Aptamer-Based Biosensors to Detect Aquatic Phycotoxins and Cyanotoxins

Cunha, Isabel; Biltes, Rita; Sales, M. Goreti F.; Vasconcelos, Vı́tor

doi:10.3390/s18072367

Cited by 67 publications

(29 citation statements)

References 106 publications

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“…Among various biosensor formats, optical and electrochemical sensors are mostly developed for aquatic water analysis. Cunha et al reported an optical sensor that exploits DNA aptamer and quantum dots (QDs) for real-time onsite detection of aquatic toxins produced by marine and freshwater microorganisms (cyanobacteria, dinoflagellates, and diatoms) [61]. This sensor exploits fluorescence resonance energy transfer (FRET) between a quencher-labeled DNA aptamer and QDs, in the presence of target toxin.…”

Section: Biosensors For Cyanotoxins and Cyanobacteriamentioning

confidence: 99%

Sensors, Biosensors, and Analytical Technologies for Aquaculture Water Quality

Sutarlie

Loh

2020

Research

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In aquaculture industry, fish, shellfish, and aquatic plants are cultivated in fresh, salt, or brackish waters. The increasing demand of aquatic products has stimulated the rapid growth of aquaculture industries. How to effectively monitor and control water quality is one of the key concerns for aquaculture industry to ensure high productivity and high quality. There are four major categories of water quality concerns that affect aquaculture cultivations, namely, (1) physical parameters, e.g., pH, temperature, dissolved oxygen, and salinity, (2) organic contaminants, (3) biochemical hazards, e.g., cyanotoxins, and (4) biological contaminants, i.e., pathogens. While the physical parameters are affected by climate changes, the latter three are considered as environmental factors. In this review, we provide a comprehensive summary of sensors, biosensors, and analytical technologies available for monitoring aquaculture water quality. They include low-cost commercial sensors and sensor network setups for physical parameters. They also include chromatography, mass spectrometry, biochemistry, and molecular methods (e.g., immunoassays and polymerase chain reaction assays), culture-based method, and biophysical technologies (e.g., biosensors and nanosensors) for environmental contamination factors. According to the different levels of sophistication of various analytical techniques and the information they can provide (either fine fingerprint, highly accurate quantification, semiquantification, qualitative detection, or fast screening), we will comment on how they may be used as complementary tools, as well as their potential and gaps toward current demand of real-time, online, and/or onsite detection.

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Section: Biosensors For Cyanotoxins and Cyanobacteriamentioning

confidence: 99%

Sensors, Biosensors, and Analytical Technologies for Aquaculture Water Quality

Sutarlie

Loh

2020

Research

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“…Although aptasensors have been widely used in different fields [102][103][104][105][106][107][108], great efforts still need to be made in the following respects: (1) the optimization of aptamer selection process. Although some aptamers for marine toxins have been selected, it is still not sufficient for a large amount of marine toxins with categories of more than 1000.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Marine Toxins Detection by Biosensors Based on Aptamers

Wei

Liu

Zhang

et al. 2019

Toxins

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Marine toxins cause great harm to human health through seafood, therefore, it is urgent to exploit new marine toxins detection methods with the merits of high sensitivity and specificity, low detection limit, convenience, and high efficiency. Aptasensors have emerged to replace classical detection methods for marine toxins detection. The rapid development of molecular biological approaches, sequencing technology, material science, electronics and chemical science boost the preparation and application of aptasensors. Taken together, the aptamer-based biosensors would be the best candidate for detection of the marine toxins with the merits of high sensitivity and specificity, convenience, time-saving, relatively low cost, extremely low detection limit, and high throughput, which have reduced the detection limit of marine toxins from nM to fM. This article reviews the detection of marine toxins by aptamer-based biosensors, as well as the selection approach for the systematic evolution of ligands by exponential enrichment (SELEX), the aptamer sequences. Moreover, the newest aptasensors and the future prospective are also discussed, which would provide thereotical basis for the future development of marine toxins detection by aptasensors.Key Contribution: This article reviews systematically the toxicity of marine toxins, the development of aptarmer-based biosensors, and progress in the marine toxin detection by aptasensors.Toxins 2020, 12, 1 2 of 22 selection technology and biosensor fabrication technology offer a new solution for the detection of marine toxins with high efficiency and sensitivity [13][14][15][16][17].Marine toxins are a class of small molecular compounds mainly produced by red tide algae. In recent years, with the aggravation of environmental pollution, human deaths due to accidental ingestion of toxic shellfish are also often reported [18][19][20][21]. At present, the main methods for the detection of marine toxins include mouse bioassay (MBA) [22], enzyme-linked immunosorbent assay (ELISA) [23][24][25], high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS/MS), etc. [26][27][28]. However, these traditional methods have some drawbacks including the requirement of a technician, poor repeatability, expensive equipment, and issues related to animal ethics [22]. As a new detection technology, the biosensor is a kind of analysis systems using cell molecules and other bio-materials as sensitive elements, combined with a secondary sensor to detect a variety of chemicals by cascade amplified signal [29]. Because of their advantages of simple operation, high speed, high sensitivity, miniaturization, and easy automation, biosensors have been widely used in different fields including cell physiology [30], drug screening [31,32], food safety detection [33], disease diagnosis [34], etc.Aptamer, meaning "to fit", is a kind of oligonucleotide which can bind to target molecules with high selectivity and affinity. On account of the existence of aptamers in nature and the po...

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“…The main groups affecting aquatic systems are mycotoxins, algal, bacterial, and plant toxins [75]. Among them, the most relevant toxin groups found in freshwater are anatoxin-A (ATX), cylindrospermopsin (CYN), and microcystins (MCs); while marine toxins are classified into brevetoxin (BTX), okadaic acid (OA), palytoxin (PTX), saxitoxin (STX), cyclic imine (CI), domoic acid (DA), pectenotoxin (PTX), yessotoxin (YTX), azaspiracid (AZA), and ciguatoxin (CTX) [76]. According to the European Union legislation, the official reference method for the detection of paralytic shellfish toxins (PST) is liquid chromatography with fluorimetric detection (FLD) [77].…”

Section: Toxinsmentioning

confidence: 99%

Heavy Metal/Toxins Detection Using Electronic Tongues

2019

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The growing concern for sustainability and environmental preservation has increased the demand for reliable, fast response, and low-cost devices to monitor the existence of heavy metals and toxins in water resources. An electronic tongue (e-tongue) is a multisensory array mostly based on electroanalytical methods and multivariate statistical techniques to facilitate information visualization in a qualitative and/or quantitative way. E-tongues are promising analytical devices having simple operation, fast response, low cost, easy integration with other systems (microfluidic, optical, etc) to enable miniaturization and provide a high sensitivity for measurements in complex liquid media, providing an interesting alternative to address many of the existing environmental monitoring challenges, specifically relevant emerging pollutants such as heavy metals and toxins.

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Aptamer-Based Biosensors to Detect Aquatic Phycotoxins and Cyanotoxins

Cited by 67 publications

References 106 publications

Sensors, Biosensors, and Analytical Technologies for Aquaculture Water Quality

Sensors, Biosensors, and Analytical Technologies for Aquaculture Water Quality

Marine Toxins Detection by Biosensors Based on Aptamers

Heavy Metal/Toxins Detection Using Electronic Tongues

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