Aptamer decorated magnetic graphene oxide nanoparticles for effective capture of exosomes

Huang, Weiguo; Yu, Yunru; Yang, Chaoyu; zhang, Xiaohui; Shang, Luoran; Zu, Yan; Shi, Ke‐Qing

doi:10.1016/j.cej.2021.133849

Cited by 26 publications

(10 citation statements)

References 36 publications

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“…Briefly, Fe 3 O 4 NPs (termed NP‐1 hereafter) were synthesized using the solvothermal methods as previously described. [ 20 ] Then, polyethyleneimine (PEI) was modified on the surface to obtain Fe 3 O 4 @PEI (termed NP‐2 hereafter) after incubation with shaking. [ 21 ] Subsequently, NP‐2 was decorated with DSP to produce the Fe 3 O 4 @PEI‐DSP (termed NP‐3 hereafter).…”

Section: Resultsmentioning

confidence: 99%

Cell Membranes from Tumor‐Tropic MSCs Screened by a Microfluidic Chip for Drug Nanoparticles Encapsulation and Cancer Targeted Therapy

Song

Shou

Sheng

et al. 2023

Adv Healthcare Materials

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Nanoparticles (NPs)-based drug carriers are effective in reducing systemic toxicity and drug resistance for chemotherapy, and an emerging trend focuses on integrating cell membranes with nanomedicines for tumor targeting. Mesenchymal stem cells (MSCs) are promising candidates due to their unique tropism toward cancer cells, yet the tumor-tropic abilities can differ for MSCs sourced from different tissues. Here, a multichannel microfluidic chip to screen different sourced MSCs with the greatest tropism toward cervical cancer cells is developed. Based on this, the cell membranes from the chorionic plate-derived MSC are isolated and membrane-camouflaged platinum prodrug composite NPs for cervical cancer treatment are prepared. Results demonstrate that the composite NPs can effectively target tumor sites and have a therapeutic effect both in vitro and in vivo. It is believed that the present microfluidic platform is a powerful tool for cell screening and tumor-on-a-chip studies, and the derived nanodelivery system represents the great value of cell membrane-camouflaged nanomedicine for targeted cancer therapy.

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Section: Resultsmentioning

confidence: 99%

Cell Membranes from Tumor‐Tropic MSCs Screened by a Microfluidic Chip for Drug Nanoparticles Encapsulation and Cancer Targeted Therapy

Song

Shou

Sheng

et al. 2023

Adv Healthcare Materials

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“…Utilizing the abundant biomarkers on the membranes of EVs, affinitybased EV isolation/capture approaches have been proposed using antibodies or aptamers as recognition ligands with high specificity and efficiency. The ease of chemical modification of aptamers enables them to be modified on different interfaces for EV isolation, including Au electrodes, 11,79 MoS 2 -Fe 3 O 4 nanostructures (MOFE), 13 graphene oxide (GO) decorated Fe 3 O 4 @SiO 2 magnetic nanoparticles (NPs), 14 Fe 3 O 4 @TiO 2 NPs, 18 and magnetic beads. 78,103 Instead of modifying aptamers directly on magnetic beads for EV capture, Zhang's group marked EVs with biotin-labeled CD63 aptamers and separated EVs using Streptavidin-modified magnetic beads via Streptavidinbiotin interaction (Figure 2A).…”

Section: Methods For Evsmentioning

confidence: 99%

Aptamer‐based extracellular vesicle isolation, analysis and therapeutics

Zhu

Tian

Yuan³

et al. 2023

Interdisciplinary Medicine

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Extracellular vesicles (EVs) play an important role in many physiological processes. Thus, EV analysis has a great value for the understanding of mechanisms underlying disease progress or diagnosis, prognosis and therapy. The overlapped physical and immune properties between EVs and events in body fluids, as well as the phenotypic heterogeneity of EVs, require efficient isolation and analysis methods. The unique properties of aptamers, such as facile modification and programmability, make them easily assembled as powerful platforms for EV isolation and analysis. EVs can also be used as vehicles for drug delivery, benefiting from the properties of homing ability, hypo-immunogenicity, and strong tolerance. The affinity recognition ability to targets and the feature of single stranded DNA of aptamers make them useful in promoting the targetability of EVs and delivery of nucleic acid drugs. This review summarizes recent progress in aptamer-based EV isolation, analysis, and aptamer-functionalized EVs for therapeutics. K E Y W O R D Saptamer, drug delivery, extracellular vesicles, extracellular vesicle detection, extracellular vesicle isolation | INTRODUCTIONExtracellular vesicles (EVs) are phospholipid bilayerenclosed extracellular structures present in body fluids, such as blood, urine, saliva, and tears. 1,2 EVs are formed by outward budding of cell membranes (ectosomes) or the intracellular endocytic trafficking pathway involving the fusion of late endosomal multivesicular bodies with the cell membrane (exosomes). 3,4 Ectosomes, including microparticles, micro-vesicles, and large vesicles, are released from the plasma membrane with a size range of 50 nm to 1 μm and were found to be messengers to transfer functional proteins and nucleic acids. [5][6][7] In addition, exosomes are small vesicles secreted from all types of cells This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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“…This label-free technique can directly quantify exosomes in serum samples and has shown diagnostic potential for prostate cancer. Additionally, the porous structure has been used to isolate exosomes with and without other isolation materials and devices [ 191 , 192 , 193 ].…”

Section: Carbon Nanomaterialsmentioning

confidence: 99%

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

et al. 2023

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Extracellular vesicles (EVs) have emerged as a novel resource of biomarkers for cancer and certain other diseases. Probing EVs in body fluids has become of major interest in the past decade in the development of a new-generation liquid biopsy for cancer diagnosis and monitoring. However, sensitive and specific molecular detection and analysis are challenging, due to the small size of EVs, low amount of antigens on individual EVs, and the complex biofluid matrix. Nanomaterials have been widely used in the technological development of protein and nucleic acid-based EV detection and analysis, owing to the unique structure and functional properties of materials at the nanometer scale. In this review, we summarize various nanomaterial-based analytical technologies for molecular EV detection and analysis. We discuss these technologies based on the major types of nanomaterials, including plasmonic, fluorescent, magnetic, organic, carbon-based, and certain other nanostructures. For each type of nanomaterial, functional properties are briefly described, followed by the applications of the nanomaterials for EV biomarker detection, profiling, and analysis in terms of detection mechanisms.

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Aptamer decorated magnetic graphene oxide nanoparticles for effective capture of exosomes

Cited by 26 publications

References 36 publications

Cell Membranes from Tumor‐Tropic MSCs Screened by a Microfluidic Chip for Drug Nanoparticles Encapsulation and Cancer Targeted Therapy

Cell Membranes from Tumor‐Tropic MSCs Screened by a Microfluidic Chip for Drug Nanoparticles Encapsulation and Cancer Targeted Therapy

Aptamer‐based extracellular vesicle isolation, analysis and therapeutics

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

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