Aptamer‐Based Turn‐On Detection of Thrombin in Biological Fluids Based on Efficient Phosphorescence Energy Transfer from Mn‐Doped ZnS Quantum Dots to Carbon Nanodots

Zhang, Lu; Cui, Peng; Zhang, Baocheng; Gao, Feng

doi:10.1002/chem.201300588

Cited by 61 publications

(40 citation statements)

References 105 publications

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“…‘Activatable’ probes can be superior to ‘always on’ probes since they emit much less fluorescence in the absence of a target, thereby increasing the signal-to-background ratio (26,27). Our experiments with fluorescence spectroscopy revealed that AAFP emitted minimal fluorescence in the absence of HepG2 cells, likely reflecting fluorescence resonance energy transfer between the fluorophore and quencher lying close together in the hairpin structure formed upon hybridization of the two C-strands.…”

Section: Discussionmentioning

confidence: 99%

An ‘activatable’ aptamer-based fluorescence probe for the detection of HepG2 cells

et al. 2017

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It is significant to develop a probe with sensitivity and specificity for the detection of cancer cells. The present study aimed to develop an ‘activatable’ aptamer-based fluorescence probe (AAFP) to detect cancer cells and frozen cancer tissue. This AAFP consisted of two fragments: aptamer TLS11a that targets HepG2 cells, and two short extending complementary DNA sequences with a 5′- and 3′-terminus that make the aptamer in hairpin structure a capable quencher to fluorophore. The ability of the AAFP to bind specifically to cancer cells was assessed using flow cytometry, fluorescence spectroscopy and fluorescence microscopy. Its ability to bind to frozen cancer tissue was assessed using fluorescence microscopy. As a result, in the absence of cancer cells, AAFP showed minimal fluorescence, reflecting auto-quenching. In the presence of cancer cells, however, AAFP showed a strong fluorescent signal. Therefore, this AAFP may be a promising tool for sensitive and specific detection of cancer.

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Section: Discussionmentioning

confidence: 99%

An ‘activatable’ aptamer-based fluorescence probe for the detection of HepG2 cells

et al. 2017

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“…Its optical sensing is based on the interaction of a fraction of atoms/molecules on a treated surface, which strongly affects the recombination of electrons and holes within the particles, and in turn the emission response . Following this process, many analytes have been successfully detected, such as ions (Hg 2 þ , Co 2 þ , Zn 2 þ and Zr 4 þ ) (Bian et al, 2014;Gong et al, 2014a;Ren et al, 2011;Tan et al, 2012); proteins/enzymes, clenbuterol and glucose in biological fluids (Gong et al, 2014b;Wu et al, 2013;Wu et al, 2010;Zhang et al, 2013); organophosphorus pesticides (Ban et al, 2014); phenols in different media (Liu et al, 2010;Wei et al, 2015;Zhang et al, 2015b); and cathecol, domoic acid, rutin, urea, idarubicin and DNA (Bi et al, 2014;Dan et al, 2013;Ertas et al, 2015;Miao et al, 2014;Wang et al, 2013). Recently, Bian and co-workers, employing N-acetyl-L-cysteine and L-cysteine capped ZnS:Mn QDs as probes, showed the luminescent detection of L-ascorbic acid (AA) with high selectivity and long luminescence lifetime (Bian et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

L-cysteine capped ZnS:Mn quantum dots for room-temperature detection of dopamine with high sensitivity and selectivity

Diaz-Diestra

Thapa

Beltran‐Huarac

et al. 2017

Biosensors and Bioelectronics

117

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Dopamine (DA) is one of the most important catecholamine neurotransmitters of the human central nervous system, and is involved in many behavioral responses and brain functions. Below normal DA levels in biological fluids can lead to different neurodegenerative conditions. For excess DA levels, a failure in energy metabolism is indicated. In this study, a facile room-temperature phosphorescence sensor is developed to detect DA based on l-cysteine capped Mn doped ZnS quantum dots (l-cys ZnS:Mn QDs). The QDs display a prominent orange emission band peaking at ~598nm, which is strongly quenched upon addition of DA in alkaline medium. The sensor exhibits a linear working range of ~0.15-3.00μM, and a limit of detection of ~7.80nM. These results are explained in terms of a pH-dependent electron transfer process, in which the oxidized dopamine quinone functions as an efficient electron acceptor. The QDs-based sensor shows a high selectivity to DA over common interfering biomolecules (including some amino acids, ascorbic acid, chloride and glucose). The sensor has been successfully applied for the detection of DA in urine samples, yielding recoveries as high as 93%. Our findings indicate that our developed sensor exhibits high sensitivity and reproducibility to determine DA even in biological fluids where DA is at low levels, e.g., in the central nervous system, which is the usual clinical profile of a neurodegenerative disorder associated to the Parkinson's disease.

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“…Similarly, another aptamer-based turn-on thrombin biosensor was based on Mn-doped ZnS quantum dots, whose phosphorescence was restored after thrombin-aptamer recognition [176]. The system involved an efficient phosphorescence energy transfer (PET) donoracceptor pair: Mn-doped ZnS quantum dots labeled with thrombinbinding aptamers (TBA QDs) as donors, and carbon nanodots (CNDs) as acceptors.…”

Section: Thrombin Detection By Tba and Its Variants: A Biologically Rmentioning

confidence: 99%

G-quadruplex-based aptamers against protein targets in therapy and diagnostics

Platella

Riccardi

Montesarchio

et al. 2017

Biochimica et Biophysica Acta (BBA) - General Subjects

135

116

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Nucleic acid aptamers are single-stranded DNA or RNA molecules identified to recognize with high affinity specific targets including proteins, small molecules, ions, whole cells and even entire organisms, such as viruses or bacteria. They can be identified from combinatorial libraries of DNA or RNA oligonucleotides by SELEX technology, an in vitro iterative selection procedure consisting of binding (capture), partitioning and amplification steps. Remarkably, many of the aptamers selected against biologically relevant protein targets are G-rich sequences that can fold into stable G-quadruplex (G4) structures. Aiming at disseminating novel inspiring ideas within the scientific community in the field of G4-structures, the emphasis of this review is placed on: 1) recent advancements in SELEX technology for the efficient and rapid identification of new candidate aptamers (introduction of microfluidic systems and next generation sequencing); 2) recurrence of G4 structures in aptamers selected by SELEX against biologically relevant protein targets; 3) discovery of several G4-forming motifs in important regulatory regions of the human or viral genome bound by endogenous proteins, which per se can result into potential aptamers; 4) an updated overview of G4-based aptamers with therapeutic potential and 5) a discussion on the most attractive G4-based aptamers for diagnostic applications. This article is part of a Special Issue entitled "G-quadruplex" Guest Editor: Dr. Concetta Giancola and Dr. Daniela Montesarchio.

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Aptamer‐Based Turn‐On Detection of Thrombin in Biological Fluids Based on Efficient Phosphorescence Energy Transfer from Mn‐Doped ZnS Quantum Dots to Carbon Nanodots

Cited by 61 publications

References 105 publications

An ‘activatable’ aptamer-based fluorescence probe for the detection of HepG2 cells

An ‘activatable’ aptamer-based fluorescence probe for the detection of HepG2 cells

L-cysteine capped ZnS:Mn quantum dots for room-temperature detection of dopamine with high sensitivity and selectivity

G-quadruplex-based aptamers against protein targets in therapy and diagnostics

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