Approach to improve the productivity of bioactive compounds of the cyanobacterium<i>Anabaena oryza</i>e using factorial design

Hamouda, Ragaa A.; Al-Saman, Mahmoud A.; El-Sabbagh, Sabha M.; El-Seoud, Ghada W. Abo; Hendawy, Abeer N.

doi:10.1016/j.ejbas.2017.06.003

Cited by 10 publications

(8 citation statements)

References 22 publications

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“…Algal extracts were prepared according to Hamouda et al (2017) . Different amounts of dried algal biomass (50, 100, 150 and 200 mg) were boiled in 10 mL distilled water for 30 min, cooled and centrifuged at 1,000 rpm for 5 min, after that the supernatants were taken as the algae extracts.…”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo hypolipidemic properties of the aqueous extract of Spirulina platensis, cultivated in colored flasks under artificial illumination

Al-Saman

Doleib

Ibrahim

et al. 2020

PeerJ

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Background Spirulina is blue-green algae that grows mainly in tropical and subtropical lakes and is commonly used due to its nutritional features including high concentrations of protein, vitamins, mineral salts, carotenoids and antioxidants. This study aimed to investigate the anti-hypercholesterolemic potential of aqueous extract of Spirulina platensis cultivated in different colored flasks under artificial illumination; in vitro and in the diet induced hypercholesterolemic Swiss albino mice. Methods Spirulina platensis was cultivated in red, blue, green and colorless Erlenmeyer flasks containing Zarrouk’s medium under aerobic conditions, with incessant illumination by artificial cool white fluorescent with light intensity of 2500 lux (35 µmol photon m−2 s−1). Chlorophyll a and total carotenoid contents were estimated using colorimetric methods, fatty acids composition was determined by GC-Mass, in vitro and in vivo anti-cholesterol assays were used in assessing the anti-hypercholesterolemia potential of obtained Spirulina cells. Results The results showed that the highest cell dry weight, chlorophyl a, and carotenoid of S. platensis were observed in colorless flasks and that the lowest values were recorded with the green colored flasks. Also, the hot water extract of S. platensis obtained from colorless flasks at a concentration of 15 mg/mL after 60 min of incubation exhibited the greatest reduction of cholesterol level. Gas chromatography-mass spectrometry analysis of S. platensis methanolic extract showed 15 bioactive compounds were identified and grouped according to their chemical structures. An experimental model of hypercholesterolemic mice had been examined for impact of S. platensis individually and combined with atorvastatin drug. All S. platensis groups resulted in a remarkable decrease in plasma total cholesterol, triglycerides and low density lipoprotein; and increase in high density lipoprotein. Conclusion The present study concluded that the hot aqueous extract of S. platensis developed in colorless flasks is recommended as a natural source for bioactive compounds, with anti-cholesterol and antioxidant potentialities.

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Section: Methodsmentioning

confidence: 99%

In vitro and in vivo hypolipidemic properties of the aqueous extract of Spirulina platensis, cultivated in colored flasks under artificial illumination

Al-Saman

Doleib

Ibrahim

et al. 2020

PeerJ

Self Cite

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“…The absorbance of the samples was measured at 515 nm. The free radical scavenging activity was calculated using the following equation: DPPH scavenging activity (%) (antioxidant activity, %) = ((A0-A1)/A0)*100 where: A0 is the absorbance of the control reaction and A1 is the absorbance of reaction mixture (Hamouda et al, 2017).…”

Section: Antioxidant Activity Of Ag-npsmentioning

confidence: 99%

Biosynthesis and Characterization of Silver Nanoparticles produced by Phormidium ambiguum and Desertifilum tharense

