Abstract:Fluorescent probes have found widespread use in biomedical sciences. Particularly since they can be targeted to cellular compartments and further more can report on the properties of their environment such as calcium concentration.Near infrared ultrafast lasers find increasing use for fluorescence applications since femtosecond pulses with a few milliwatts of average power are sufficient to induce significant two photon fluorescence from the probe when focussed into typical samples. The nonlinear optical excit… Show more
“…For quantitative measures of fluorescence intensity, researchers have typically normalized their measurements using rhodamine or fluorescein standards (5,33). This normalization of TPEF signals allows for comparisons between different laser power and detector gain settings.…”
Section: Intensity-based Measurements and The Optical Redox Ratiomentioning
Future work is needed to evaluate whether a combination of different biochemical and structural outcomes using these imaging techniques can provide complementary information regarding the utilization of specific metabolic pathways. Antioxid. Redox Signal. 00, 000-000.
“…For quantitative measures of fluorescence intensity, researchers have typically normalized their measurements using rhodamine or fluorescein standards (5,33). This normalization of TPEF signals allows for comparisons between different laser power and detector gain settings.…”
Section: Intensity-based Measurements and The Optical Redox Ratiomentioning
Future work is needed to evaluate whether a combination of different biochemical and structural outcomes using these imaging techniques can provide complementary information regarding the utilization of specific metabolic pathways. Antioxid. Redox Signal. 00, 000-000.
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