Approach to New peach rootstocks resistant to root-knot nematodes (Meloidogyne species) selected from local Mit-Ghamer peach cultivar

Eliwa, Galal I.; Hagag, Eman S.

doi:10.1016/j.scienta.2021.110118

Cited by 8 publications

(12 citation statements)

References 15 publications

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“…Concerning the behavior of genotype rootstock as affected by BAP and IBA combinations on average shoot multiplication%, it was clear from the data presented in Table (6) that although the Garnem genotype recorded the highest value of average shoot multiplication% (63.78%), there was no signi cant difference between the Nemared and Okinawa genotypes, which recorded 61.47 and 57.61%, 57.61% respectively.…”

Section: Effect Of Bap With or Without Iba On Average Shoot Multiplic...mentioning

confidence: 93%

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Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocks a. In vitro Explant Surface Sterilization and Bud Proliferation

Eliwa,

El-Dengawy,

Gawish

et al. 2023

Preprint

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The present study was conducted in the Laboratory of Tissue Culture, Horticulture Department, Faculty of Agriculture, Damietta University, Egypt. The objective of this study was to establish a micropropagation protocol suitable for three imported peach rootstocks: Okinawa (P. persica), Nemared (P. persica × P. davidiana) × P. persica), and Garnem (P. dulcis × P. persica) in vitro. The results showed that soaking the explants in sodium hypochlorite (NaOCl) at 20% for 15 minutes produced the highest responsiveness (82.81%), survival (96.61%), with the lowest mortality (3.14%) and contamination (0.24%). Explants of the Garnem genotype had the best response (89.12%), survival (90.62%), lowest mortality (0.00%), and highest contamination (9.37%) when compared to the other genotypes. In comparison with axillary buds, the shoot tip displayed the highest responsiveness, survival, and death (100, 87.40, and 12.59%, respectively), as well as the least significant contamination (0.00%). Additionally, the percentages of responsive, survived, dead, and contaminated explants at the various collection dates varied significantly. The 6-benzylaminopurine (BAP) concentrations used (3 to 5.0 mg/L) demonstrated similar behavior in terms of in vitro proliferation, with rates of 3.77 to 6.11, 4.33 to 8.88, and 3.33 to 7.44 shoot numbers per explant for the Okinawa, Nemared, and Garnem peach rootstocks, respectively, indicating that the number of shoot proliferations is genotype-dependent. Additionally, using 5.0 mg/L BAP in combination with 0.2 mg/L IBA significantly increased average shoot proliferation (96.29%), number of shoots per explant (7.48), and average leaf number/explant (16.33) compared to the other treatments. Based on these results, adventitious bud development was enhanced during in vitro multiplication of the Okinawa, Nemared, and Garnem peach rootstocks by the synergistic interaction of indole-butyric acid (IBA) and 6-benzylaminopurine (BAP).

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Section: Effect Of Bap With or Without Iba On Average Shoot Multiplic...mentioning

confidence: 93%

“…The results for the average multiplication percentage of the Okinawa, Nemared, and Garnem peach rootstocks as affected by the plant growth regulators are shown in Table (6) and Fig. (5f).…”

Section: Effect Of Bap With or Without Iba On Average Shoot Multiplic...mentioning

confidence: 99%

Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocks a. In vitro Explant Surface Sterilization and Bud Proliferation

Eliwa,

El-Dengawy,

Gawish

et al. 2023

Preprint

Self Cite

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“…The chemical nematicides are the most commonly used management method but they are costly and contaminate the environment (Adegbite et al 2005). Hence, using eco-friendly methods like resistant rootstocks and biological control has attracted special attention recently (Eliwa and Hagag 2021). So, our country has imported peach rootstocks which resistant to root-knot nematodes e.g.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Explant Sterilization and Bud Proliferation Studies on Some Imported Peach Rootstocks

