Abstract:A partially purified lipase from the Pseudomonas aeruginosa strain (PSA-01) isolated from the palm oil fruit Elaeis guineensis was used as biocatalyst to produce fatty acid methyl esters (FAME). Lyophilized lipase supernatant (LLS) was used during the first step to screen the main variables (pH, temperature, stoichiometric oil:methanol ratio, water content and type of oil). Other variables which were identified during the screening assays (scale of reaction recipient, LLS amount and use of hexane to solubilize… Show more
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