Aptamer-based microchip electrophoresis assays for amplification detection of carcinoembryonic antigen

Li, Pan; Zhao, Jingjin; Huang, Yong; Zhao, Shulin; Liu, Yiming

doi:10.1016/j.cca.2015.09.002

Cited by 21 publications

(14 citation statements)

References 37 publications

(33 reference statements)

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“…Microfluidic capillary electrophoresis (MCE), a miniaturized platform of capillary electrophoresis (CE), can provide a simple and convenient solution for biochemical analysis compared to other methods such as high-performance liquid chromatography (HPLC), gas chromatography and CE [84]. Several research groups have exploited this MCE platform in conjunction with fluorescence detection system to develop aptamer based microfluidic biosensor [85][86][87]. For example, Pan et al [85] used this platform for rapid detection of tumor marker carcinoembryonic antigen (CEA).…”

Section: Fluorescence Detectionmentioning

confidence: 99%

“…Several research groups have exploited this MCE platform in conjunction with fluorescence detection system to develop aptamer based microfluidic biosensor [85][86][87]. For example, Pan et al [85] used this platform for rapid detection of tumor marker carcinoembryonic antigen (CEA). A laser-induced fluorescence (LIF) detection system was used to detect CEA in serum samples from colorectal cancer (CRC) patients and healthy subjects.…”

Section: Fluorescence Detectionmentioning

confidence: 99%

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Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Khan

Song

2020

Micromachines

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Aptamers are oligonucleotides or peptides that are selected from a pool of random sequences that exhibit high affinity toward a specific biomolecular species of interest. Therefore, they are ideal for use as recognition elements and ligands for binding to the target. In recent years, aptamers have gained a great deal of attention in the field of biosensing as the next-generation target receptors that could potentially replace the functions of antibodies. Consequently, it is increasingly becoming popular to integrate aptamers into a variety of sensing platforms to enhance specificity and selectivity in analyte detection. Simultaneously, as the fields of lab-on-a-chip (LOC) technology, point-of-care (POC) diagnostics, and personal medicine become topics of great interest, integration of such aptamer-based sensors with LOC devices are showing promising results as evidenced by the recent growth of literature in this area. The focus of this review article is to highlight the recent progress in aptamer-based biosensor development with emphasis on the integration between aptamers and the various forms of LOC devices including microfluidic chips and paper-based microfluidics. As aptamers are extremely versatile in terms of their utilization in different detection principles, a broad range of techniques are covered including electrochemical, optical, colorimetric, and gravimetric sensing as well as surface acoustics waves and transistor-based detection.

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Section: Fluorescence Detectionmentioning

confidence: 99%

Section: Fluorescence Detectionmentioning

confidence: 99%

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Khan

Song

2020

Micromachines

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“…These problems were overcome by the development of aptamer‐based microchip CE (aptamer MCE). Like in the case of microchip CEIA, this lab‐on‐a‐chip concept provides a short analysis time, minimized consumption, automation, high efficiency, and high throughput compared to aptamer‐based ACE . To improve the limit of detection, the combination of MCE and an aptamer immobilized onto nanoparticles can be used .…”

Section: Aptamer‐based Affinity Cementioning

confidence: 99%

“…Figure represents an example of the use of aptamer MCE for the detection of antibiotics in a food . A summary of the applications of aptamer MCE is listed in Table .…”

