Approaches for evaluation of novel CPP-based cargo delivery systems

Abstract

Cell penetrating peptides (CPPs) can be broadly defined as relatively short synthetic, protein derived or chimeric peptides. Their most remarkable property is their ability to cross cell barriers and facilitate the translocation of cargo, such as drugs, nucleic acids, peptides, small molecules, dyes, and many others across the plasma membrane. Over the years there have been several approaches used, adapted, and developed for the evaluation of CPP efficacies as delivery systems, with the fluorophore attachment as the most widely used approach. It has become progressively evident, that the evaluation method, in order to lead to successful outcome, should concede with the specialties of the delivery. For characterization and assessment of CPP-cargo a combination of research tools of chemistry, physics, molecular biology, engineering, and other fields have been applied. In this review, we summarize the diverse, in silico, in vitro and in vivo approaches used for evaluation and characterization of CPP-based cargo delivery systems.

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“…To overcome this problem, several tools, such as cell-penetrating peptides (CPPs), have been developed. CPPs are short peptides of 5 to 35 amino acids that are able to penetrate the plasma membrane and deliver various cargos ranging from nanoparticles to macromolecules (e.g., siRNAs, DNAs, proteins, peptides, antibodies, and therapeutic agents). − CPPs are classified into three categories: cationic, amphipathic, and hydrophobic . Cationic CPPs are generally arginine-rich peptides and are the most studied class of CPPs.…”