Hanna¹,

Hamouda²,

Goda³

et al. 2021

Preprint

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The world faces a challenge with pervasion of multidrug resistant bacteria which encouraged the scientists to develop and discover alternative ecofriendly and easy to produce new antibacterial agents. Two Egyptian cyanobacteria were isolated and identified according to 16S rRNA gene sequencing as Phormidium ambiguum and Desertifilum tharense . The sequences were deposited in the GenBank with accession numbers of MW762709 and MW762710 for Desertifilum tharense and Phormidium ambiguum, respectively. These isolates have the ability to produce silver nanoparticles (Ag-NPs) extra- and intracellularly under light and dark conditions. The results of UV-Vis analysis showed promising extracellular Ag-NPs synthesis by Desertifilum tharense and Phormidium ambiguum under light conditions. Therefore, these Ag-NPs were characterized and evaluated for antibacterial and antioxidant activity. TEM, SEM and XRD analyses revealed the spherical crystals with face-centered cubic structures and size range of 6.24–11.4 nm and 6.46–12.2 nm for Ag-NPs of Desertifilum tharense and Phormidium ambiguum , respectively. XRD and EDX results clearly confirmed the successful synthesis of Ag-NPs in its oxide form or chloride form. The FTIR spectrum data confirmed the presence of hydroxyl and amide groups. Desertifilum tharense Ag-NPs displayed the largest inhibition zone ranged from 9 mm against Micrococcus luteus ATCC 10240 to 25 mm against methicillin resistant S. aureus (MRSA) ATCC 43300. For Phormidium ambiguum Ag-NPs, the inhibition zone diameter was in a range of 9–18 mm. The Ag-NPs of Phormidium ambiguum exhibited the highest scavenging activity of 48.7% comparing with that of Desertifilum tharense which displayed 43.753%.

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“…The pure cultures of cyanobacteria were maintained in the culture room at 27 ± 2 • C. For regular experiments, cultures were grown in blue-green (BG-11) medium under 14-h:10-h light and dark photo period, the light intensity of 2400 Lux and at pH=7.0 (Hamouda et al, 2017;Mahfooz et al, 2017).…”

Section: Culture Conditions For Cyanobacteriamentioning

confidence: 99%

Media optimization for C-phycocyanin production in Plectonema sp. using response surface methodology and central composite design

Husain

Khan²,

Osama

et al. 2020

ijrps

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Cyanobacteria represent the richest sources of phycobiliproteins with especial reference to C-phycocyanin (C-PC), which in turn holds exhaustive therapeutic implications. Screening of several cyanobacterial strains namely Anabaena sp., Nostoc muscorum, Cylindrospermum sp., Plectonema sp., Scytonema sp., Spirulina sp., Synechococcous sp. and Tolypothrix sp. was carried out for their C-PC producing capacity, however the produced quantity of C-PC varies greatly among different strains. Owing to the crucial role of different media constituents on productivity of C-PC the current study was designed to optimize most appropriate media composition for augmented CPC production by selected superior producer. 36 factorial central composite design (CCD) dependent response surface methodology (RSM) was utilized to estimate the important medium components attributed with influencing C-PC productivity. RSM analysis of five independent coded factors including Na2CO3, K2HPO4, NaNO3, citric acid and EDTA were analyzed preceded by recognition of efficient variables for algal components production by Plectonema sp. Investigation of results revealed that the eminent medium components were NaCO3 (0.4 g/L); NaNO3 (0.5 g/L); K2HPO4 (2.8 g/L); citric acid (0.08 g/L) and EDTA (0.01 g/L) respectively. The optimized combination yielded 0.5536 mg/ml of C-PC. The increment of C-PC yield is R-Sq = 88.2%. Thus, our study led to the recognition of critical nutritional component that can be used further for enhanced productivity of C-PC.

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Approach to improve the productivity of bioactive compounds of the cyanobacteriumAnabaena oryzae using factorial design

Cited by 10 publications

References 22 publications

In vitro and in vivo hypolipidemic properties of the aqueous extract of Spirulina platensis, cultivated in colored flasks under artificial illumination

In vitro and in vivo hypolipidemic properties of the aqueous extract of Spirulina platensis, cultivated in colored flasks under artificial illumination

Biosynthesis and Characterization of Silver Nanoparticles produced by Phormidium ambiguum and Desertifilum tharense

Media optimization for C-phycocyanin production in Plectonema sp. using response surface methodology and central composite design

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