Aptamer-Based Detection Methodology Studies in Food Safety

Song, Shang-Hong; Gao, Zhifei; Guo, Xin; Chen, Guanhua

doi:10.1007/s12161-019-01437-3

Cited by 45 publications

(19 citation statements)

References 175 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A multitude of reviews have been published lately describing the progress in aptasensors intended for food quality and safety monitoring [ 2 , 16 , 42 , 190 , 243 , 245 , 246 , 247 , 248 , 249 , 250 ]. This paper brings a new perspective on this subject by emphasizing the contribution of aryldiazonium chemistry in developing aptasensing assays for commonly encountered food contaminants.…”

Section: Discussionmentioning

confidence: 99%

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

View full text Add to dashboard Cite

Food safety monitoring assays based on synthetic recognition structures such as aptamers are receiving considerable attention due to their remarkable advantages in terms of their ability to bind to a wide range of target analytes, strong binding affinity, facile manufacturing, and cost-effectiveness. Although aptasensors for food monitoring are still in the development stage, the use of an electrochemical detection route, combined with the wide range of materials available as transducers and the proper immobilization strategy of the aptamer at the transducer surface, can lead to powerful analytical tools. In such a context, employing aryldiazonium salts for the surface derivatization of transducer electrodes serves as a simple, versatile and robust strategy to fine-tune the interface properties and to facilitate the convenient anchoring and stability of the aptamer. By summarizing the most important results disclosed in the last years, this article provides a comprehensive review that emphasizes the contribution of aryldiazonium chemistry in developing electrochemical aptasensors for food safety monitoring.

show abstract

Section: Discussionmentioning

confidence: 99%

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

View full text Add to dashboard Cite

show abstract

“…Concerns about the pesticide residues in agricultural products have been raised in recent years because of increasing health concerns and public interest in food quality and safety [20]. Thus, rapid, convenient, and accurate analytical methods for the detection and quantification of pesticides are urgently required.…”

Section: E Ppix and Plantsmentioning

confidence: 99%

“…Thus, rapid, convenient, and accurate analytical methods for the detection and quantification of pesticides are urgently required. Recently, the aggregation effect of pesticides on the porphyrins gold nanoparticles was successfully applied for the rapid and accurate determination of multicomponent pesticide residues (Paraquat, Dipterex, Dursban, methyl thiophanate, and Cartap) in food samples by observing changes in the porphyrin (422 nm) UV-vis spectra [20].…”

Section: E Ppix and Plantsmentioning

confidence: 99%

Protoporphyrin IX: An Endogenous Theranostic Compound

Courrol

2021

2021 SBFoton International Optics and Photonics Conference (SBFoton IOPC)

View full text Add to dashboard Cite

Porphyria, cancer, and atherosclerosis patients manifest increased concentration of protoporphyrin IX (PpIX) in tissues and blood, and PpIX fluorescence can be used to diagnose these diseases. This review will describe the role of PpIX inside the cells and organisms. Diagnosis and therapy approach using PpIX will be described. Finally, we will also evaluate if PpIX could be used to diagnose and treat Covid-19 since an abnormal phenomenon related to hemoglobin dysfunction was observed in the patients with this disease.

show abstract

“…Aptamers have emerged as a class of recognition elements that can bind with a wide range of analytes, such as proteins, metal ions, organic molecules and cells (Grechkin et al 2020;Jia et al 2020;Saad et al 2020;Stojanovic and Landry 2002;Sun et al 2019a;Wang et al 2020;Yao et al 2020;Zhang et al 2020b). Aptamers have been extensively studied as biomaterials in numerous diagnostic applications (e.g., detection of infectious agents, food inspection, antigens/toxins (bacteria), biomarkers (cancer)) (Chandola et al 2016;Ratajczak et al 2018Ratajczak et al , 2019Song et al 2019;Zheng et al 2019). Aptamers in comparison with their antibody counterparts exhibit several advantages for the fabrication of biosensors particularly the colorimetric-based sensor arrays.…”

Section: Introductionmentioning

confidence: 99%

Visual detection of tropomyosin, a major shrimp allergenic protein using gold nanoparticles (AuNPs)-assisted colorimetric aptasensor

Pavase

Lin

Soomro

et al. 2021

Mar Life Sci Technol

View full text Add to dashboard Cite

A gold nanoparticle-based label-free colorimetric assay was developed to detect the shrimp allergenic protein tropomyosin (TM), an important biomarker responsible for severe clinical reactivity to shellfish. In a gold nanoparticles (AuNPs)-tropomyosin-binding aptamer (TMBA) complex, the aptamer adsorbs onto the surface of AuNPs and dissociates in the presence of TM. In addition, AuNPs tend to aggregate in the presence of ionic salt, revealing a color change (i.e., wine-red to purple/ blue) with a shift in the maximum absorption peak from 520 nm. In the presence of specific binding TM, the aptamer folds into a tertiary structure where it more efficiently stabilizes AuNPs toward the salt-induced aggregation with a hypsochromic shift in the absorption spectra compared to the stabilized AuNPs by aptamer alone. Based on the aggregation and sensitive spectral transformation principle, the AuNPs-based colorimetric aptasensor was successfully applied to detect TM with a range of 10-200 nmol/L and a low detection limit of 40 nmol/L in water samples. The reliability, selectivity, and sensitivity of the aptasensor was then tested with food samples spiked with TM. The observed detection limit was as low as 70 nmol/L in shrimp, 90 nmol/L in tofu, and 80 nmol/L in eggs, respectively. We anticipate the proposed AuNPs-based colorimetric aptasensor assay possesses a high potential for the easy and efficient visual colorimetric detection of TM.

show abstract

Aptamer-Based Detection Methodology Studies in Food Safety

Cited by 45 publications

References 175 publications

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Protoporphyrin IX: An Endogenous Theranostic Compound

Visual detection of tropomyosin, a major shrimp allergenic protein using gold nanoparticles (AuNPs)-assisted colorimetric aptasensor

Contact Info

Product

Resources

About