Aptamer based turn-off fluorescent ATP assay using DNA concatamers

Qiu, Huazhang; Liu, Zongen; Huang, Zhengjun; Chen, Min; Cai, Xueting; Weng, Shaohuang; Lin, Xinhua

doi:10.1007/s00604-015-1578-5

Cited by 11 publications

(2 citation statements)

References 27 publications

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“…5 Because of the above-mentioned aspects, there is an urgent need for the quantitative detection of ATP in biological samples. In current years, a variety of fascinating approaches have been reported for sensing ATP including electrochemiluminescence, 7 fluorescent spectroscopy, 8 SERS, 9 optomagnetic aptasensor, 10 calorimetric analysis, 11 liquid chromatography, 12 and mass spectroscopy. 13 Even though the above-said methods can attain a high detection limit and lower sensitive for the detection of ATP, these methods have certain disadvantages due to their high cost, and cumbersome operational procedure which confines their wide scope of applications.…”

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confidence: 99%

Selective and Sensitive Electrochemical Detection of ATP in Human Serum Samples at ZrO₂ Hallow Spheres Modified GCE

Kalaiyarasi

Pandian

2021

J. Electrochem. Soc.

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Zirconium oxide hollow sphere with nanoscale 3D dimensions was successfully synthesized using the carbon sphere as a hard template in which ZrOCl2 was used as a metal oxide precursor. Slow hydrolysis of zirconium oxide precursor yields a thin layer coating over the carbon sphere after subsequent calcinations at elevated temperature results hollow spheres of uniform-sized ZrO2 was obtained. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) studies were used to establish the interfacial electron transfer of the modified glassy carbon electrode (GCE). An enhanced electrochemical oxidation peak potential of ATP was noted at + 1.35 V vs Ag/AgCl at Chit-ZrO2 modified GCE which was used as an indicator electrode. A linear graph was obtained by plotting Ip vs conc. ATP at Chit-ZrO2/GCE yields good linearity in the different concentration ranges from 0.6 μM to 98.0 μM based on amperometry studies with a LOD of 70 nM and the resulting electrochemical current sensitivity of 113.11 μA·μM−1 · cm−2. Henceforth, the preparation method can be utilized for electrochemical detection of ATP which is considered as one of the biomarkers for bacterial infection in serum samples.

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confidence: 99%

Selective and Sensitive Electrochemical Detection of ATP in Human Serum Samples at ZrO₂ Hallow Spheres Modified GCE

Kalaiyarasi

Pandian

2021

J. Electrochem. Soc.

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“…A fluorescence strategy based on the discrimination in fluorescence intensity between dsDNA intercalating dyes in complex with ssDNA and dsDNA has been intensively studied in recent years. [88,101,102,187,202] The fundamental mechanism of this strategy is illustrated in Scheme 4.2. ABA is hybridized with the complementary ssDNA (here, cABA) prior to addition of a dsDNA intercalating dye (here, SG).…”

Section: Fluorescence Intensity-based Detection For Atp Using Sgmentioning

confidence: 99%

Aptasensors: From fundamentals to new sensor concepts

Liu¹

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<p>Aptamers are synthetic single-stranded (ss) oligonucleotides that bind to a broad range of targets, including cells, proteins, small molecules, toxins, metal ions, etc. with high affinity and selectivity. Aptasensors utilizing ssDNA and RNA aptamers as recognition elements have attracted significant attention in biosensing as aptamers possess excellent chemical stability, commercial availability with high purity and ease of modification with functional groups. Labeling aptamers with signal transduction groups such as fluorophores and redox markers have been extensively studied in biosensing research. However, covalent labeling processes always require complicated organic reaction, tedious preparation and separation, and expensive reagents. Thus, over the last decade, label-free aptasensors characterized by methods such as electrochemical impedance spectroscopy (EIS), surface plasmon resonance (SPR), colorimetry and fluorescence and so on have been widely developed and investigated. These efforts providing for the design of novel aptasensors, however, the lack of knowledge in understanding widely studied platforms still limits their applicability. Thus, more work is required to study the fundamentals of crucial aptasensoring platforms enabling better design, build-up and data analysis. </p>

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Aptasensor for ATP based on analyte-induced dissociation of ferrocene-aptamer conjugates from manganese dioxide nanosheets on a screen-printed carbon electrode

Tang

Hou

2016

Microchim Acta

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Aptamer based turn-off fluorescent ATP assay using DNA concatamers

Cited by 11 publications

References 27 publications

Selective and Sensitive Electrochemical Detection of ATP in Human Serum Samples at ZrO₂ Hallow Spheres Modified GCE

Selective and Sensitive Electrochemical Detection of ATP in Human Serum Samples at ZrO₂ Hallow Spheres Modified GCE

Aptasensors: From fundamentals to new sensor concepts

Aptasensor for ATP based on analyte-induced dissociation of ferrocene-aptamer conjugates from manganese dioxide nanosheets on a screen-printed carbon electrode

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Aptamer based turn-off fluorescent ATP assay using DNA concatamers

Cited by 11 publications

References 27 publications

Selective and Sensitive Electrochemical Detection of ATP in Human Serum Samples at ZrO2 Hallow Spheres Modified GCE

Selective and Sensitive Electrochemical Detection of ATP in Human Serum Samples at ZrO2 Hallow Spheres Modified GCE

Aptasensors: From fundamentals to new sensor concepts

Aptasensor for ATP based on analyte-induced dissociation of ferrocene-aptamer conjugates from manganese dioxide nanosheets on a screen-printed carbon electrode

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Selective and Sensitive Electrochemical Detection of ATP in Human Serum Samples at ZrO₂ Hallow Spheres Modified GCE

Selective and Sensitive Electrochemical Detection of ATP in Human Serum Samples at ZrO₂ Hallow Spheres Modified GCE