This study investigated the acute changes in body composition that occur over the course of a competitive season in elite rugby league players. Twenty elite senior players from an English Super League rugby league team underwent a total-body dual-energy x-ray absorptiometry scan at three phases of a competitive season: preseason (February), mid-season (June) and post-season (September). Body mass, fat mass, lean mass, percentage body fat and bone mineral content were reported at each phase. Between the start and mid-point of the season, body mass, lean mass, fat mass and body fat percentage showed no significant change (p>0.05), however bone mineral content was significantly increased (+0.71%; 30.70 ± 38.00g; p<0.05). Between the mid-season and post-season phase, body mass and bone mineral content showed no significant change (p>0.05), however significant changes were observed in lean mass (-1.54%; 1.19 ± 1.43kg), fat mass (+4.09%; 0.57 ± 1.10kg) and body fat percentage (+4.98%; 0.78 ± 1.09%; p<0.05). The significant changes in body composition seen over the latter stages of the competitive season may have implications for performance capabilities at this important stage of competition. An increase in fat mass and decrease in lean mass may have a negative effect on the power/body mass ratio, and therefore may be a cause for concern for playing, coaching and medical staff.