Eliwa,

El-Dengawy,

Gawish

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

The present investigation was conducted in the Laboratory of Tissue Culture, Horticulture Department, Faculty of Agriculture, Damietta University, Egypt. The objective of this study was to establish a micropropagation protocol suitable for three imported peach rootstocks {Okinawa (P. persica), Nemared (P. persica × P. davidiana) × P. persica) and Garnem (P. dulcis × P. persica)} In vitro. According to the results, soaking the explants in sodium hypochlorite (NaOCl) at 20% for 15 min achieved the highest value of responsive (82.81%) and survived (96.61%) with a minimum value of dead (3.14%) and contaminated (0.24%). Garnem genotype explants had achieved the best response (89.12%) and Survived (90.62%) with minimum dead (0.00%) and maximum contamination (9.37%). The shoot tips showed the highest responsive, Survived, and dead% (100, 87.40 and12.59% respectively) and minimum significant contaminated (0.00%) in comparison with axillary buds. Also, the explants differed significantly in their percentages of responsive, survived, dead, and contaminated at the different collected dates. The 6-benzylaminopurine (BAP) concentrations used (3 to 5.0 mg/L-1) demonstrated similar behavior in terms of in vitro proliferation, with rates of 3.77 to 6.11, 4.33 to 8.88 and 3.33 to 7.44 shoots per explant for Okinawa, Nemared, and Garnem peach rootstocks respectively, indicating that the number of shoots proliferation is genotype dependent. Moreover, using 5.0 mg/L-1 BAP in combination with 0.2 mg/L-1 IBA produced higher significant values of average shoot proliferation % (96.29%) as well as number of shoots per explant (7.48) and average Leaves number/explant (16.33) than the other treatments. From these results, we could conclude that indole-butyric acid (IBA) synergists with 6-benzylaminopurine (BAP) improved adventitious bud development in vitro multiplication of Okinawa, Nemared, and Garnem peach rootstocks.

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“…The chemical nematicides are the most commonly used management method but they are costly and contaminate the environment (Adegbite et al 2005). Hence, using eco-friendly methods like resistant rootstocks and biological control have attracted special attention recently (Eliwa and Hagag 2021). So, our country has imported peach rootstocks which resistant to root-knot nematodes e.g.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocksa. In vitro Explant Surface Sterilization and Bud Proliferation

Eliwa

2023

Preprint

Self Cite

View full text Add to dashboard Cite

This study was conducted in the Laboratory of Tissue culture, Horticulture Department, Faculty of Agriculture, Damietta University, Egypt. The objective of this study was to establish a micropropagation protocol suitable for three imported peach rootstocks {Okinawa (P. persica), Nemared (P. persica × P. davidiana) × P. persica) and Garnem (P. dulcis × P. persica)} In vitro. According to the results, soaking the explants in sodium hypochlorite (NaOCl) at 20% for 15 min achieved the highest value of responsive (82.81%) and Survived (96.61%) with minimum value of dead (3.14%) and contaminated (0.24%). Garnem genotype explants had achieved the best responsive (89.12%) and Survived (90.62%) with minimum dead (0.00%) and maximum contaminated (9.37%). The shoot tips showed the highest responsive, Survived and dead% (100, 87.40 and12.59% respectively) and minimum significant contaminated (0.00%). Also, the explants differed greatly in their percentages of responsive, survived, dead and contaminated at the different collected dates. The 6-benzylaminopurine (BAP) concentrations used (3 to 5.0 mg/L-1) demonstrated a similar behavior in terms of in vitro proliferation, with rate of 3.77 to 6.11, 4.33 to 8.88 and 3.33 to 7.44 shoots per explant for Okinawa, Nemared and Garnem peach rootstocks respectively, indicating that the number of shoots is genotype dependent. Hence, 5.0 mg/l BAP in combination with 0.2 mg/L-1 IBA is recommended for shoot proliferation of peach rootstocks since this combination treatment produced the higher significant values of average shoot proliferation % (96.29%) as well as number of shoot per explant (7.48) and average Leaves number/explant (16.33) than the other treatments.

show abstract

Approach to New peach rootstocks resistant to root-knot nematodes (Meloidogyne species) selected from local Mit-Ghamer peach cultivar

Cited by 8 publications

References 15 publications

Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocks a. In vitro Explant Surface Sterilization and Bud Proliferation

Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocks a. In vitro Explant Surface Sterilization and Bud Proliferation

In Vitro Explant Sterilization and Bud Proliferation Studies on Some Imported Peach Rootstocks

Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocksa. In vitro Explant Surface Sterilization and Bud Proliferation

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