Aptamer-assembled nanomaterials for fluorescent sensing and imaging

Lu, Danqing; He, Lei; Zhang, Ge; Ai-ping, LV; Wang, Ruowen; Zhang, Xiaobing; Tan, Weihong

doi:10.1515/nanoph-2015-0145

Cited by 48 publications

(30 citation statements)

References 86 publications

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“…Nitrogen‐doped GQDs (N‐GQDs) are also known to show a strong two‐photon absorption cross‐section and are also found to be an efficient two‐photon fluorescent probe for TPFI . All the more strikingly, graphene and its derivatives additionally show awesome potential as productive quenchers in graphene‐based nanosensors for some fluorescent moieties, including molecular dyes, QDs, and conjugated polymers by means of FL resonance energy exchange or charge exchange . Moreover, the inherent Raman signs of GOs, G line around 1600 cm −1 and the D line (the symmetry A1g mode) around 1350 cm −1 , can be additionally upgraded by metal nanoparticles (Au or Ag) and have been used in Raman imaging for SERS application …”

Section: Biomedical Applicationsmentioning

confidence: 99%

“…Transferrin functionalized GO–PEG with solid two‐photon radiance as a nonfading optical test for 3D TPFI and laser‐based disease miniaturized scale surgery, utilizing a ultrafast beat laser as the excitation source has been reported . From that point onward, N‐GQDs have been used as a proficient two‐photon fluorescent tests for cell and profound tissue imaging . In N‐GQDs, dimethylformamide with a normal size of ≈3 nm was used as both solvent and nitrogen source and here the solvothermal procedure was used for synthesis.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

Graphene and Graphene‐Based Materials in Biomedical Science

Swetha

Manisha

Prasad

2018

Part & Part Syst Charact

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Graphene and its composite materials are very important in many disciplines of science and have been used enormously by researchers since their discovery in 2004. These are a new group of compounds, and are also wonderful model systems for quantum behavior studies. Their properties like exceptional conductivity, biocompatibility, surface area, mechanical strength, and thermal properties make them rising stars in the scientific community. Graphene and its composite compounds are utilized widely in different medical applications, for example, biosensing of biological compounds responsible for disease development, bioimaging of various cells, tissues, microorganisms, animal models, etc. In addition, they are used for enhancing and supporting the stem cell differentiation, i.e., regenerative medicine for regeneration studies of various human organs, tissue engineering in biology for the development of carrier materials, as well as in bone reformation. This review focuses on the modification procedure involved in the fabrication of graphene‐based biomaterials for various applications and recent developments in research related to graphene and graphene‐based materials in biosensing, optical sensing, gas sensing, drug, gene, protein delivery, tissue engineering, and bioimaging. In addition, the potential toxicological effects of graphene‐based biomaterials are discussed.

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Section: Biomedical Applicationsmentioning

confidence: 99%

Section: Biomedical Applicationsmentioning

confidence: 99%

Graphene and Graphene‐Based Materials in Biomedical Science

Swetha

Manisha

Prasad

2018

Part & Part Syst Charact

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“…The thrombin targeting DNA aptamer acted as a nucleation and capping reagent for QDs' synthesis, while the aptamer retained its structure during the capping process. Once the aptamer binds to the protein target, the QD's fluorescence quenches, resulting in the optical reporting of specific protein recognition . Furthermore, a class of aptamers for targeting cancer cells can be used as templates for synthesizing and stabilizing nanocrystals .…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Programmable and Multifunctional DNA‐Based Materials for Biomedical Applications

