Aptamer-Decorated Self-Assembled Aggregation-Induced Emission Organic Dots for Cancer Cell Targeting and Imaging

Zhang, Pengfei; Zhao, Zheng; Li, Chuansheng; Su, Huifang; Wu, Yayun; Kwok, Ryan T. K.; Lam, Jacky W. Y.; Gong, Ping; Cai, Lintao; Tang, Ben Zhong

doi:10.1021/acs.analchem.7b03933

Cited by 69 publications

(37 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to the above‐mentioned aptamer–nanomaterial systems, other types of aptamer‐based nanomaterials, such as liposome, silica nanomaterials, polymer nanomaterials, MOFs, dendrimers, micellar nanoparticles, gadolinium oxide nanoparticles, calcium carbonate nanostructure, polydopamine nanospheres, MoS 2 nanoplates, lipid‐based nanobubbles, dipeptide nanoparticles, aggregation‐induced emission organic dots, and MnO 2 nanosheet, have been engineered as promising candidates toward in vivo applications in diverse areas. Based on these studies, we summarize the representative reports focusing on aptamers integrated with other nanomaterials, as shown in Table 2 .…”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

View full text Add to dashboard Cite

equally exciting and perhaps more challenging field of application is toward their in vivo applications, including living animals and human bodies, in diverse areas, [2] such as sensing, drug delivery, medical imaging, and cancer therapy. However, most of these nanomaterials lack selectivity toward targets of interests. Therefore, to employ these nanomaterials for a wider range of in vivo applications, various molecular engineering approaches have been employed to obtained nanomaterials functionalized with biomolecules to enable selective targeting. [3][4][5] Among these biomolecules, functional nucleic acids, or FNAs, have shown the most promise.FNAs are DNA or RNA molecules that can interact with or bind to a specific analyte, resulting in a conformational change and/or a catalytic reaction. [6] As their names suggested, ribozymes and deoxyribozymes (called DNAzyme hereafter) can act as enzymes in the presence of cofactors to catalyze various reactions, including cleavage and ligation of RNA/DNA, and phosphorylation and peroxidation of DNA. On the other hand, riboswitches and DNA aptamers are antibody-like receptors that can bind target molecules with high affinity and selectivity. Furthermore, the binding abilities of riboswitches and aptamers can be combined with the enzymatic function of ribozymes or DNAzymes to form aptazymes so that the binding of targets can inhibit or enhance the catalytic activities. These nucleic acids, including DNAzymes, aptamers, and aptazymes, are collectively known as FNAs to emphasize their functions beyond the traditional genetic storage and transformations roles for DNA and RNA. Unlike DNA and RNA inside biological systems, FNAs are isolated via a combinatorial technique known as in vitro selection, or a process also known as systematic evolution of ligands by exponential enrichment (SELEX). The discovery of new functions of nucleic acids has not only resulted in major advances in the fields of molecular biology and biochemistry, but also revolutionized sensors designs for both in vitro and in vivo applications. [7,8] Over the past decade, numerous FNA-based nanomaterials have been developed. [9][10][11][12] Among these FNA-based nanosystems, the functions of FNAs can be categorized into two types: diagnostic function and therapeutic function. For the diagnostic function, the FNAs serve either as the biorecognition ligands for direct sensing and imaging of the Recent advances in nanotechnology and engineering have generated many nanomaterials with unique physical and chemical properties. Over the past decade, numerous nanomaterials are introduced into many research areas, such as sensors for environmental monitoring, food safety, point-ofcare diagnostics, and as transducers for solar energy transfer. Meanwhile, functional nucleic acids (FNAs), including nucleic acid enzymes, aptamers, and aptazymes, have attracted major attention from the biomedical community due to their unique target recognition and catalytic properties. Benefiting from the recent progress of molecular engine...

show abstract

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…Considering its excellent cellular permeability, low cytotoxicity, and biocompatibility, TPE‐aptamer might enable the uncovering of the other biological process for disease diagnosis. In 2018, Tang and co‐workers developed aptamer‐functionalized TPE Apt‐AIE dots with fabricating lipid‐decorated self‐assembly, as shown in Figure B. The Apt‐AIE integrates the advantages of the aggregates of hydrophobic AIEgens and the specific targeting capability of aptamers.…”

