Applying biofluid 1H NMR-based metabonomic techniques to distinguish between HIV-1 positive/AIDS patients on antiretroviral treatment and HIV-1 negative individuals

Hewer, Raymond; Vorster, J.M.; Steffens, François E.; Meyer, Debra

doi:10.1016/j.jpba.2006.03.006

Cited by 64 publications

(55 citation statements)

References 14 publications

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“…In a preliminary study, Meyer and co-workers (Hewer et al 2006) have shown that metabolomics data contain sufficient information to distinguish HIV-positive/AIDS patients on ART from uninfected individuals.…”

Section: Introductionmentioning

confidence: 99%

“…Our initial study on HIV (Hewer et al 2006) is referred to in an overview by Nordström and Lewensohn (2010) as an example of a metabolomics investigation with potential to provide biomarkers for specific diseases. Here we present an extension of our NMR-based investigations, in which GC-MS was used to analyse the sera from HIV+ individuals.…”

Section: Introductionmentioning

confidence: 99%

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Qualitative serum organic acid profiles of HIV-infected individuals not on antiretroviral treatment

et al. 2011

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The first application of gas chromatography mass spectrometry (GC-MS) metabolomics to the analysis of organic acid profiles in sera of asymptomatic HIV-infected individuals (n = 18) compared to uninfected controls (n = 21), is reported here. Several organic acids are well-established diagnostic biomarkers of mitochondrial dysfunction, making the analysis of the organic acid metabolome well suited to monitoring the progressive disruption of mitochondrial structure and function during HIV infection. Using a multifaceted analyticalbioinformatics procedure, at least 10 of these metabolites could be linked to (1) disrupted mitochondrial metabolism, (2) changes in lipid metabolism and (3) oxidative stress, all of which are aberrations caused by HIV infection. Because of the role of the mitochondria in apoptosis, higher levels of this type of cell death in infected (compared to uninfected) individuals was used to support GC-MS data. This study demonstrates that mass spectrometry metabolomics detects biomarkers of mitochondrial dysfunction which could potentially be developed into indicators of HIV infection, perhaps also to monitor disease progression and the response to antiretroviral treatment (ART).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Qualitative serum organic acid profiles of HIV-infected individuals not on antiretroviral treatment

et al. 2011

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“…Similarly, 30 uninfected controls were also collected. Sample collection, preparation, and storage occurred as described by Hewer et al (2006) and Williams et al (2013). Table 1 presents the most relevant clinical information for the patients.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Previous studies from our group described the potential of both nuclear magnetic resonance (NMR) spectroscopy (Hewer et al, 2006;Philippeos et al, 2009) and mass spectrometry (MS) (Williams et al, 2012) in HIV/AIDS metabolomics. Here we explore the use of ATR-FTIR, a methodology incorporating a much simpler data collection approach, for the detection of metabolites in HIV-infected sera compared to negative controls with the secondary goal of determining whether metabolites detected in this manner would be comparable to those identified by NMR and MS-metabolomics and thus leading to the same conclusions.…”

Section: Introductionmentioning

confidence: 99%

Mid-ATR-FTIR Spectroscopic Profiling of HIV/AIDS Sera for Novel Systems Diagnostics in Global Health

Sitole

Steffens

Krüger

et al. 2014

OMICS: A Journal of Integrative Biology

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“…During HIV infection, host metabolism is influenced among other things by the virus [1][2][3], immune responses [4][5][6] and treatment [7,8]. HAART, primarily used to restore HIV-induced effects, prolongs the lifespan of infected individuals [8] and has subsequently resulted in HIV infection becoming a chronic condition, especially in developed countries.…”

Section: Introductionmentioning

confidence: 99%

UPLC-MS Metabonomics Reveals Perturbed Metabolites in HIV-Infected Sera

Williams¹,

Kgoadi²,

Steffens³

et al. 2014

CMB

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Immune responses to infection by the human immunodeficiency virus (HIV) and the use of highly active antiretroviral therapy (HAART) to treat HIV infection, contributes to metabolic irregularities in the host.Current methods for the extraction and identification of metabolites in biofluids generally make use of laborious, time-consuming protocols. Here, 96-well Ostro™ plates and filtration under positive pressure was used to facilitate the simultaneous, reproducible extraction of metabolites from multiple serum samples which were then analyzed by ultra-performance liquid chromatography mass spectrometry (UPLC-MS). The easy to use solid phase extraction (SPE) protocol eliminated numerous potential contaminants while the UPLC-MS detection of metabolites produced visibly different chromatograms for HIV negative (n=16), HIV+ (n=13) and HIV+HAART+ (n=15) serum samples. Linear discriminant analysis (LDA) amplified these differences, classified the groups with 100% accuracy and identified biomarkers explaining the greatest variances between the groups. The 21 metabolites altered by HIV and/or HAART primarily represented those linked to lipid and energy pathways which is where known metabolic changes associated with HIV infection occur. This work demonstrated for the first time that Ostro TM plates and UPLC-MS metabonomics was able to successfully identify distinct differences between the experimental groups and detected metabolites related to HAART and other drugs used in the treatment of HIV-associated conditions. The findings of this approach suggests a possible role for this methodology in disease prognosis as well as in the monitoring of treatment success or failure and linking treatment to metabolic complications.

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Applying biofluid 1H NMR-based metabonomic techniques to distinguish between HIV-1 positive/AIDS patients on antiretroviral treatment and HIV-1 negative individuals

Cited by 64 publications

References 14 publications

Qualitative serum organic acid profiles of HIV-infected individuals not on antiretroviral treatment

Qualitative serum organic acid profiles of HIV-infected individuals not on antiretroviral treatment

Mid-ATR-FTIR Spectroscopic Profiling of HIV/AIDS Sera for Novel Systems Diagnostics in Global Health

UPLC-MS Metabonomics Reveals Perturbed Metabolites in HIV-Infected Sera

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