Appraisal of fluoroquinolone-loaded carubinose-linked hybrid nanoparticles for glycotargeting to alveolar macrophages

Maurya, Priyanka; Saklani, Ravi; Singh, Samipta; Nisha, Raquibun; Pal, Ravi Raj; Mishra, Nidhi; Singh, Priya; Kumar, Abhiram; Chourasia, Manish K.; Saraf, Shubhini A.

doi:10.1007/s13346-021-01055-9

Cited by 6 publications

(1 citation statement)

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“…For loading and entrapment efficiency calculation, the drug-loaded formulation was weighed and processed similarly as in specificity assay. The total loaded drug in the formulation was estimated and expressed as percentage fraction of the total formulation weight (loading efficiency) and weight of the total drug added to the formulation (entrapment efficiency) [ 23 , 24 ].…”

Section: Methodsmentioning

confidence: 99%

Validated HPLC-UV Method for Simultaneous Estimation of Paclitaxel and Doxorubicin Employing Ion Pair Chromatography: Application in Formulation Development and Pharmacokinetic Studies

Saklani

Tiwari

Yadav

et al. 2022

BioMed Research International

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Though paclitaxel (PTX) and doxorubicin (DOX) are amongst the most widely used and investigated drug pair for combination chemotherapy but surprisingly, not a single validated HPLC-UV method is available to analyze PTX and DOX simultaneously. So, herein a HPLC-UV method is developed and validated for the same, filling an indispensable gap in the literature. As these two moieties have characteristically different polarities, resolving them under the common chromatographic conditions is a challenging task. Herein, the principle of ion pair chromatography is utilized to resolve these two moieties on a C18 column employing an isocratic mobile phase comprised of acetonitrile and octane sulfonic acid buffer (67 : 37) and detected simultaneously at 231 nm using a UV detector only. The retention time is 4.4 and 7.2 min for PTX and DOX, respectively, with a total analysis time of less than 10 minutes, suitable for the formulation development and research, while LOQ is less than 0.066 μg/ml for both the drugs, suitable for the therapeutic drug monitoring at preclinical and clinical research setup. To substantiate the applicability of the developed method, a nanoformulation coloaded with PTX and DOX was designed and analyzed using the developed protocol. The method is also applied successfully to study the plasma kinetic profile of both the moieties simultaneously in Balb/c mice. Further, the method is validated as per the ICH guidelines fulfilling the unmet need of a validated analytical tool to simultaneously estimate PTX and DOX. Moreover, the results suggest that the principal of common ion chromatography demonstrated here can also be applied further for the simultaneous chromatographic separation of other polar and nonpolar moieties too. Consequently, the reported method surely will advance the toolset required for the precision-based combination chemotherapy.

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Section: Methodsmentioning

confidence: 99%