Applications of the second virial coefficient: protein crystallization and solubility

Wilson, William W.; DeLucas, Lawrence J.

doi:10.1107/s2053230x1400867x

Cited by 31 publications

(24 citation statements)

References 75 publications

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“…Candidate vaccines have been tested as monovalent (single virus), bivalent (two viruses), trivalent (three viruses) and tetravalent (all four serotype viruses) vaccines in Thai volunteers. They were found to be safe and immunogenic in both adults and children [ 56 ] . Four serotypes of monovalent live attenuated dengue virus vaccine candidates were tested for immunogenicity, which were then combined into a tetravalent formulation and given to ten volunteers.…”

Section: Discussionmentioning

confidence: 99%

In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever

Vijayakumar¹,

Venkatachalam²,

Prabhu³

et al. 2018

J Biomed Res

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Reverse vaccinology method was used to predict the monovalent peptide vaccine candidate to produce antibodies for therapeutic purpose and to predict tetravalent vaccine candidate to act as a common vaccine to cover all the dengue virus serotypes. Envelope (E)-proteins of DENV-1-4 serotypes were used for vaccine prediction using NCBI, Uniprot/Swissprot, Swiss-prot viewer, VaxiJen V2.0, TMHMM, BCPREDS, Propred-1, Propred and MHC Pred. E-proteins of DENV-1-4 serotypes were identified as antigen from which T cell epitopes, through B cell epitopes, were predicted to act as peptide vaccine candidates. Each selected T cell epitope of E-protein was confirmed to act as vaccine and to induce complementary antibody against particular serotype of dengue virus. Chimeric tetravalent vaccine was formed by the conjugation of four vaccines, each from four dengue serotypes to act as a common vaccine candidate for all the four dengue serotypes. It can be justifiably concluded that the monovalent 9-mer T cell epitope for each DENV serotype can be used to produce specific antibody against dengue virus and a chimeric common tetravalent vaccine candidate to yield a comparative vaccine to cover any of the four dengue virus serotype. This vaccine is expected to be highly immunogenic against dengue fever.

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Section: Discussionmentioning

confidence: 99%

In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever

Vijayakumar¹,

Venkatachalam²,

Prabhu³

et al. 2018

J Biomed Res

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show abstract

“…However, it is nonetheless true that an improved understanding of mechanism will likely propel future advances. An example of a potentially enabling avenue of research involves the osmotic second virial coefficient, a dilute solution parameter that has been demonstrated to predict crystallization behavior (a recent review in this series was devoted to this topic; Wilson & Delucas, 2014). Because the second virial coefficient reflects pairwise interactions between protein molecules (or between protein-detergent complexes), it provides a window into the molecular interactions driving assembly of the crystal lattice.…”

Section: Mechanistic Approaches To Crystallization Of Proteindetergenmentioning

confidence: 99%

Membrane proteins, detergents and crystals: what is the state of the art?

Loll

2014

Acta Crystallogr F Struct Biol Commun

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At the time when the first membrane-protein crystal structure was determined, crystallization of these molecules was widely perceived as extremely arduous. Today, that perception has changed drastically, and the process is regarded as routine (or nearly so). On the occasion of the International Year of Crystallography 2014, this review presents a snapshot of the current state of the art, with an emphasis on the role of detergents in this process. A survey of membrane-protein crystal structures published since 2012 reveals that the direct crystallization of protein-detergent complexes remains the dominant methodology; in addition, lipidic mesophases have proven immensely useful, particularly in specific niches, and bicelles, while perhaps undervalued, have provided important contributions as well. Evolving trends include the addition of lipids to protein-detergent complexes and the gradual incorporation of new detergents into the standard repertoire. Stability has emerged as a critical parameter controlling how a membrane protein behaves in the presence of detergent, and efforts to enhance stability are discussed. Finally, although discovery-based screening approaches continue to dwarf mechanistic efforts to unravel crystallization, recent technical advances offer hope that future experiments might incorporate the rational manipulation of crystallization behaviors.

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“…Taken their size distribution. It is also an effective technique to probe for potential crystallization conditions and solubility, as reviewed by Wilson & DeLucas (2014).…”

Section: Other Techniquesmentioning

confidence: 99%

Identifying, studying and making good use of macromolecular crystals

et al. 2014

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Structural biology has contributed tremendous knowledge to the understanding of life on the molecular scale. The Protein Data Bank, a depository of this structural knowledge, currently contains over 100 000 protein structures, with the majority stemming from X-ray crystallography. As the name might suggest, crystallography requires crystals. As detectors become more sensitive and X-ray sources more intense, the notion of a crystal is gradually changing from one large enough to embellish expensive jewellery to objects that have external dimensions of the order of the wavelength of visible light. Identifying these crystals is a prerequisite to their study. This paper discusses developments in identifying these crystals during crystallization screening and distinguishing them from other potential outcomes. The practical aspects of ensuring that once a crystal is identified it can then be positioned in the X-ray beam for data collection are also addressed.

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Applications of the second virial coefficient: protein crystallization and solubility

Cited by 31 publications

References 75 publications

In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever

In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever

Membrane proteins, detergents and crystals: what is the state of the art?

Identifying, studying and making good use of macromolecular crystals

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