Aptamer-Based Alternatives to the Conventional Immobilized Metal Affinity Chromatography for Purification of His-Tagged Proteins

Aptamer Loaded Superparamagnetic Beads For Selective Capturing And Gentle Release of Activated Protein C

“…A low yield of coupling (≤ 20%) was also described previously by Liu et al, where the coupling ratio of aptamer immobilization on CNBr-activated Sepharose beads was only 17% 21 . In addition, Lim et al described the e ciency of immobilization of His-tag-speci c aptamer on aminomethylated polystyrene resin as 510 fmol/mg bead 38 . One possible explanation is that the acidic character of aptamers, due to the ionizable phosphate groups and their negative net charge, likely produce charges repulsion between the aptamer and COOH-modi ed solid sorbent.…”

Covalent immobilization of coagulation factor VIII on magnetic nanoparticles for aptamer development

“…44,45 Considering the importance of aptamers as a selective affinity ligands in analytical processes and protein purification methods, numerous investigations have focused on selection process improvement. [46][47][48][49] In this regard, MNPs have a special place in aptamer technology. 22,50,51 Accordingly, we evaluated the immobilization of FVIII on MNPs for application in the aptamer selection process.…”

Affinity chromatography: A review of trends and developments over the past 50 years

“…Metal ions such as Cu + , Ag + , Pd 2+ , Pt 2+ , Cd 2+ , and Hg 2+ tend to bind targets that contain sulfur, while metal ions such as Ni 2+ , Cu 2+ , and Zn 2+ tend to coordinate with targets having accessible groups that contain nitrogen, sulfur, or oxygen [131,[279][280][281]. Protein purification is a common use of IMAC [131,282,283]. For instance, this method has been employed in purifying natural proteins such as HSA, immunoglobulins, lysozyme, and αamylase [129][130][131]276,283].…”

“…For instance, this method has been employed in purifying natural proteins such as HSA, immunoglobulins, lysozyme, and αamylase [129][130][131]276,283]. IMAC has also been used to isolate histidine-tagged recombinant proteins, DNA-based aptamers, and phosphopeptides [131,278,282,284,285]. Boronic acid and its derivatives represent another class of non-biological binding agents that have been utilized in affinity chromatography [286][287][288][289].…”