Approaches for In Vitro Conservation of Woody Plants Germplasm

González-Arnao, María Teresa; Dolce, Natalia Raquel; González‐Benito, M. Elena; Martinez, Carlos Roman Castillo; Cruz‐Cruz, Carlos A.

doi:10.1007/978-3-319-66426-2_13

Cited by 7 publications

(1 citation statement)

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“…Although maintenance of valuable genotypes in field collections is the most common way to preserve woody plants, the application of in vitro techniques has also been proposed [ 38 ]. Responses to cold storage of in vitro cultures of plant species and varieties can be highly varied, and even if they can survive the storage, differences in their regrowth capacity are common.…”

Section: Discussionmentioning

confidence: 99%

Response of Rowan Berry (Sorbus redliana) Shoot Culture to Slow Growth Storage Conditions

Mendler-Drienyovszki

Magyar-Tábori

2023

Plants

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Slow growth storage can preserve the genetic resources of endangered species such as those of genus Sorbus. Our aim was to study the storability of rowan berry in vitro cultures, their morpho-physiological changes, and regeneration ability after different storage conditions (4 ± 0.5 °C, dark; and 22 ± 2 °C, 16/8 h light/dark). The cold storage lasted for 52 weeks, and observations were made every four weeks. Cultures showed 100% survival under cold storage, and those taken from the storage showed 100% regeneration capacity after the passages. A dormancy period lasting about 20 weeks was observed, followed by intensive shoot growth until the 48th week, which led to the exhaustion of the cultures. The changes could be traced to the reduction of the chlorophyll content and the Fv/Fm value, as well as in the discoloration of the lower leaves and the appearance of necrotic tissues. Long, etiolated shoots (89.3 mm) were obtained at the end of cold storage. Shoot cultures stored in a growth chamber as control (22 ± 2 °C, 16/8 h light/dark) senesced and died after 16 weeks. Explants from stored shoots were subcultured for four weeks. The number and length of newly developed shoots were significantly higher on explants from cold storage compared to those from control cultures if the storage was longer than one week.

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Section: Discussionmentioning

confidence: 99%