et al. 2018

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DNA encodes the genetic information; recently, it has also become a key player in material science. Given the specific Watson-Crick base-pairing interactions between only four types of nucleotides, well-designed DNA self-assembly can be programmable and predictable. Stem-loops, sticky ends, Holliday junctions, DNA tiles, and lattices are typical motifs for forming DNA-based structures. The oligonucleotides experience thermal annealing in a near-neutral buffer containing a divalent cation (usually Mg ) to produce a variety of DNA nanostructures. These structures not only show beautiful landscape, but can also be endowed with multifaceted functionalities. This Review begins with the fundamental characterization and evolutionary trajectory of DNA-based artificial structures, but concentrates on their biomedical applications. The coverage spans from controlled drug delivery to high therapeutic profile and accurate diagnosis. A variety of DNA-based materials, including aptamers, hydrogels, origamis, and tetrahedrons, are widely utilized in different biomedical fields. In addition, to achieve better performance and functionality, material hybridization is widely witnessed, and DNA nanostructure modification is also discussed. Although there are impressive advances and high expectations, the development of DNA-based structures/technologies is still hindered by several commonly recognized challenges, such as nuclease instability, lack of pharmacokinetics data, and relatively high synthesis cost.

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“…Second, for intracellular or in vivo imaging, rationally designed FNA nanomaterials can greatly increase the sensitivity and specificity for biomolecular recognition because of the well‐controlled probe density, orientation, and surface passivation . In this way, these FNA nanomaterials could provide a versatile and efficient platform for imaging of various targets in vivo.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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equally exciting and perhaps more challenging field of application is toward their in vivo applications, including living animals and human bodies, in diverse areas, [2] such as sensing, drug delivery, medical imaging, and cancer therapy. However, most of these nanomaterials lack selectivity toward targets of interests. Therefore, to employ these nanomaterials for a wider range of in vivo applications, various molecular engineering approaches have been employed to obtained nanomaterials functionalized with biomolecules to enable selective targeting. [3][4][5] Among these biomolecules, functional nucleic acids, or FNAs, have shown the most promise.FNAs are DNA or RNA molecules that can interact with or bind to a specific analyte, resulting in a conformational change and/or a catalytic reaction. [6] As their names suggested, ribozymes and deoxyribozymes (called DNAzyme hereafter) can act as enzymes in the presence of cofactors to catalyze various reactions, including cleavage and ligation of RNA/DNA, and phosphorylation and peroxidation of DNA. On the other hand, riboswitches and DNA aptamers are antibody-like receptors that can bind target molecules with high affinity and selectivity. Furthermore, the binding abilities of riboswitches and aptamers can be combined with the enzymatic function of ribozymes or DNAzymes to form aptazymes so that the binding of targets can inhibit or enhance the catalytic activities. These nucleic acids, including DNAzymes, aptamers, and aptazymes, are collectively known as FNAs to emphasize their functions beyond the traditional genetic storage and transformations roles for DNA and RNA. Unlike DNA and RNA inside biological systems, FNAs are isolated via a combinatorial technique known as in vitro selection, or a process also known as systematic evolution of ligands by exponential enrichment (SELEX). The discovery of new functions of nucleic acids has not only resulted in major advances in the fields of molecular biology and biochemistry, but also revolutionized sensors designs for both in vitro and in vivo applications. [7,8] Over the past decade, numerous FNA-based nanomaterials have been developed. [9][10][11][12] Among these FNA-based nanosystems, the functions of FNAs can be categorized into two types: diagnostic function and therapeutic function. For the diagnostic function, the FNAs serve either as the biorecognition ligands for direct sensing and imaging of the Recent advances in nanotechnology and engineering have generated many nanomaterials with unique physical and chemical properties. Over the past decade, numerous nanomaterials are introduced into many research areas, such as sensors for environmental monitoring, food safety, point-ofcare diagnostics, and as transducers for solar energy transfer. Meanwhile, functional nucleic acids (FNAs), including nucleic acid enzymes, aptamers, and aptazymes, have attracted major attention from the biomedical community due to their unique target recognition and catalytic properties. Benefiting from the recent progress of molecular engine...

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Aptamer-assembled nanomaterials for fluorescent sensing and imaging

Cited by 48 publications

References 86 publications

Graphene and Graphene‐Based Materials in Biomedical Science

Graphene and Graphene‐Based Materials in Biomedical Science

Programmable and Multifunctional DNA‐Based Materials for Biomedical Applications

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

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