Section: Aptamer‐based Affinity Cementioning

confidence: 99%

Capillary electrophoresis–based immunoassay and aptamer assay: A review

2020

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Capillary electrophoresis-based immunoassay and aptamer assay: A reviewOver the last two decades, the group of techniques called affinity probe CE has been widely used for the detection and the determination of several types of biomolecules with high sensitivity. These techniques combine the low sample consumption and high separation power of CE with the selectivity of the probe to the target molecule. The assays can be defined according to the type of probe used: CE immunoassays, with an antibody as the probe, or aptamer-based CE, with an aptamer as the probe. Immunoassays are generally divided into homogeneous and heterogeneous groups, and homogeneous variant can be further performed in competitive or noncompetitive formats. Interacting partners are free in solution at homogeneous assay, as opposed to heterogeneous analyses, where one of them is immobilized onto a solid support. Highly sensitive fluorescence, chemiluminescence or electrochemical detections were typically used in this type of study. The use of the aptamers as probes has several advantages over antibodies such as shorter generation time, higher thermal stability, lower price, and lower variability. The aptamer-based CE technique was in practice utilized for the determination of proteins in biological fluids and environmentally or clinically important small molecules. Both techniques were also transferred to microchip. This review is focused on theoretical principles of these techniques and a summary of their applications in research.Abbreviations: Ag, antigen; CEIA, CE immunoassay; MCE, microchip CE; SELEX, systematic evolution of ligands by exponential enrichment and they are produced by the immune system of living organisms. Ag that start the immunological response are also called immunogens. These Ab-Ag complexes are stabilized by dipole-dipole, van der Waals or hydrophobic interactions and hydrogen bonds [5,6].The most often used immunoassays are ELISA with Ab or Ag immobilized onto solid support (plates, glass fibers, or plastic tubes), immunofluorescence assay, fluorescence polarization immunoassay, luminescence immunoassay, microparticle enzyme immunoassay, and biosensors. The major disadvantages of immunoassays are the tedious process, time-consuming reactions, and problems with reproducibility and nonspecific interactions [6].CE is a powerful analytical method for the separation of a wide range of molecules. The coupling of CE and immunoassay in the new CE immunoassay (CEIA) technique provided both separation power and specificity of interactions. Other advantages are the possibility of multianalyte analyses, automation, low sample consumption, high speed, flexibility, a lower amount of false-positive results, and also the possibility of connection with highly sensitive detectors [5].In CEIA, the Fab fragments of Ab containing Ag-specific regions are more often used than the whole monoclonal and Color online: See article online to view Figs. 4 and 7 in color.

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“…Carcinoembryonic antigen (CEA) is a glycoprotein of tumor tissues, mainly in colon but also in pancreas, breast, stomach, and lung tumors . As CEA is extremely rarely detectable in normal serum, CEA is one of the most common tumor markers, and its detection in the serum is very helpful for early diagnosis, evaluation of curative effect, and judgment of tumor recrudescence . Immunological methods, based on antigen–antibody reaction, have been used for CEA detection in serum, such as radioimmunoassay, electrochemical immunoassay, chemiluminescent immunoassay, and enzyme immunoassay .…”

Section: Introductionmentioning

confidence: 99%

An Aptamer‐based Self‐Catalytic Colorimetric Assay for Carcinoembryonic Antigen

Zou

Wang

2017

Bulletin Korean Chem Soc

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We report a visualization assay for carcinoembryonic antigen (CEA). The assay was prepared based on target‐specific recognition, aptamer‐based DNAzyme self‐catalysis, and colorimetric reaction. Aptamer of CEA was gathered at the surface of magnetic beads for sandwiched co‐conjugate when CEA was added. The second aptamer (Apt2) on the beads was folded into G‐quadruplex by KCl to make G‐quadruplex‐hemin DNAzyme with hemin. Apt2‐based DNAzyme catalyzed H2O2‐mediated oxidation of 3,3′,5,5′‐tetramethylbenzidine, resulting in color changes and significant increase in absorption intensity at 450 nm (ΔA450nm). Yellow products can be observed with naked eyes when over 1.0 ng/mL CEA was added and there was a linear correlation between ΔA450 nm and CEA concentrations in the range of 0.1–8.0 ng/mL with the detection limit of 0.06 ng/mL. The proposed method was then used to determine CEA in human serum from healthy people and tumor patients, revealing a correlation with data from the electrochemiluminescent immunoassay. Owing to its simplicity, selectivity, and low‐cost, this method is a promising candidate for detecting CEA and clinical diagnosis of cancer.

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Aptamer-based microchip electrophoresis assays for amplification detection of carcinoembryonic antigen

Cited by 21 publications

References 37 publications

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Capillary electrophoresis–based immunoassay and aptamer assay: A review

An Aptamer‐based Self‐Catalytic Colorimetric Assay for Carcinoembryonic Antigen

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