Aptamer‐based high‐throughput screening model for medicinal plant drugs against SGIV

Liu, Mingzhu; Xiao, Haifang; Wu, Shao-Yi; Yu, Qing; Li, Pengfei

doi:10.1111/jfd.13254

Cited by 7 publications

(9 citation statements)

References 12 publications

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“…Both of these methods proved that 20 natural component isolated from different medicinal plants had antiviral activities against SGIV infection. The anti-SGIV effects of components 1-14, 16, and 20 by Q2-AHTS were consistent with those measured by RT-qPCR, which was consistent with previous studies [40]. Nevertheless, the antiviral effects of components 15 and 17-19 differed when measured by Q2-AHTS and RT-qPCR.…”

Section: Discussionsupporting

confidence: 90%

“…RT-qPCR showed that viral MCP gene expression increased and showed a significant difference with the increasing virus titer and time of SGIV infection. The results were consistent with a previous published study [40]. By comparing the specificity and sensitivity of RT-qPCR and Q2-AHTS for identifying SGIV infection, we found that two screening methods had the same trends at 24 and 48 hpi of SGIV infection and had different trends at 6 and 12 hpi, indicating that the detection accuracy of AHTS was not comparable to that of RT-qPCR.…”

Section: Discussionsupporting

confidence: 89%

“…The fluorescence results showed that the binding of aptamer Q2 to SGIVinfected cells (MOI = 0.5) was significantly increased at the 24 and 48 hpi, which was consistent with confocal observation. Aptamer Q2 is less sensitive to SGIV than aptamer LYGV1 [40], which may be because the dissociation equilibrium constant (Kd) of aptamer LYGV1 (Kd = 32.55 nmol/L) is higher than that of aptamer Q2 (Kd = 12.09 nmol/L). Second, we explored the specificity and sensitivity of detecting SGIV infection by RT-qPCR and Q2-AHTS.…”

Section: Discussionmentioning

confidence: 99%

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Aptamer-Based High-Throughput Screening Model for Efficient Selection and Evaluation of Natural Ingredients against SGIV Infection

Wei

Guo

Liu

et al. 2022

Viruses

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Singapore grouper iridovirus (SGIV) causes high economic losses in mariculture. Effective drugs for managing SGIV infection are urgently required. Medicinal plant resources are rich in China. Medicinal plants have a long history and significant curative effects in the treatment of many diseases. Reverse-transcription quantitative real-time PCR is the most commonly used method for detecting virus infection and assessing antiviral efficacy with high accuracy. However, their applications are limited due to high reagent costs and complex time-consuming operations. Aptamers have been applied in some biosensors to achieve the accurate detection of pathogens or diseases through signal amplification. This study aimed to establish an aptamer-based high-throughput screening (AHTS) model for the efficient selection and evaluation of medicinal plants components against SGIV infection. Q2-AHTS is an expeditious, rapid method for selecting medicinal plant drugs against SGIV, which was characterized as being dram, high-speed, sensitive, and accurate. AHTS strategy reduced work intensity and experimental costs and shortened the whole screening cycle for effective ingredients. AHTS should be suitable for the rapid selection of effective components against other viruses, thus further promoting the development of high-throughput screening technology.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Aptamer-Based High-Throughput Screening Model for Efficient Selection and Evaluation of Natural Ingredients against SGIV Infection

Wei

Guo

Liu

et al. 2022

Viruses

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“…In addition, aptamers have been used to establish aptamer‐based screening models. However, there is only one study reported that Liu, Xiao, et al (2020) constructed a high‐throughput drug screening technology against SGIV using aptamer LYGV1. The high‐throughput model screened 22 medicinal plant drugs at one time and most of them have anti‐SGIV effects.…”

Section: Discussionmentioning

confidence: 99%

Study on aptamer based high throughput approach identifies natural ingredients against RGNNV

Wei

Liu

et al. 2022

Journal of Fish Diseases

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Nervous necrosis virus (NNV) is one of the most destructive pathogens in marine fish aquaculture and is capable of infecting more than 50 fish species worldwide, which resulted in great economic losses. Effective drugs for managing NNV infection are urgently required. Medicinal plants have been known for thousands of years and benefit of medicinal plants against pathogens in aquaculture have emerged. Nowadays, the most commonly used method for detecting virus infection and assessing antiviral drugs efficacy is reverse transcription‐quantitative real‐time PCR. However, the application is limited on account of high reagent costs, complex time‐consuming operations and long detection time. Aptamers have been widely applied in application of pathogens or diseases diagnosis and treatments because of high specificity, strong affinity, good stability, easy synthesized and low costs. This study aimed to establish an aptamer (GBN34)‐based high‐throughput screening (GBN34‐AHTS) model for efficient selection and evaluation of natural ingredients against NNV infection. GBN34‐AHTS is an expeditious rapid method for selecting natural ingredients against NNV, which is characterized with high‐speed, dram, sensitive and accurate. AHTS strategy could reduce work intensity and experimental costs and shorten the whole screening cycle of effective ingredients. AHTS should be suitable for rapid selection of effective ingredients against other viruses, which is important for improving the prevention and controlling of aquatic diseases.

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“…It is highly contagious; grouper fry are susceptible to this disease, with a large number of deaths in a short period of time after the disease [115]. SGIV is highly pathogenic, and high morbidity and mortality rates in aquatic animals have been caused by iridovirus infections worldwide in recent years [116]. The disease caused by SGIV results in significant economic losses to the aquaculture industry and poses a major threat to global biodiversity.…”

Section: Medicinal Plants Against Dna Viruses 221 Medicinal Plants Ag...mentioning

confidence: 99%

Review of Medicinal Plants and Active Pharmaceutical Ingredients against Aquatic Pathogenic Viruses

Liao

Huang

Han

et al. 2022

Viruses

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Aquaculture offers a promising source of economic and healthy protein for human consumption, which can improve wellbeing. Viral diseases are the most serious type of diseases affecting aquatic animals and a major obstacle to the development of the aquaculture industry. In the background of antibiotic-free farming, the development and application of antibiotic alternatives has become one of the most important issues in aquaculture. In recent years, many medicinal plants and their active pharmaceutical ingredients have been found to be effective in the treatment and prevention of viral diseases in aquatic animals. Compared with chemical drugs and antibiotics, medicinal plants have fewer side-effects, produce little drug resistance, and exhibit low toxicity to the water environment. Most medicinal plants can effectively improve the growth performance of aquatic animals; thus, they are becoming increasingly valued and widely used in aquaculture. The present review summarizes the promising antiviral activities of medicinal plants and their active pharmaceutical ingredients against aquatic viruses. Furthermore, it also explains their possible mechanisms of action and possible implications in the prevention or treatment of viral diseases in aquaculture. This article could lay the foundation for the future development of harmless drugs for the prevention and control of viral disease outbreaks in aquaculture.

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Aptamer‐based high‐throughput screening model for medicinal plant drugs against SGIV

Cited by 7 publications

References 12 publications

Aptamer-Based High-Throughput Screening Model for Efficient Selection and Evaluation of Natural Ingredients against SGIV Infection

Aptamer-Based High-Throughput Screening Model for Efficient Selection and Evaluation of Natural Ingredients against SGIV Infection

Study on aptamer based high throughput approach identifies natural ingredients against RGNNV

Review of Medicinal Plants and Active Pharmaceutical Ingredients against Aquatic Pathogenic Viruses

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