Approaches to characterise chromatographic column performance based on global parameters accounting for peak broadening and skewness

Baeza-Baeza, J.J.; Pous-Torres, S.; Torres-Lapası́o, J.R.; García-Álvarez-Coque, M.C.

doi:10.1016/j.chroma.2010.02.010

Cited by 24 publications

(15 citation statements)

References 21 publications

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“…Our results proved that characterization of monolithic columns is highly affected by peak asymmetry, which is influenced by extra‐column contributions but also by the own nature of the column chemistry. Furthermore, these findings are in agreement with previous results in which different set of compounds were assayed, such as, diuretics and beta‐blockers , phenols , betamethasone‐17‐valerate and betamethasone‐enolaldehyde , weak and strong bases such as pyridine, quinine, nortriptyline, aniline and 4‐ethylaniline and benzene , where monolithic columns showed higher factor asymmetry compared to packed columns.…”

Section: Resultssupporting

confidence: 91%

RpeakChrom: Novel R package for the automated characterization and optimization of column efficiency in high‐performance liquid chromatography analysis

et al. 2017

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Characterization of chromatographic columns using the traditional van Deemter method is limited by the necessity of calculating extra-column variance, issue particularly relevant when modeling asymmetrical peaks eluted from monolithic columns. A novel R package that implements Parabolic Variance Modified Gaussian approach for accurate peak modeling, van Deemter equation and two alternatives approaches, based on van Deemter, has been developed to calculate the height equivalent to a theoretical plate (HETP). To assess package capabilities conventional packed reverse-phase and monolithic HPLC columns were characterized. Peaks eluted from the monolithic column showed a high value of factor asymmetry due, in part, to the contribution of extra-column factors. Such deviation can be circumvented by the two alternatives approaches implemented in the R-package. Furthermore, increased values of eddy diffusion and mass transfer kinetics terms in HETP were observed for the packed column, while accuracy was below 9% in all cases. These results showed the usefulness of the R-package for both modeling chromatographic peaks and assessing column efficiency. The RpeakChrom package could become a helpful tool for testing new stationary phases during column development and to evaluate column during its lifetime. This R tool is freely available from CRAN (https://CRAN.R-project.org/package=RpeakChrom).

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Section: Resultssupporting

confidence: 91%

RpeakChrom: Novel R package for the automated characterization and optimization of column efficiency in high‐performance liquid chromatography analysis

et al. 2017

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“…These are constant, whereas the peak width yielded inside the column increases with the retention time [28]. Thus, the peaks of the least retained solutes are narrower, but for this reason, they are more strongly affected by the extra-column peak broadening.…”

Section: Efficiency and Asymmetry Changes With Mobile Phase Compositionmentioning

confidence: 94%

Comparison of the performance of non‐ionic and anionic surfactants as mobile phase additives in the RPLC analysis of basic drugs

Ruiz-Ángel¹,

García-Álvarez-Coque²

2011

J of Separation Science

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Surfactants added to the mobile phases in reversed-phase liquid chromatography (RPLC) give rise to a modified stationary phase, due to the adsorption of surfactant monomers. Depending on the surfactant nature (ionic or non-ionic), the coated stationary phase can exhibit a positive net charge, or just change its polarity remaining neutral. Also, micelles in the mobile phase introduce new sites for solute interaction. This affects the chromatographic behavior, especially in the case of basic compounds. Two surfactants of different nature, the non-ionic Brij-35 and the anionic sodium dodecyl sulfate (SDS) added to water or aqueous-organic mixtures, are here compared in the separation of basic compounds (β-blockers and tricyclic antidepressants). The reversible/irreversible adsorption of the monomers of both surfactants on the stationary phase was examined. The changes in the nature of the chromatographic system using different columns and chromatographic conditions were followed based on the changes in retention and peak shape. The study revealed that Brij-35 is suitable for analyzing basic compounds of intermediate polarity, using "green chemistry", since the addition of an organic solvent is not needed and Brij-35 is a biodegradable surfactant. In contrast, RPLC with hydro-organic mixtures or mobile phases containing SDS required high concentrations of organic solvents.

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“…That is, at a flow rate of 1 mL/min there appeared to be, in the worst case, an equivalent of up to ∼20 L of extra-column volume that could not be accounted for (Table 3, column 4). At a flow rate of 2 mL/min this discrepancy further increased to ∼30-35 L. It is important to recognize that the absolute values of these differences are dependent on how the actual extra-column peak variance is determined [41,42]. In this case, 2 ec was calculated using obtained from the 5 peak width (see Section 2.1).…”

Section: Determination Of Extra-column Dispersion Using the "No-colummentioning

confidence: 98%

“…The rationale for doing this is as follows. Although exact numerical integration of the peak (statistical moments) is considered the most accurate method, it can overestimate the variance due to errors arising from the integration limits, the baseline drift and noise [41,42]. In addition, it is more difficult to relate this value to the plate number of a peak obtained when the column is present (N obs ), because column efficiencies are usually calculated from the actual peak widths measured at specific points on the peak (either at 1/2 height, or 4 , or 5 ).…”

Section: Determination Of Extra-column Dispersion Using the "No-colummentioning

confidence: 99%

Modifying conventional high-performance liquid chromatography systems to achieve fast separations with Fused-Core columns: A case study

Alexander

Waeghe²,

Himes

et al. 2011

Journal of Chromatography A

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Approaches to characterise chromatographic column performance based on global parameters accounting for peak broadening and skewness

Cited by 24 publications

References 21 publications

RpeakChrom: Novel R package for the automated characterization and optimization of column efficiency in high‐performance liquid chromatography analysis

RpeakChrom: Novel R package for the automated characterization and optimization of column efficiency in high‐performance liquid chromatography analysis

Comparison of the performance of non‐ionic and anionic surfactants as mobile phase additives in the RPLC analysis of basic drugs

Modifying conventional high-performance liquid chromatography systems to achieve fast separations with Fused-Core columns: A case study

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