Appraisal of Vitamin D3 Concentration in Dietary Supplement Marketed in Bangladesh using HPLC

Abstract

This research work provides information about the concentration level of fat soluble vitamin D3 in different types of dietary supplements marketed in Bangladesh. Selected twenty-five samples were taken for analysis because physicians are prescribing randomly and available in local market. Vitamin D3 concentration levels were quantified using HPLC with C18 column and diode array detector(DAD). The mobile phase was isocratic mode with ACN and methanol (60:40) and detection wavelength was (ƛ)265nm. Quantification level of D3 was ranging from 1. 80(µg.mlˉ1) to 24.91 (µg.mlˉ1) in analyzed samples. Quantification of vitamin D3 was conducted basis on the standard solution retention time (Rt) and peak area. The analyzed method provides excellent results with linearity, correlation co-efficient (r² ≥ 0.9995), LOD (0.0282, µg. mlˉ1), LOQ (0. 0941, µg. mlˉ1) and reproducibility with analyte recovery.