavorable for Fe 3 O 4 /GO/Apt to capture the analyte, which is also consistent with the previous report [21,53].…”

Extraction in cholinium-based magnetic ionic liquid aqueous two-phase system for the determination of berberine hydrochloride in Rhizoma coptidis

“…A good linear relationship indicates the acceptable consistency of the experimental results. The values of these tting parameters of eqn (10) and (11) with the corresponding correlation coefficient values (R 2 ) are listed in Table S4. † The tie-lines for ve MILs (1) + K 3 PO 4 (2) + water (3) systems with the same phase composition are compared and illustrated in Fig.…”

“…The tie-line compositions, including tie-line length (TLL) and the slope of the tie-line (S) are listed in Table S3. † The values of these tting parameters of eqn (10) and (11) with the corresponding correlation coefficient values (R 2 ) are listed in Table S4. † 3.1.3 The effect of temperature on binodal curves.…”

“…7,8 Moreover, some functional nanoparticles have been used for the selective extraction of berberine in recent years. 9,10 However, these extraction techniques have some limitations and disadvantages such as high cost/ consumption, low extraction efficiency, or long extraction time. Therefore, more and more researchers are focusing on improving and optimizing the extraction and separation techniques for the extraction of active components from natural products.…”

Magnetic separation coupled with high‐performance liquid chromatography–mass spectrometry for rapid separation and determination of lignans in Schisandra chinensis

“…In recent years, magnetic nanoparticles (MNPs) have attracted a considerable attention because of its larger specific surface area [14], high saturation magnetic moment and superparamagnetism, easy functionality, and convenient manipulation [15], which provide a broad application potential in biology, medicine, separation, and other fields [16][17][18][19][20][21][22][23]. Magnetic separation has several advantages compared to the traditional separation methods.…”