Section: Key Application In Biomedicinementioning

confidence: 99%

“…B) The composite structure of aptamer‐functionalized TPE Apt‐AIE dots and its fluorescence imaging for cancer MCF cells against normal 293T cells. Adapted with permission . Copyright 2018, American Chemical Society.…”

Section: Key Application In Biomedicinementioning

confidence: 99%

Biomacromolecule‐Functionalized AIEgens for Advanced Biomedical Studies

Duan

et al. 2019

Small

View full text Add to dashboard Cite

The advances in bioinformatics and biomedicine have promoted the development of biomedical imaging and theranostic systems to respectively extend the endogenous biomarker imaging with high contrast and enhance the therapeutic effect with high efficiency. The emergence of biomacromolecule‐functionalized aggregation‐induced emitters (AIEgens), utilizing AIEgens, and biomacromolecules (nucleic acids, peptides, glycans, and lipids), displays specific targeting ability to cancer cell, improved biocompatibility, reduced toxicity, enhanced therapeutic effect, and so forth. This review summarizes the rational design of biomacromolecule‐functionalized AIEgens and their biomedical applications in recent ten years, including high‐resolution optical imaging of cell, tissue, and small animal model with low background; the biomarker detection for early diagnosis and prognosis; the delivery and monitoring of prodrugs; image‐guide photodynamic therapy and its combination with chemotherapy. Through illustrating their functional mechanisms and application, it is hoped that this review would open up a completely new train of research thought for attracted researchers in various fields.

show abstract

“…Av ariety of aptamer-functionalized and AIE-embeddedD NA probes( Apt-AIE sensors) have been developed through ag eneral approach, and thesel abel-freef luorescent aptasensors were applied for specific and supersensitive monitoring of in-tracellularA TP, [23] ochratoxin A( OTA), [24] prostate-specific antigen (PSA), [25] targeted DNA and thrombin protein, [26] Hg 2 + and GSH, [27] and targeted cell imaging of MCF-7 cells. [28] Ta ng andc o-workers [29] demonstrated af acile one-step assay to construct aptamer-anchoreds elf-assembled organic dots with AIE properties. As shown in Figure 2, the Apt-AIEd ots were fabricated through mixingo ft he aptamer-cholesterol, lipids, polyethylene glycol (PEG)-lipids, and AIEgensunder sonication.…”

Section: Constant Dna Lengthmentioning

confidence: 99%

AIEgens/Nucleic Acid Nanostructures for Bioanalytical Applications

Wang

Huang

et al. 2019

Chemistry An Asian Journal

View full text Add to dashboard Cite

DNA occupies significant roles in life processes, which include encoding the sequences of proteins and accurately transferring genetic information from generation to generation. Recent discoveries have demonstrated that a variety of biological functions are correlated with DNA′s conformational transitions. The non‐B form has attained great attention among the diverse forms of DNA over the past several years. The main reason for this is that a large number of studies have shown that the non‐B form of DNA is associated with gross deletions, inversions, duplications, translocations as well as simple repeating sequences, which therefore causes human diseases. Consequently, the conformational transition of DNA between the B‐form and the non‐B form is important for biology. Conventional fluorescence probes based on the conformational transitions of DNA usually need a fluorophore and a quencher group, which suffers from the complex design of the structure and tedious synthetic procedures. Moreover, conventional fluorescence probes are subject to the aggregation‐caused quenching (ACQ) effect, which limits their application toward imaging and analyte detection. Fluorogens exhibiting aggregation‐induced emission (AIE) have attracted tremendous attention over the past decade. By taking advantage of this unique behavior, plenty of fluorescent switch‐on probes without the incorporation of fluorescent quenchers/fluorophore pairs have been widely developed as biosensors for imaging a variety of analytes. Herein, the recent progress in bioanalytical applications on the basis of aggregation‐induced emission luminogens (AIEgens)/nucleic acid nanostructures are presented and discussed.

show abstract

Aptamer-Decorated Self-Assembled Aggregation-Induced Emission Organic Dots for Cancer Cell Targeting and Imaging

Cited by 69 publications

References 38 publications

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Biomacromolecule‐Functionalized AIEgens for Advanced Biomedical Studies

AIEgens/Nucleic Acid Nanostructures for Bioanalytical Applications

Contact Info

Product

Resources

About