Applications of Thin-Layer Chromatography in Pharmaceutical Analyses

“…A detailed review about the biological role of selenium has recently been carried out by R. F. Burk, O. A. Levander, H. E. Ganther et al (1)(2)(3). In particular, some authors (4-7) pointed out the importance of measuring the levels of this element in whole blood and blood serum.…”

Section: Methodsmentioning

confidence: 99%

“…Of the many and varied methods described for their analysis, chromatography is one of the more specific. Paper and thin-layer chromatography have been used extensively (1), as have chromatography on polyamide (2), zinc ferrocyanide (3), and paper impregnated with strong cation-exchange resins (4). Recently, high pressure 1 Present address, Spectra-Physics Ltd., 17 Brick Knoll Park, St. Albans, Hertfordshire, U.K. anion exchange (5), reverse phase (6), and ion pair partition (7) methods have been published for specific sulfonamides.…”

mentioning

confidence: 99%

Determination of mefruside and its metabolites in urine by high performance liquid chromatography

Cj¹,

Ad²,

Da³

et al. 1977

Anal. Chem.

View full text Add to dashboard Cite

basis because of the possibility of variations in instrumental conditions as shown in an earlier study (21). For this purpose, multiple ion detection using an accelerating voltage alternator (AVA) (23) and simultaneous monitoring of several ions, including one or more of the isotopically labeled internal standard, is necessary. Ideally, the internal standard should be subjected to the identical sample preparation and derivatization conditions as the compound in question. This was not possible in this specific case, as the labeled analogue of RT3 had to be prepared separately during esterification. This problem can be alleviated by using a compound containing deuterium atoms on the thyronine phenyl rings, which, of course, would be a major project in itself. Nevertheless, the methodology developed for the analysis of free RTB by Dialysis-GC-MS-SIM can be applied to the analysis of all the thyroid hormones and their precursors as their HFB-ME derivatives.

show abstract

Section: Literature Citedmentioning

confidence: 99%