Aptamer-based lateral flow assay on-site biosensors

Huang, Lei; Tian, Shulin; Zhao, Wenhao; Liu, Ke; Ma, Xing; Guo, Jinhong

doi:10.1016/j.bios.2021.113279

Cited by 75 publications

(50 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The screening procedures of structure similarity, docking, and MD simulations can be applied to perform the in-silico selection of aptamers for screening a large number of mutated sequences. The computational results obtained from our study reveal that the RBD-1CM1 aptamer can have strong interactions with the RBD of the S1 subunit, which also has the potential for diagnosis applications, such as in rapid test kits [ 12 ] and aptamer-based biosensors [ 11 ], or for the treatment of SARS-CoV-2 [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…Compared with antibodies, aptamers have several advantages, such as robustness, low cost, reusability, ease of chemical modification, low batch-to-batch variability, and low immunogenicity [ 9 , 10 ]. Therefore, aptamers have been utilized in the detection of SARS-CoV-2 S protein using an electrochemical biosensor [ 11 ], and the development of aptamer-based lateral flow assays (LFAs) can provide the rapid detection of SARS-CoV-2 to address the COVID-19 pandemic [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In-Silico Selection of Aptamer Targeting SARS-CoV-2 Spike Protein

Lin

Chen

Hwu

et al. 2022

IJMS

View full text Add to dashboard Cite

Aptamers are single-stranded, short DNA or RNA oligonucleotides that can specifically bind to various target molecules. To diagnose the infected cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in time, numerous conventional methods are applied for viral detection via the amplification and quantification of DNA or antibodies specific to antigens on the virus. Herein, we generated a large number of mutated aptamer sequences, derived from a known sequence of receptor-binding domain (RBD)-1C aptamer, specific to the RBD of SARS-CoV-2 spike protein (S protein). Structural similarity, molecular docking, and molecular dynamics (MD) were utilized to screen aptamers and characterize the detailed interactions between the selected aptamers and the S protein. We identified two mutated aptamers, namely, RBD-1CM1 and RBD-1CM2, which presented better docking results against the S protein compared with the RBD-1C aptamer. Through the MD simulation, we further confirmed that the RBD-1CM1 aptamer can form the most stable complex with the S protein based on the number of hydrogen bonds formed between the two biomolecules. Based on the experimental data of quartz crystal microbalance (QCM), the RBD-1CM1 aptamer could produce larger signals in mass change and exhibit an improved binding affinity to the S protein. Therefore, the RBD-1CM1 aptamer, which was selected from 1431 mutants, was the best potential candidate for the detection of SARS-CoV-2. The RBD-1CM1 aptamer can be an alternative biological element for the development of SARS-CoV-2 diagnostic testing.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In-Silico Selection of Aptamer Targeting SARS-CoV-2 Spike Protein

Lin

Chen

Hwu

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Early detection of serum biomarker proteins is of great significance for the early diagnosis and treatment of diseases, such as cancer and viral infection [1] , [2] , [3] . Protein can be measured by different methods including enzyme-linked immunosorbent assays (ELISA) [4] , mass spectrometry-based proteomics [5] , [6] , surface plasmon resonance (SPR) [7] , electrochemiluminescence [8] and the lateral flow assay-based method [9] , [10] . Among them, ELISA has been the classical method for the quantitative assessment of water-soluble proteins.…”

Section: Introductionmentioning

confidence: 99%

“…However, the time-consuming washing steps in such assays not only make them labor-intensive but also prevent analysis in real-time, limiting further application. Although the lateral flow assay-based method is simple and suitable for rapid detection [15] , [16] , it can only be used for semi-quantitative analysis and isn't sensitive enough, which is not suitable for low-concentration protein quantification or early diagnosis [9] , [10] .…”

Section: Introductionmentioning

confidence: 99%

Allosteric aptasensor-initiated target cycling and transcription amplification of light-up RNA aptamer for sensitive detection of protein

Song¹,

Yuan²,

Tan³

et al. 2022

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

“…DNA-modified gold nanoparticles (GNPs@DNA) obtained usually through the alkanethiol adsorption 1 have an important role in many fields, including DNA-programmed assembly, 2,3 biosensors, 4 biological imaging, 5,6 and therapeutics. 7,8 The surface density of DNA molecules on the GNPs surface is highly related to the sensing sensitivity of GNPs@DNA-based sensors, 9 DNA hybridization efficiency, 10 cellular uptake capability, 11 etc.…”

Section: Introductionmentioning

confidence: 99%