Approaches for Enhancing Extraction of Bacterial Polyhydroxyalkanoates for Industrial Applications

Macagnan, Karine Laste; Alves, Mariane Igansi; Moreira, Angelita da Silveira

doi:10.1007/978-981-13-3759-8_15

Cited by 7 publications

(4 citation statements)

References 56 publications

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“…The production of bioplastic from microalgae has still not been developed at commercial scale since the overall economical balance is still higher than that of traditional plastics and other polymeric materials. The cost associated with the extraction and purification of the biopolymer must be considered, as it can represent up to 50% of the total process cost [ 95 ]. The environmental impact of the reagents used is also of importance.…”

Section: Discussionmentioning

confidence: 99%

Accumulation of PHA in the Microalgae Scenedesmus sp. under Nutrient-Deficient Conditions

et al. 2020

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Traditional plastics have undoubted utility and convenience for everyday life; but when they are derived from petroleum and are non-biodegradable, they contribute to two major crises today’s world is facing: fossil resources depletion and environmental degradation. Polyhydroxyalkanoates are a promising alternative to replace them, being biodegradable and suitable for a wide variety of applications. This biopolymer accumulates as energy and carbon storage material in various microorganisms, including microalgae. This study investigated the influence of glucose, N, P, Fe, and salinity over the production of polyhydroxyalkanoate (PHA) by Scenedesmus sp., a freshwater microalga strain not previously explored for this purpose. To assess the effect of the variables, a fractional Taguchi experimental design involving 16 experimental runs was planned and executed. Biopolymer was obtained in all the experiments in a wide range of concentrations (0.83–29.92%, w/w DW), and identified as polyhydroxybutyrate (PHB) by FTIR analysis. The statistical analysis of the response was carried out using Minitab 16, where phosphorus, glucose, and iron were identified as significant factors, together with the P-Fe and glucose-N interactions. The presence of other relevant macromolecules was also quantified. Doing this, this work contributes to the understanding of the critical factors that control PHA production and present Scenedesmus sp. as a promising species to produce bio-resources in commercial systems.

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Section: Discussionmentioning

confidence: 99%

Accumulation of PHA in the Microalgae Scenedesmus sp. under Nutrient-Deficient Conditions

et al. 2020

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“…The latter is an attractive procedure widely investigated, as evidenced by the great number of scientific papers, reviews or patents on this topic, mainly dealing with PHAs from pure culture [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. In alternative to chlorinated hydrocarbons, which are the best solvents for PHAs, many research studies address the use of green solvents, with low or no toxicity and possibly derived from biochemical conversion, to overcome ecological issues, limitations involving worker safety or stringent regulations on solvent traces in goods for particular applications [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Ethylic Esters as Green Solvents for the Extraction of Intracellular Polyhydroxyalkanoates Produced by Mixed Microbial Culture

et al. 2021

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Volatile fatty acids obtained from the fermentation of the organic fraction of municipal solid waste can be used as raw materials for non-toxic ethyl ester (EE) synthesis as well as feedstock for the production of polyhydroxyalkanoates (PHAs). Taking advantage of the concept of an integrated process of a bio-refinery, in the present paper, a systematic investigation on the extraction of intracellular poly(3-hydroxybutyrate-co-3-hydroxyvalerate), produced by mixed microbial culture by using EEs was reported. Among the tested EEs, ethyl acetate (EA) was the best solvent, dissolving the copolymer at the lowest temperature. Then, extraction experiments were carried out by EA at different temperatures on two biomass samples containing PHAs with different average molecular weights. The parallel characterization of the extracted and non-extracted PHAs evidenced that at the lower temperature (100 °C) EA solubilizes preferentially the polymer fractions richer in 3HV comonomers and with the lower molecular weight. By increasing the extraction temperature from 100 °C to 125 °C, an increase of recovery from about 50 to 80 wt% and a molecular weight reduction from 48% to 65% was observed. The results highlighted that the extracted polymer purity is always above 90 wt% and that it is possible to choose the proper extraction condition to maximize the recovery yield at the expense of polymer fractionation and degradation at high temperatures or use milder conditions to maintain the original properties of a polymer.

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“…By overexpressing the genes minC and minD in various phaP deprived strains of H. bluephagenesis TD01, the cell size of this bacterium was drastically enlarged allowing it to accommodate PHA granules of up to 10 μm. Besides, PHAs of higher molecular weight are easy to recover by centrifugation, filtration or sedimentation (Macagnan et al, 2019 ). Intense efforts have been invested in PHA granule enlargement to improve recovery (Cai et al, 2012 ; De Almeida et al, 2007 ; Melanie et al, 2018 ).…”

Section: Cofactor and Morphology Engineering Strategiesmentioning

confidence: 99%

Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery

Acuña

Poblete‐Castro

2022

Microbial Biotechnology

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Microbial production of biopolymers derived from renewable substrates and waste streams reduces our heavy reliance on petrochemical plastics. One of the most important biodegradable polymers is the family of polyhydroxyalkanoates (PHAs), naturally occurring intracellular polyoxoesters produced for decades by bacterial fermentation of sugars and fatty acids at the industrial scale. Despite the advances, PHA production still suffers from heavy costs associated with carbon substrates and downstream processing to recover the intracellular product, thus restricting market positioning. In recent years, model‐aided metabolic engineering and novel synthetic biology approaches have spurred our understanding of carbon flux partitioning through competing pathways and cellular resource allocation during PHA synthesis, enabling the rational design of superior biopolymer producers and programmable cellular lytic systems. This review describes these attempts to rationally engineering the cellular operation of several microbes to elevate PHA production on specific substrates and waste products. We also delve into genome reduction, morphology, and redox cofactor engineering to boost PHA biosynthesis. Besides, we critically evaluate engineered bacterial strains in various fermentation modes in terms of PHA productivity and the period required for product recovery.

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Approaches for Enhancing Extraction of Bacterial Polyhydroxyalkanoates for Industrial Applications

Cited by 7 publications

References 56 publications

Accumulation of PHA in the Microalgae Scenedesmus sp. under Nutrient-Deficient Conditions

Accumulation of PHA in the Microalgae Scenedesmus sp. under Nutrient-Deficient Conditions

Ethylic Esters as Green Solvents for the Extraction of Intracellular Polyhydroxyalkanoates Produced by Mixed Microbial Culture

Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery

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