Aptamer-based colorimetric biosensing of dopamine using unmodified gold nanoparticles

Zheng, Yu; Wang, Yong; Yang, Xiurong

doi:10.1016/j.snb.2011.03.077

Cited by 221 publications

(125 citation statements)

References 32 publications

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“…This phenomenon suggested that the aptamer AFB1 could serve as a stabilizer and protected AuNPs from salt-induced aggregation. 32 The state of AuNPs was further confirmed by the TEM image ( Fig. 2A) from which we could observe that AuNPs remained dispersed in the presence of AFB1 aptamer.…”

Section: Colorimetric Methods For Detecting Aflatoxin B1mentioning

confidence: 58%

Aptamer-based Colorimetric and Chemiluminescence Detection of Aflatoxin B1 in Foods Samples

Hosseini

Khabbaz²,

Dadmehr³

et al. 2015

ACSi

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We developed a new biosensor for the detection of aflatoxin B1(AFB1) based on the interaction of gold nanoparticles (AuNPs) with the aptamer. Aggregation of AuNPs was induced by desorption of the AFB1 binding aptamer from the surface of AuNPs as a result of the aptamer target interaction leading to the color change of AuNPs from red to purple. The linear range of the colorimetric aptasensor covered a large variation of AFB1 concentrations from 80 to 270 nM and the detection limit of 7 nM was obtained. Also, the catalytic activity of the aggregated AuNPs greatly enhanced the chemiluminescence (CL) reaction, where the detection limit was determined at 0.5 nM with a regression coefficient of R 2 = 0.9921. We have also shown that the sensitivity of detection was increased by employing CL and using the catalytic activity of aggregated AuNPs, during luminol-hydrogen peroxide reaction. Therefore the proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, introducing a viable alternative for rapid screening of toxin in foods.

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Section: Colorimetric Methods For Detecting Aflatoxin B1mentioning

confidence: 58%

Aptamer-based Colorimetric and Chemiluminescence Detection of Aflatoxin B1 in Foods Samples

Hosseini

Khabbaz²,

Dadmehr³

et al. 2015

ACSi

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show abstract

“…[8][9][10][11][12][13][14][15][16][17][18] Among these sensing technologies, electrochemical aptasensors have attracted particular attention because they provide a simple, sensitive, and selective platform for thrombin detection. A SPR spectroscopic aptasensor allows use of a microsecond time-resolved change of SPR reflectance.…”

Section: -7mentioning

confidence: 99%

Spectroscopic and Electrochemical Detection of Thrombin/5'-SH or 3'-SH Aptamer Immobilized on (porous) Gold Substrates

Park

Seung²,

Kim³

et al. 2012

Bulletin of the Korean Chemical Society

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Thrombin is a serine protease that catalyzes the conversion of soluble fibrinogen to insoluble fibrin, and thus induces physiological and pathological blood coagulation. Therefore, it is important to detect thrombin in blood serum for purposes of diagnosis. To achieve this goal, it has been suggested that a 15-mer aptamer strongly binds with thrombin to form a G-quartet structure of the aptamer. Generally, 5'-end thiol-functionalized aptamer has been used as an anti-thrombin binder. Herein, we evaluate the possibility of utilizing a 3'-SH aptasensor for thrombin detection using SPR spectroscopy, and compare the enhancement of the electrochemical signal of the thrombin-aptamer bound on a porous gold substrate. Although the two aptamers have similar configurations, in SPR analysis, the 3'-SH aptamer was a effective aptasensor as well as 5'-SH aptamer.Results from electrochemical analysis showed that the porous gold substrate acted as a good substrate for an aptasensor and demonstrated 5-fold enhancement of current change, as compared to gold thin film.

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“…Aptamers are single-stranded oligonucleotides of DNA or RNA sequences obtained by an in vitro selection process named the systematic evolution of ligands by exponential enrichment (SELEX), which would bind to the surface of gold nanoparticles (AuNPs) through electrostatic interaction and specially forming the individual 3D structures. 8,9 The selected aptamers were easily obtained by chemical synthesis, which provided several advantages such as low cost, inherent selectivity, high stability, and a greater surface density of receptors on AuNPs. 10 Compared with aptamers, antibodies are easily affected by temperature and pH variations and undergo irreversible denaturation, 11 while aptamers are stable against various variants.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-based Colorimetrie Biosensing of Ochratoxin A in Fortified White Grape Wine Sample Using Unmodified Gold Nanopartieles

et al. 2017

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Aptamer-based colorimetric biosensing of dopamine using unmodified gold nanoparticles

Cited by 221 publications

References 32 publications

Aptamer-based Colorimetric and Chemiluminescence Detection of Aflatoxin B1 in Foods Samples

Aptamer-based Colorimetric and Chemiluminescence Detection of Aflatoxin B1 in Foods Samples

Spectroscopic and Electrochemical Detection of Thrombin/5'-SH or 3'-SH Aptamer Immobilized on (porous) Gold Substrates

Aptamer-based Colorimetrie Biosensing of Ochratoxin A in Fortified White Grape Wine Sample Using Unmodified Gold Nanopartieles

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