Aptamer-based approaches for the detection of waterborne pathogens

Vishwakarma, Archana; Lal, Roshni; Ramya, Mohandass

doi:10.1007/s10123-020-00154-w

Cited by 17 publications

(10 citation statements)

References 147 publications

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“…Impedimetric immunosensors for pathogen detection have been developed for their ease of use and low cost compared to other techniques such as colony-based detection, ELISA, or PCR. , Several types of sensors are possible, but those incorporating biorecognition are the most sensitive. They use biological recognition elements such as antibodies, small molecules such as antibiotics, or aptamers , grafted on an electrode to directly detect specific analytes. Independent of the type of sensor used, data analysis is based on the use of equivalent electrical circuits and more specifically on the Randles circuit.…”

Section: Examplesmentioning

confidence: 99%

Impedance Analysis of Electrochemical Systems

2022

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Interpretation of impedance spectroscopy data requires both a description of the chemistry and physics that govern the system and an assessment of the error structure of the measurement. The approach presented here includes use of graphical methods to guide model development, use of a measurement model analysis to assess the presence of stochastic and bias errors, and a systematic development of interpretation models in terms of the proposed reaction mechanism and physical description. Application to corrosion, batteries, and biological systems is discussed, and emerging trends in interpretation and implementation of impedance spectroscopy are presented.

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Section: Examplesmentioning

confidence: 99%

Impedance Analysis of Electrochemical Systems

2022

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“…Pathogenic strains have been successfully detected by aptamer-NanoZyme-mediated sensing platforms (both electrochemical and colorimetric) [101]. However, the applicability of aptamer-based immunoassays is not as wide as antibodies due to complexity in the selection of highly-specific aptamers [102]. The production of target-specific aptamers via the systematic evolution of ligands by exponential enrichment (SELEX) and post-SELEX is a time-consuming process [103].…”

Section: Aptamer-based Immunoassaymentioning

confidence: 99%

“…Any significant charge changes born by pathogens can enhance the complexity of a SELEX process [104,105]. The integration of aptamer-based assays with microfluidics, lateral flow, and surface-fabrication techniques can offer multiplexing However, the applicability of aptamer-based immunoassays is not as wide as antibodies due to complexity in the selection of highly-specific aptamers [102]. The production of target-specific aptamers via the systematic evolution of ligands by exponential enrichment (SELEX) and post-SELEX is a time-consuming process [103].…”

Section: Aptamer-based Immunoassaymentioning

confidence: 99%

Current Scenario of Pathogen Detection Techniques in Agro-Food Sector

Nehra

Kumar

et al. 2022

Biosensors

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Over the past-decade, agricultural products (such as vegetables and fruits) have been reported as the major vehicles for foodborne diseases, which are limiting food resources. The spread of infectious diseases due to foodborne pathogens poses a global threat to human health and the economy. The accurate and timely detection of infectious disease and of causative pathogens is crucial in the prevention and treatment of disease. Negligence in the detection of pathogenic substances can be catastrophic and lead to a pandemic. Despite the revolution in health diagnostics, much attention has been paid to the agro-food sector regarding the detection of food contaminants (such as pathogens). The conventional analytical techniques for pathogen detection are reliable and still in operation. However, laborious procedures and time-consuming detection via these approaches emphasize the need for simple, easy-to-use, and affordable detection techniques. The rapid detection of pathogens from food is essential to avoid the morbidity and mortality originating from the suboptimal nature of empiric pathogen treatment. This review critically discusses both the conventional and emerging bio-molecular approaches for pathogen detection in agro-food.

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“…Aptamer has been an attractive tool for various applications compared with antibodies and enzymes due to its cost-effectiveness, strong specificity, suitable pretreatment, simple operation, and short detection time [ 32 ]. Recently, aptasensors have been applied to cell imaging [ 33 , 34 ], drug delivery [ 35 , 36 ], disease treatment [ 37 , 38 ], microbial detection [ 39 , 40 ], and other fields. Hence, a smartphone-based aptasensor will be an ideal tool for POCT and analysis to obtain a fast and precise response in various fields.…”

Section: Introductionmentioning

confidence: 99%

Applications of Smartphone-Based Aptasensor for Diverse Targets Detection

Tan

et al. 2022

Biosensors

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Aptamers are a particular class of functional recognition ligands with high specificity and affinity to their targets. As the candidate recognition layer of biosensors, aptamers can be used to sense biomolecules. Aptasensors, aptamer-based biosensors, have been demonstrated to be specific, sensitive, and cost-effective. Furthermore, smartphone-based devices have shown their advantages in binding to aptasensors for point-of-care testing (POCT), which offers an immediate or spontaneous responding time for biological testing. This review describes smartphone-based aptasensors to detect various targets such as metal ions, nucleic acids, proteins, and cells. Additionally, the focus is also on aptasensors-related technologies and configurations.

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Aptamer-based approaches for the detection of waterborne pathogens

Cited by 17 publications

References 147 publications

Impedance Analysis of Electrochemical Systems

Impedance Analysis of Electrochemical Systems

Current Scenario of Pathogen Detection Techniques in Agro-Food Sector

Applications of Smartphone-Based Aptasensor for Diverse Targets Detection

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