Aptamer-based spectrophotometric detection of kanamycin in milk

Abstract

Aminoglycoside antibiotics are widely used drugs. The residual antibiotics in animal-derived food are proven to be harmful to human health and therefore should be strictly controlled in the food industry. A spectrophotometric method based on a kanamycin-specific aptamer and gold nanoparticles (AuNPs) was developed for the quantitative detection of kanamycin. Two types of functionalized AuNPs were synthesized by self-assembly of thiol-modified single-stranded DNAs (ssDNAs), which were complementary to the 5 0 terminal and 3 0 terminal sequences of the kanamycin aptamer. As the kanamycin aptamer is mixed with these AuNPs, the AuNPs aggregate due to the hybridization of the aptamer with the complementary ssDNAs on the surface of AuNPs. However, when different concentrations of kanamycin are present, it can bind with the aptamer specifically and competitively, which leads to the disaggregation of the AuNPs aggregate, and a change in the UV-visible absorption spectrum of AuNPs. The absorbance at 527 nm is utilized to detect the concentration of kanamycin. The detection range of this method is 1-500 nM, with a detection limit of 1 nM. The developed method was then successfully employed to detect kanamycin in milk samples, and a similar response range and detection limit were obtained.

Preparation of Artificial Antigen and Development of IgY-Based Indirect Competitive ELISA for the Detection of Kanamycin Residues

“…A spectrophotometric method based on a Kana specific aptamer and gold nanoparticles (AuNPs) was developed for the quantitative detection of kanamycin. The detection range of this RSDr relative standard deviation under repeatability conditions, RSDR intralaboratory reproducibility conditions, ND not detection method is 1-500 nM, with a detection limit of 1 nM (Zhou et al 2014). Although aptamers offer many advantages over antibodies such as low batch-batch variability, small size, and easy chemical modification (Keefe et al 2010), antibody possesses high recognition ability to specific targets to small molecules.…”

Anadolu Manda Sütlerinde Kanamisin Kalıntısının LC-MS/MS İle Belirlenmesi

“…Milk is one of the most consumed foods of animal origin worldwide. However, misuse of antibiotics during treatment of milk-producing animals may cause antibiotic residues in their milk [7] . Aminoglycosides are potent antibiotics used in veterinary medicine [24] .…”

“…This antibiotic exhibits its antimicrobial effect by interfering with ribosomal RNA of gram-negative bacteria [6] . Nevertheless, kanamycin may also induce several side effects such as allergic reactions, ototoxicity, nephrotoxicity, hematopoietic system toxicity, and neuromuscular blocking in human and animals [7] . Improper use of antibiotics can cause residue risk in foods of animal origin, which may cause food safety concerns [8,9] .…”

Ultra-sensitive detection of kanamycin for food safety using a reduced graphene oxide-based fluorescent aptasensor

“…Pretreatment of milk was prepared according to the previous study38. Artificially contaminated serum and milk were prepared by spiking a standard kanamycin solution into the samples and then measuring the fluorescence signal.…”