Applications of urinary proteomics in biomarker discovery

Shao, Chen; Wang, Yan; Gao, Youhe

doi:10.1007/s11427-011-4162-1

Cited by 33 publications

(24 citation statements)

References 62 publications

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“…Urinalysis is known to be the first sign of kidney or urinary tract diseases and may offer important clues regarding the nature of pathological processes (29). The current study did not detect any adverse changes in urine routines when comparing the treatment and control groups.…”

Section: Discussioncontrasting

confidence: 46%

Evaluation of the acute and chronic toxicity of the jiangu capsules

et al. 2017

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Abstract. Saussureae involucratae Flos is the primary component of the jiangu capsule, which also consists of 12 types of natural medicine. It is a traditional Chinese medicine used to treat a number of conditions including arthralgia, cold and sore muscles, stiff limbs, inconvenient flexion and activity limitation caused by different types of osteoarthritis. The present study was performed to assess the safety of the jiangu capsule following acute and chronic administration in mice and rats. In the acute study, mice were treated with the jiangu capsule orally at 16 g/kg/day, the highest dose, for 14 days. In the chronic oral toxicity study, rats were treated with 2, 4 and 8 g/kg/day of the jiangu capsule for 6 months. The general condition, body weight changes, food and water consumption, hematological and biochemical parameters, urinalysis, systematic anatomy and organ weights of the rats were monitored and histopathological examination was performed at the end of 3 and 6 months' daily administration, and following 14 days of withdrawal. There were no signs of toxicity in the general condition and body weight of the mice was not significantly affected by the treatment. Furthermore, necropsy findings for the animals in the acute and chronic toxicity studies demonstrated that no mortalities had occurred. The results of the current study indicate that treatment with the jiangu capsule did not induce mortality or any detectable abnormalities in mice and rats.

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Section: Discussioncontrasting

confidence: 46%

Evaluation of the acute and chronic toxicity of the jiangu capsules

et al. 2017

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“…Urinary proteins are mainly composed of plasma proteins filtered by the glomerular barrier and proteins secreted from the kidney and urinary tract [14]. A previous study compared the kidney input (plasma) and output (urine) proteomes and divided urinary proteins into 3 categories, the plasma-only subproteome, the plasma-and-urine subproteome, and the urine-only subproteome [15].…”

Section: Resultsmentioning

confidence: 99%

Dynamic changes of urinary proteins in a focal segmental glomerulosclerosis rat model

Zhao

et al. 2014

Proteome Sci

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BackgroundIn contrast to blood, which has mechanisms to maintain a homeostatic internal environment, urine is more likely to reflect changes in the body. As urine accumulates all types of changes, identifying the precise cause of changes in the urine proteome is challenging and crucial in biomarker discovery. To reduce the effects of both genetic and environmental factors on the urinary proteome, this study used a rat model of adriamycin-induced nephropathy resembling human focal segmental glomerulosclerosis (FSGS) development.ResultsUrine samples were collected at before adriamycin administration and day3, 7, 11, 15 and 23 after. Urinary proteins were profiled by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Of 23 changed proteins with disease development, 20 have human orthologs, and 13 proteins were identified as stable in normal human urine, meaning that changes in these proteins are more likely to reflect disease. Fifteen of the identified proteins have not been established to function in FSGS development. Seven proteins were selected for verification in ten more rats as markers closely associated with disease severity by western blot.ConclusionWe identified proteins changed in different stages of FSGS in rat models, which may aid in biomarker development and the understanding of FSGS pathogenesis.

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“…Other than sampling serum or the brain, uric formaldehyde measurement has several advantages [28] , including the non-invasive nature of sample collection and the presence of fewer interfering proteins. Since proteins interfere with formaldehyde detection, urine was analyzed instead of serum which contains high protein levels.…”

Section: Introductionmentioning

confidence: 99%

Uric formaldehyde levels are negatively correlated with cognitive abilities in healthy older adults

Zhou

et al. 2014

Neurosci. Bull.

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Recent studies have shown that the abnormal accumulation of endogenous formaldehyde could be a critical factor in age-related cognitive decline. The aim of this study was to estimate the correlation between uric formaldehyde and general cognitive abilities in a community-based elderly population, and to measure the extent and direction in which the correlation varied with demographic characteristics. Using a double-blind design, formaldehyde in human urine was analyzed by high-performance liquid chromatography (n = 604), and general cognitive abilities were measured using the Montreal Cognitive Assessment (MoCA). Demographic characteristics, in terms of age, gender, residential region, and education were taken into consideration. We found that uric formaldehyde levels were inversely correlated with the MoCA score, and the concentration varied with demographic features: higher odds of a high formaldehyde level occurred among the less educated and those living in old urban or rural areas. In cytological experiments, the level of cellular formaldehyde released into the medium increased as SH-SY5Y and BV2 cells were incubated for three days. Formaldehyde in excess impaired the processes of N2a cells and neurites of primary cultured rat hippocampal cells. However, removal of formaldehyde markedly rescued and regenerated the processes of N2a cells. These results demonstrated a negative correlation between the endogenous formaldehyde and general cognitive abilities. High formaldehyde levels could be a risk factor for cognitive impairment in older adults, and could be developed as a non-invasive marker for detection and monitoring of age-related cognitive impairment.

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Applications of urinary proteomics in biomarker discovery

Cited by 33 publications

References 62 publications

Evaluation of the acute and chronic toxicity of the jiangu capsules

Evaluation of the acute and chronic toxicity of the jiangu capsules

Dynamic changes of urinary proteins in a focal segmental glomerulosclerosis rat model

Uric formaldehyde levels are negatively correlated with cognitive abilities in healthy older adults

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