Applications of the green fluorescent protein as a molecular marker in environmental microorganisms

Errampalli, Deena; Leung, Ka Yin; Cassidy, Mike; Kostrzynska, Magdalena; Blears, M.; Lee, H.; Trevors, J. T.

doi:10.1016/s0167-7012(99)00024-x

Cited by 187 publications

(115 citation statements)

References 47 publications

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“…This work presents a quantitative phenotypic characterization of nongrowing cells (29), which constitute the most common lifestyle in the wild and should find relevance in different ecological environments where growth arrest plays a major role, for example in biofilms (30,31). An approach similar to the one presented in this study might shed light on the processes leading to the dormancy of subpopulations in different systems, such as in subpopulations of viable but nonculturable bacteria (32) or those of quiescent cancer cells (33).…”

Section: Measurement Of the Induction Dynamics Of Persisters After Thmentioning

confidence: 99%

Single-cell protein induction dynamics reveals a period of vulnerability to antibiotics in persister bacteria

Gefen

Gabay

Mumcuoglu

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

144

153

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Phenotypic variability in populations of cells has been linked to evolutionary robustness to stressful conditions. A remarkable example of the importance of cell-to-cell variability is found in bacterial persistence, where subpopulations of dormant bacteria, termed persisters, were shown to be responsible for the persistence of the population to antibiotic treatments. Here, we use microfluidic devices to monitor the induction of fluorescent proteins under synthetic promoters and characterize the dormant state of single persister bacteria. Surprisingly, we observe that protein production does take place in supposedly dormant bacteria, over a narrow time window after the exit from stationary phase. Only thereafter does protein production stop, suggesting that differentiation into persisters fully develops over this time window and not during starvation, as previously believed. In effect, we observe that exposure of bacteria to antibiotics during this time window significantly reduces persistence.

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Section: Measurement Of the Induction Dynamics Of Persisters After Thmentioning

confidence: 99%

Single-cell protein induction dynamics reveals a period of vulnerability to antibiotics in persister bacteria

Gefen

Gabay

Mumcuoglu

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

144

153

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“…Reporter-genes based bacterial constructs appear as very useful tools for the analysis of promoter activity in a variety of Gram-negative population of bacteria and at single-cell levels [11,14]. Silva-Rocha and de Lorenzo 2012 [38] described a bicistronic reporter system integrated to the bacterial chromosome, which utilizes an artifi cial operon containing gfp and lacZ genes.…”

Section: Reporter-genes Based Bacteria For Environmental Applicationsmentioning

confidence: 99%

Environmental Application of Reporter-Genes Based Biosensors for Chemical Contamination Screening

Matejczyk¹,

Rosochacki²

2014

Archives of Environmental Protection

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Abstract:The paper presents results of research concerning possibilities of applications of reporter-genes based microorganisms, including the selective presentation of defects and advantages of different new scientifi c achievements of methodical solutions in genetic system constructions of biosensing elements for environmental research. The most robust and popular genetic fusion and new trends in reporter genes technology -such as LacZ (β-galactosidase), xylE (catechol 2,3-dioxygenase), gfp (green fl uorescent proteins) and its mutated forms, lux (prokaryotic luciferase), luc (eukaryotic luciferase), phoA (alkaline phosphatase), gusA and gurA (β-glucuronidase), antibiotics and heavy metals resistance are described. Reporter-genes based biosensors with use of genetically modifi ed bacteria and yeast successfully work for genotoxicity, bioavailability and oxidative stress assessment for detection and monitoring of toxic compounds in drinking water and different environmental samples, surface water, soil, sediments.

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“…The application of fluorescence biosensors in environmental protection applications [20][21][22][23][24][25], medical diagnostics [26][27][28][29][30][31][32][33][34][35], and industries [36][37][38][39][40][41][42][43][44][45] also is growing.…”

mentioning

confidence: 99%

Enzyme-Based Fluorescent Biosensors and Their Environmental, Clinical and Industrial Applications

Kłos-Witkowska¹

2015

Pol. J. Environ. Stud.

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The last two decades has seen a significant increase in interest in biosensors. According to the Web of Science database, the number of scientific publications over the past 20 years increased from 309 in 1993 up to 3,467 in 2013 (Fig. 1).The demand for developing new detection methods, increasing interest in visualization techniques [1][2][3], emphasis placed on new drug discovery programs [4], diagnostic test development [5][6][7], and attempts to explain cellular action mechanisms and to trace a cell's metabolism pathway [7,8], and the possibility of using biosensors in numerous fields of application, (for example: medicine [9-11], environmental protection [12-14, 16-17], food industry [18], and defense industry [19]) has results in an increasing number of research projects targeted at biosensor design and fabrication. Biosensors used in environmental monitoring measure the toxicity effect based on the detection of a chemical compound or compound group by selective recognition of a biomolecule in the receptor layer and then by detection of a signal after passing through the transducer layer [16].The application of fluorescence biosensors in environmental protection applications [20][21][22][23][24][25], medical diagnostics [26][27][28][29][30][31][32][33][34][35], and industries [36][37][38][39][40][41][42][43][44][45] also is growing.Pol. J. Environ. Stud. Vol. 24, No. 1 (2015) AbstractEnzyme-based fluorescence biosensors and their applications in environmental protection, medicine, and industry are described. Biosensors used in environmental protection measure toxicity effects. A chemical compound or group of compounds is detected by the recognition of molecules in the receptor layer and then by detecting a signal passing through the transducer layer. Biosensors are classified according to the transduction method. Special emphasis is placed on optical biosensors, especially fluorescent biosensors, and such measurement techniques as FRET (Fröster resonance energy transfer), FLIM (fluorescence lifetime imaging), FCS (fluorescence correlation spectroscopy), and changes in fluorescence intensity. The phenomenon of fluorescence in biosensors and the selection of appropriate methods are described. The use of enzymes in the receptor layer and enzyme classification according to its category and functions used for analyte detection are presented. The fluorescence properties of enzymes resulting from possessing such cofactors as flavin or heme (prosthetic) groups are discussed. Several methods for enzyme immobilization, namely entrapment, adsorption, covalent immobilization, cross linking, and affinity interaction are described, and the use of enzymatic fluorescence biosensors in the detection of analytes is presented.

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Applications of the green fluorescent protein as a molecular marker in environmental microorganisms

Cited by 187 publications

References 47 publications

Single-cell protein induction dynamics reveals a period of vulnerability to antibiotics in persister bacteria

Single-cell protein induction dynamics reveals a period of vulnerability to antibiotics in persister bacteria

Environmental Application of Reporter-Genes Based Biosensors for Chemical Contamination Screening

Enzyme-Based Fluorescent Biosensors and Their Environmental, Clinical and Industrial Applications

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