Applications of the phage display technology in molecular biology, biotechnology and medicine

Pierzynowska, Karolina; Morcinek-Orłowska, Joanna; Gaffke, Lidia; Jaroszewicz, Weronika; Skowron, Piotr; Węgrzyn, Grzegorz

doi:10.1080/1040841x.2023.2219741

Cited by 12 publications

(6 citation statements)

References 270 publications

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“…Phage display was invented in 1981 ( 108 ). It is a type of genetic modification technique, in which genetic modification of phages enables the target gene to bind to the phage shell protein, so that the corresponding fragment can be expressed on the phage surface when the phage is infected ( 109 ). Researchers found that phages have several advantages.…”

Section: Phage Displaymentioning

confidence: 99%

Recent advances in the exploration and discovery of SARS-CoV-2 inhibitory peptides from edible animal proteins

Kong,

Wang,

Zhong

et al. 2024

Front. Nutr.

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The severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), which causes the coronavirus disease 2019 (COVID-19), is spreading worldwide. Although the COVID-19 epidemic has passed its peak of transmission, the harm it has caused deserves our attention. Scientists are striving to develop medications that can effectively treat COVID-19 symptoms without causing any adverse reactions. SARS-CoV-2 inhibitory peptides derived from animal proteins have a wide range of functional activities in addition to safety. Identifying animal protein sources is crucial to obtaining SARS-CoV-2 inhibitory peptides from animal sources. This review aims to reveal the mechanisms of action of these peptides on SARS-CoV-2 and the possibility of animal proteins as a material source of SARS-CoV-2 inhibitory peptides. Also, it introduces the utilization of computer-aided design methods, phage display, and drug delivery strategies in the research on SARS-CoV-2 inhibitor peptides from animal proteins. In order to identify new antiviral peptides and boost their efficiency, we recommend investigating the interaction between SARS-CoV-2 inhibitory peptides from animal protein sources and non-structural proteins (Nsps) using a variety of technologies, including computer-aided drug approaches, phage display techniques, and drug delivery techniques. This article provides useful information for the development of novel anti-COVID-19 drugs.

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Section: Phage Displaymentioning

confidence: 99%

Recent advances in the exploration and discovery of SARS-CoV-2 inhibitory peptides from edible animal proteins

Kong,

Wang,

Zhong

et al. 2024

Front. Nutr.

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“…Nowadays, phage display technology is commonly used for drug development because it allows for an easy and effective screening of large pools of peptides or antibody variable domains. The development of the method paved the way for a wider use of bacteriophages in nanotechnology and biomedical studies [ 208 ]. The phage display technique is a promising tool that may be useful in the treatment of glioma.…”

Section: Potential Of Phage Application In Targeted Therapy For Brain...mentioning

confidence: 99%

Phage Interactions with the Nervous System in Health and Disease

Jędrusiak,

Fortuna,

Majewska

et al. 2023

Cells

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The central nervous system manages all of our activities (e.g., direct thinking and decision-making processes). It receives information from the environment and responds to environmental stimuli. Bacterial viruses (bacteriophages, phages) are the most numerous structures occurring in the biosphere and are also found in the human organism. Therefore, understanding how phages may influence this system is of great importance and is the purpose of this review. We have focused on the effect of natural bacteriophages in the central nervous system, linking them to those present in the gut microbiota, creating the gut-brain axis network, as well as their interdependence. Importantly, based on the current knowledge in the field of phage application (e.g., intranasal) in the treatment of bacterial diseases associated with the brain and nervous system, bacteriophages may have significant therapeutic potential. Moreover, it was indicated that bacteriophages may influence cognitive processing. In addition, phages (via phage display technology) appear promising as a targeted therapeutic tool in the treatment of, among other things, brain cancers. The information collected and reviewed in this work indicates that phages and their impact on the nervous system is a fascinating and, so far, underexplored field. Therefore, the aim of this review is not only to summarize currently available information on the association of phages with the nervous system, but also to stimulate future studies that could pave the way for novel therapeutic approaches potentially useful in treating bacterial and non-bacterial neural diseases.

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“…Furthermore, phage display technology, allowing us to expose virtually any peptides on the surface of phage virions when fused to one of coat proteins [25], has revolutionized the biotechnological search for compounds able to bind to any specific target molecules. This technology is useful in a wide spectrum of applications, such as improving medical diagnostics, searching for novel drugs, developing effective vaccines, inactivating toxins, producing novel materials (including nanomaterials), and others [26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Bacteriophages—Dangerous Viruses Acting Incognito or Underestimated Saviors in the Fight against Bacteria?

Podlacha,

Węgrzyn,

Węgrzyn

2024

IJMS

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The steadily increasing number of drug-resistant bacterial species has prompted the search for alternative treatments, resulting in a growing interest in bacteriophages. Although they are viruses infecting bacterial cells, bacteriophages are an extremely important part of the human microbiota. By interacting with eukaryotic cells, they are able to modulate the functioning of many systems, including the immune and nervous systems, affecting not only the homeostasis of the organism, but potentially also the regulation of pathological processes. Therefore, the aim of this review is to answer the questions of (i) how animal/human immune systems respond to bacteriophages under physiological conditions and under conditions of reduced immunity, especially during bacterial infection; (ii) whether bacteriophages can induce negative changes in brain functioning after crossing the blood–brain barrier, which could result in various disorders or in an increase in the risk of neurodegenerative diseases; and (iii) how bacteriophages can modify gut microbiota. The crucial dilemma is whether administration of bacteriophages is always beneficial or rather if it may involve any risks.

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Applications of the phage display technology in molecular biology, biotechnology and medicine

Cited by 12 publications

References 270 publications

Recent advances in the exploration and discovery of SARS-CoV-2 inhibitory peptides from edible animal proteins

Recent advances in the exploration and discovery of SARS-CoV-2 inhibitory peptides from edible animal proteins

Phage Interactions with the Nervous System in Health and Disease

Bacteriophages—Dangerous Viruses Acting Incognito or Underestimated Saviors in the Fight against Bacteria?

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