Aptamer-Based Carboxyl-Terminated Nanocrystalline Diamond Sensing Arrays for Adenosine Triphosphate Detection

Suaebah, Evi; Naramura, Takuro; Myodo, Miho; Hasegawa, Masataka; Shoji, Shuichi; Buendia, Jorge J.; Kawarada, Hiroshi

doi:10.3390/s17071686

Cited by 5 publications

(5 citation statements)

References 35 publications

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“…The systematic evolution of ligands by exponential enrichment (SELEX) is used to isolate aptamers with a high affinity for a molecular target from approximately 10 12 –10 15 oligonucleotide combinations [ 45 , 46 , 47 ]. In general, the SELEX process consists of three steps: creating a “library”, binding and separation, and amplification.…”

Section: Resultsmentioning

confidence: 99%

Detection of Biomarker Using Aptasensors to Determine the Type of Diabetes

Mulyani,

Maksum

2023

Diagnostics

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Diabetes mellitus (DM) is a metabolic disorder characterized by elevated blood glucose levels. This disease is so serious that many experts refer to it as the “silent killer”. The early detection of diabetes mellitus, whether type 1, type 2 or mitochondrial, is crucial because it can improve the success of treatment and the quality of life for patients. Aptamer-based biosensor diagnosis methods have been widely developed because they have high sensitivity and selectivity in detecting biomarkers of various diseases. Aptamers are short sequences of oligonucleotides or proteins that recognize specific ligands and bind to various target molecules, ranging from small ions to large proteins. They are promising diagnostic molecules due to their high sensitivity and selectivity, ease of modification, low toxicity, and high stability. This article aims to summarize the progress of detection methods, including detection principles, sensitivity, selectivity, and the performance of detection devices, to distinguish between types of diabetes mellitus using electrochemical aptasensors with biomarkers such as glucose, insulin, HbA1c, GHSA, and ATP.

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Section: Resultsmentioning

confidence: 99%

Detection of Biomarker Using Aptasensors to Determine the Type of Diabetes

Mulyani,

Maksum

2023

Diagnostics

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“…Only covalently bonded molecules with high stability remained on the diamond surface. (9,10) The intensity of the circles on the diamond surface increased with the length of hybridization. First, the fluorescence signal occurred during hybridization.…”

Section: Aptasensor For E2 Detectionmentioning

confidence: 99%

“…(7,8) Research into the application of aptamers to the sensing of small molecules has resulted in the development of advanced biosensors. (9)(10)(11)(12)(13)(14)(15) Aptamers are single-stranded DNA or RNA molecules with various shapes owing to their tendency observed in the systematic evolution of ligands by the exponential enrichment (SELEX) process. (16) Hence, they are extremely good for binding with a target with high affinity, selectivity, and specificity.…”

Section: Introductionmentioning

confidence: 99%

“…(12,32,33) Fluorescence sensing is the simplest and fastest method for the detection of small molecules using diamond as a sensing platform. (9,10) As an alternative, surface functionalization with an aptasensor has been explored for the detection of various small molecules and proteins. (34,35) Several biosensors for the detection of E2 have been deployed using E2 aptamers.…”

Section: Introductionmentioning

confidence: 99%

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Functionalization of a Diamond Surface through N2 and H2 Irradiation for Estrogen (17��-estradiol) Aptamer Sensing

Suaebah¹,

Hasegawa

Buendia³

et al. 2019

Sensors and Materials

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A label-free fluorescence aptasensor for the determination of 17β-estradiol (E2) was developed. The aptasensor was prepared by the functionalization of a diamond surface using a nitrogen radical beam (NRB) system to produce amine terminals on the diamond itself. The fabrication of the sensor was performed by photolithography to produce a dot pattern, which was used as a sensing area for activated biomolecules. The supporting DNA strands were immobilized and an aptamer was hybridized to prepare a detection pair to bind with any E2 molecule, as the aptamer captures the E2 molecule naturally. The fluorescence microscopy signal was used for the detection of E2. The biomolecule activities were determined through special treatment, with three different stages evident in the fluorescence result, namely, hybridization, detection, and denaturation. When E2 was detected by the aptamer, the intensity of the fluorescence signal decreased because of the aptamer binding with the E2 molecule. E2 was detected by determining the fluorescence signal intensity of the dot pattern. When the aptamer was released from the supporting DNA and formed a complex with the E2 molecule, the intensity of the dot pattern decreased rapidly. The difference between the two signals indicated the number of E2 molecules bound to the aptamer. The proposed method is simple and powerful for the detection of small molecules with high sensitivity by utilizing the basic reaction of aptamers.

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“…Aptamers are oligonucleotide sequences and can be screened by the technology of a systematic evolution of ligands with exponential enrichment (SELEX) in vitro [ 9 ]. The aptamers and the specific target substances can interact with a high affinity and specificity [ 10 , 11 ]. Currently, different aptamers specific for metal ions [ 12 ], small molecules [ 13 ], drugs [ 14 ], proteins [ 15 ] and cells [ 16 ] have been reported.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Aptasensor for Highly Specific Detection of ATP Using a Newly Screened Aptamer

Chen

Feng

Chen

et al. 2022

Sensors

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Owing to the significant roles of adenosine triphosphate (ATP) in diverse biological processes, ATP level is used to research and evaluate the physiological processes of organisms. Aptamer-based biosensors have been widely reported to achieve this purpose, which are superior in their flexible biosensing mechanism, with a high sensitivity and good biocompatibility; however, the aptamers currently used for ATP detection have a poor ability to discriminate ATP from adenosine diphosphate (ADP) and adenosine monophosphate (AMP). Herein, an ATP-specific aptamer was screened and applied to construct a fluorescent aptasensor for ATP by using graphene oxide (GO) and strand displacement amplification (SDA). The fluorescence intensity of the sensor is linearly related to the concentration of ATP within 0.1 μM to 25 μM under optimal experimental conditions, and the detection limit is 33.85 nM. The biosensor exhibits a satisfactory specificity for ATP. Moreover, the experimental results indicate that the biosensor can be applied to determine the ATP in human serum. In conclusion, the screened aptamer and the biosensor have promising applications in the determination of the real energy charge level and ATP content in a complex biological system.

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Aptamer-Based Carboxyl-Terminated Nanocrystalline Diamond Sensing Arrays for Adenosine Triphosphate Detection

Cited by 5 publications

References 35 publications

Detection of Biomarker Using Aptasensors to Determine the Type of Diabetes

Detection of Biomarker Using Aptasensors to Determine the Type of Diabetes

Functionalization of a Diamond Surface through N2 and H2 Irradiation for Estrogen (17��-estradiol) Aptamer Sensing

Fluorescent Aptasensor for Highly Specific Detection of ATP Using a Newly Screened Aptamer

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