Approach to develop a standardized TLC-DPPH test for assessing free radical scavenging properties of selected phenolic compounds

Cieśla, Łukasz; Kryszeń, Jakub; Stochmal, Anna; Oleszek, Wiesław; Waksmundzka‐Hajnos, Monika

doi:10.1016/j.jpba.2012.06.007

Cited by 91 publications

(58 citation statements)

References 27 publications

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“…Gallic acid was used as standard for the analysis of total antioxidant capacity, and the DPPH radical scavenging activity was done following the methods described by Blios. 41 …”

Section: Qualitative and Quantitative Phytochemical Analysismentioning

confidence: 99%

Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

Arunachalam¹,

Arun²,

Annamalai³

et al. 2014

IJN

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Background Gymnema sylvestre is an ethno-pharmacologically important medicinal plant used in many polyherbal formulations for its potential health benefits. Silver nanoparticles (SNPs) were biofunctionalized using aqueous leaf extracts of G. sylvestre . The anticancer properties of the bioactive compounds and the biofunctionalized SNPs were compared using the HT29 human adenoma colon cancer cell line. Methods The preliminary phytochemical screening for bioactive compounds from aqueous extracts revealed the presence of alkaloids, triterpenes, flavonoids, steroids, and saponins. Biofunctionalized SNPs were synthesized using silver nitrate and characterized by ultraviolet–visible spectroscopy, scanning electron microscopy, energy-dispersive X-ray analysis, Fourier transform infrared spectroscopy, and X-ray diffraction for size and shape. The characterized biofunctionalized G. sylvestre were tested for its in vitro anticancer activity against HT29 human colon adenocarcinoma cells. Results The biofunctionlized G. sylvestre SNPs showed the surface plasmon resonance band at 430 nm. The scanning electron microscopy images showed the presence of spherical nanoparticles of various sizes, which were further determined using the Scherrer equation. In vitro cytotoxic activity of the biofunctionalized green-synthesized SNPs (GSNPs) indicated that the sensitivity of HT29 human colon adenocarcinoma cells for cytotoxic drugs is higher than that of Vero cell line for the same cytotoxic agents and also higher than the bioactive compound of the aqueous extract. Conclusion Our results show that the anticancer properties of the bioactive compounds of G. sylvestre can be enhanced through biofunctionalizing the SNPs using the bioactive compounds present in the plant extract without compromising their medicinal properties.

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“…Gallic acid was used as standard for the analysis of total antioxidant capacity, and the DPPH radical scavenging activity was done following the methods described by Blios. 41 …”

Section: Qualitative and Quantitative Phytochemical Analysismentioning

confidence: 99%

Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

Arunachalam¹,

Arun²,

Annamalai³

et al. 2014

IJN

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“…[16,21] The test was applied to fish out the active extracts. The samples giving positive results in this initial test were chosen for the subsequent analyses, as described below.…”

Section: Tlc-dpph • Testmentioning

confidence: 99%

“…[16,21] DPPH • solution was prepared by mixing 4 mg (10 À5 moles) DPPH • with 100 mL of methanol obtained 0.1 mM solution. Fresh reagent was always prepared prior to the analysis.…”

Section: Dpph • Radical-scavenging Assaymentioning

confidence: 99%

“…This convenience technique is simple to run and requires no specialized equipment. [16] The popularity of thin-layer chromatography combined with biological detection relays on a various of advantages of this technique, including: direct access to separated compounds, flexibility, possibility to assess the activity of individual compounds found in complex samples, high throughput. [17] Therefore TLC has been applied for the search of new antibacterial and antifungal drugs, as well as antioxidants and free radical scavengers.…”

Section: Introductionmentioning

confidence: 99%

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Radical Scavenging Activity of Instant Grits with Addition of Chamomile Flowers Determined by TLC–DPPH^• Test and by Spectrophotometric Method

Oniszczuk

Wojtunik

Oniszczuk

et al. 2015

Journal of Liquid Chromatography & Related Technologies

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Phenolic compounds possess multiple biological effects, including antioxidant activity. They may act as scavenger of free radicals or prevent their formation. The antioxidant properties of extruded grits enriched with 5%, 10%, and 20% addition of chamomile were evaluated. In order to determine antioxidant activity of samples, TLC-DPPH • test and spectrophotometric method, using DPPH (2,2 0 -diphenyl-1-picrylhydrazyl radical), were performed. The results of TLC-DPPH • test and spectrophotometric analysis indicated that antioxidant potential of corn grit increased with the addition of Matricaria chamomilla. The study findings confirmed that hightemperature extrusion process has no negative influence on antioxidant activity of polyphenolic compounds, which are present both in the raw and enriched with the Matricaria chamomilla final product.

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“…Recently, a number of DPPH• -based tests for the assessment of antioxidant activity have been developed. The evaluation of antioxidant capacity based on the amperometric reduction of DPPH• at the glassy carbon electrode was reported [14,15], TLC [16], HPLC techniques [17,18,19,20] and flow injection based methods for determination of scavenging capacity against DPPH• were also reported [21,22,23,24,25]. A DPPH• based optical sensor for screening of antioxidant activity was introduced by Steinberg et al [26].…”

Section: Introductionmentioning

confidence: 99%

New Approach for Measuring Antioxidant Activity Via a Graphite Sensor

El-Kosasy¹,

Hussien²,

Abdel‐Rahman³

2014

Port. Electrochim. Acta

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An ion selective membrane sensor from dioctyl phthalate as a plasticizer in a polymeric matrix of polyvinyl chloride (PVC) and β-cyclodextrin as an ionophore was constructed and evaluated according to IUPAC recommendations. Linear Nernstian response of DPPH• within the concentration ranges of 10 −6 to 10 −2 mol L −1 was obtained with average recovery 99.87±0.617. Nernstian slope of 58.5 mV/decade with excellent selectivity over the pH range of 3-8 was observed. The suggested method was standardized using butylated hydroxyl anisole (BHA). The 50% radical scavenging activity (IC 50 ) determined by the proposed sensor correlated well with that of the common spectrophotometric method based on scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH • ). An algorithm implemented in Microsoft Visual Basic ® 6.0 was used for calculating (IC 50 ) values which are 7.38 µg/mL ± 0.35, 89.98 µg/ mL ± 0.45 and 1.45 mg/ mL ± 1.50 for BHA, Paracetamol and Dipyridamole, respectively. The proposed sensor represents a simple and reproducible tool for measuring DPPH • scavenging activity of Paracetamol and Dipyridamole in bulk powder, pharmaceutical formulations and simulated intestinal fluid (SIF) without sophisticated separation techniques.

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Approach to develop a standardized TLC-DPPH test for assessing free radical scavenging properties of selected phenolic compounds

Cited by 91 publications

References 27 publications

Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

Radical Scavenging Activity of Instant Grits with Addition of Chamomile Flowers Determined by TLC–DPPH^• Test and by Spectrophotometric Method

New Approach for Measuring Antioxidant Activity Via a Graphite Sensor

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Approach to develop a standardized TLC-DPPH test for assessing free radical scavenging properties of selected phenolic compounds

Cited by 91 publications

References 27 publications

Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

Radical Scavenging Activity of Instant Grits with Addition of Chamomile Flowers Determined by TLC–DPPH• Test and by Spectrophotometric Method

New Approach for Measuring Antioxidant Activity Via a Graphite Sensor

Contact Info

Product

Resources

About

Radical Scavenging Activity of Instant Grits with Addition of Chamomile Flowers Determined by TLC–DPPH^• Test and by Spectrophotometric Method