Approaching a Million-Fold Sensitivity Increase in Capillary Electrophoresis with Direct Ultraviolet Detection:  Cation-Selective Exhaustive Injection and Sweeping

Quirino, Joselito P.; Terabe, Shigeru

doi:10.1021/ac990344b

Cited by 316 publications

(254 citation statements)

References 27 publications

(51 reference statements)

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“…In these conditions, approximately 4.6 nL of sample solution (assumed viscosity of 1 cP) is introduced into the capillary during the injection. SEF values achieved by the integration of an FESI preconcentration step were then calculated as follows for six different proteins [35]: SEF ¼ peak parameters obtained withpreconcentrain peak parameters obtained without preconcentration…”

Section: Sensitivity and Detection Limitmentioning

confidence: 99%

High‐sensitive protein analysis by FESI‐CE‐MALDI‐MS

2011

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Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are two techniques highly suitable for the separation and detection of intact proteins. Herein, based on the use of a recently introduced iontophoretic fraction collection interface for the coupling of CE and MALDI-MS, the potential of the combination of both techniques for the analysis of intact proteins is assessed. To further provide a bioanalytical platform with high-sensitivity capabilities, field-enhanced sample injection is integrated as on online preconcentration strategy upstream from the electrokinetic separation. Under optimized conditions, more than 3200-and 4800-fold improvement, respectively in terms of peak height and peak area, as well as LODs ranging from 5 to 10 nM, has been achieved.

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Section: Sensitivity and Detection Limitmentioning

confidence: 99%

High‐sensitive protein analysis by FESI‐CE‐MALDI‐MS

2011

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“…However, some of the described methodologies may be used in combination to reach higher SEFs. For example, FESI has been successfully combined with sweeping a few years ago [19]. In that case, extremely high SEFs (between 550,000 and 900,000) have been obtained for 1-naphthylamine and laudanosine, respectively.…”

Section: Introductionmentioning

confidence: 99%

Integration of various stacking processes in carrier ampholyte-based capillary electrophoresis

Busnel

Saux

Descroix

et al. 2008

Journal of Chromatography A

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Field-enhanced sample stacking, field-enhanced sample injection as well as electrokinetic supercharging have been successfully integrated in carrier ampholyte-based capillary electrophoresis. Through the analysis of different test sample mixtures, it has been shown that the carrier ampholyte-based background electrolytes, in spite of their very low conductivity, allow efficient online preconcentration of analytes by fieldamplified techniques. Sensitivity enhancement factors of the same magnitude as those usually encountered with classical conductive background electrolytes have been obtained in such carrier ampholyte-based buffers. Depending on the online preconcentration method that has been integrated, sensitivity enhancement factors between 50 and several thousands have been reached.

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“…Sweeping is representative of this group [5]. Among them, sample stacking and sweeping are most frequently used in MEKC [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

“…Sweeping is defined as a phenomenon where analytes are picked up and accumulated by the pseudostationary phase (micelles [5,[19][20][21][22] or other complexation additives [23,24]) that penetrates the sample zone. It occurs when the sample matrix is void of charged carrier phase, and the sample matrix may have a higher, similar, or lower conductivity compared with the BGE [19][20][21][22][23]31]. Concentration factors of up to 5000 have been reported for some neutral analytes by sweeping long sample zones with SDS in CE [5].…”

Section: Introductionmentioning

confidence: 99%

Pressure‐assisted field‐amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC

Fang¹,

Yang²,

Sun³

et al. 2007

Electrophoresis

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This paper describes a novel method that applies pressure-assisted field-amplified sample injection with reverse migrating micelles (PA-FASI-RMM) for the online concentration of neutral analytes in MEKC with a low-pH BGE. After injection of a plug of water into the separation capillary, negative voltage and positive pressure were simultaneously applied to initialize PA-FASI-RMM injection. The hydrodynamic flow generated by the positive pressure compensated the reverse EOF in the water plug and allowed the water plug to remain in the capillary during FASI with reverse migrating micelles (FASI-RMM) to obtain a much longer injection time than usual, which improved stacking efficiency greatly. Equations describing this injection mode were introduced and were supported by experimental results. For a 450-s online PA-FASI-RMM injection, three orders of magnitude sample enhancement in terms of peak area could be observed for the steroids and an achievement of detection limits was between 1 and 10 ng/mL.

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Approaching a Million-Fold Sensitivity Increase in Capillary Electrophoresis with Direct Ultraviolet Detection: Cation-Selective Exhaustive Injection and Sweeping

Cited by 316 publications

References 27 publications

High‐sensitive protein analysis by FESI‐CE‐MALDI‐MS

High‐sensitive protein analysis by FESI‐CE‐MALDI‐MS

Integration of various stacking processes in carrier ampholyte-based capillary electrophoresis

Pressure‐assisted field‐amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC

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