Approaches for in vitro propagation and production of plumbagin in Plumbago spp.

Pandey, Devendra Kumar; Katoch, Kajal; Das, Tuyelee; Majumder, Madhumita; Dhama, Kuldeep; Mane, Abhijit Bhagwan; Gopalakrishnan, Abilash Valsala; Dey, Abhijit

doi:10.1007/s00253-023-12511-6

Cited by 3 publications

(1 citation statement)

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“…It has been previously documented that plumbagin production can be enhanced, particularly in the genus Plumbago . Numerous Plumbago culture types, including root, hairy root, callus and cell suspension cultures, have been examined [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 40 ], but the in vitro-regenerated shoots of P. indica have not been the subject of this kind of investigation. One advantage of application with the regenerated shoots is maintaining genetic stability.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Plumbagin Production through Elicitation in In Vitro-Regenerated Shoots of Plumbago indica L.

Jirakiattikul,

Ruangnoo,

Sangmukdee

et al. 2024

Plants

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Plumbago indica L. contains a valuable bioactive compound called plumbagin. Elicited regenerated shoots grown in vitro could be another source of high-yielding plumbagin. The purpose of this investigation was to examine the effects of elicitor type and concentration, as well as elicitation period, on plumbagin content in in vitro-regenerated shoots of P. indica. Nodal explants were cultured on Murashige and Skoog (MS) medium containing 1 mg/L benzyladenine (BA) in combination with 0–150 mg/L yeast extract or 50–150 µM salicylic acid for four weeks. Plumbagin levels of 3.88 ± 0.38% and 3.81 ± 0.37% w/w g dry extract were achieved from the 50 and 100 mg/L yeast extract-elicited shoots, which were higher than the value obtained for the control. However, the addition of salicylic acid did not increase the plumbagin content. In the elicitation period experiment, nodal explants were cultured on MS medium supplemented with 1 mg/L BA and 50 mg/L yeast extract for durations of three, four and five weeks. The 4-week yeast extract-elicited shoot had a maximum plumbagin content of 3.22 ± 0.12% w/w g dry extract, greater than that of the control. In summary, the plumbagin content of the in vitro P. indica shoots was enhanced by 4-week elicitation using 50 mg/L yeast extract.

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