Applications of surface-enhanced Raman spectroscopy (SERS) for biosensing: an analysis of reproducible, commercially available substrates

Alexander, Troy A.

doi:10.1117/12.630659

Cited by 20 publications

(12 citation statements)

References 2 publications

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“…[48][49][50][51][52] These substrates were developed using Si-based semiconductor fabrication techniques. 52 Klarite substrates are fabricated using a well-defined Silicon fabrication technique in which a silicon diode mask is defined by optical lithography, and then KOH surface etched.…”

Section: Klarite Sers Substratesmentioning

confidence: 99%

The development of Army relevant peptide-based surface enhanced Raman scattering (SERS) sensors for biological threat detection

et al. 2016

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The utility of peptide-based molecular sensing for the development of novel biosensors has resulted in a significant increase in their development and usage for sensing targets like chemical, biological, energetic and toxic materials. Using peptides as a molecular recognition element is particularly advantageous because there are several mature peptide synthesis protocols that already exist, peptide structures can be tailored, selected and manipulated to be highly discerning towards desired targets, peptides can be modified to be very stable in a host of environments and stable under many different conditions, and through the development of bifunctionalized peptides can be synthesized to also bind onto desired sensing platforms (various metal materials, glass, etc.). Two examples of the several Army relevant biological targets for peptide-based sensing platforms include Ricin and Abrin. Ricin and Abrin are alarming threats because both can be weaponized and there is no antidote for exposure. Combining the sensitivity of SERS with the selectivity of a bifunctional peptide allows for the emergence of dynamic hazard sensor for Army application.

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Section: Klarite Sers Substratesmentioning

confidence: 99%

The development of Army relevant peptide-based surface enhanced Raman scattering (SERS) sensors for biological threat detection

et al. 2016

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“…Following the fabrication of the underlying silicon surface, approximately 100 nm of gold (Au) is deposited onto the surface. It is designed for one time use and packed in a vacuum packaging to avoid degradation [34].…”

Section: A Substratesmentioning

confidence: 99%

Surface-Enhanced Raman spectral analysis of substrates for salivary based disease detection

Radzol

Lee

Mansor

et al. 2012

2012 IEEE 8th International Colloquium on Signal Processing and Its Applications

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Raman Spectroscopy is a mean to study the molecular structure property of solid, liquid and gases from its scattering spectrum. It offers a detailed biochemical fingerprint for identifying the unknown molecule. Since the amount of inelastic scattering is infinitesimally small relative to that of the elastic scattering, the Raman signal emitted is extremely weak, rendering limited applications. Surface Enhanced Raman Spectroscopy augments the detection sensitivity of Raman spectroscopy with nano-sensing chip as substrate, enabling amplification of Raman signal by a factor of 10 4 to 10 9 . This innovation is made possible with the integration of the latest in optical sensing technology and nanotechnology. With this improvement, SERS has shown its niche in tracing molecular structure, especially in marking abnormal biological molecules such as cancer, conjunctivitis, AIDS. The quality of spectrum is highly dependent on the substrate type and laser wavelength. Our work here examines the spectral characteristics of 3 candidate substrate types and wavelength for application in salivary based disease detection. The spectrum intensity enhancement, oxidation and fluorescence effects are also investigated. This study finds that adoption of gold or Klarite® as substrate and 785nm as wavelength are appropriate for salivary based disease detection using SERS technique. The latter will be preferred if cost effectiveness is important.

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“…(80,(95)(96)(97)(98) These substrates were developed using Si-based semiconductor fabrication techniques. (98) Klarite substrates are fabricated using a well defined silicon fabrication technique in which a silicon diode mask is defined by optical lithography, and then potassium hydroxide (KOH) surface etched.…”

Section: Introductionmentioning

confidence: 99%

Army relevant Biological Hazards Detection with Commercial SERS substrates

Farrell

Pellegrino

2012

SPIE Proceedings

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There is an increasing need and challenge for early rapid and accurate detection, identification, and quantification of chemical, biological, and energetic hazards in many fields of interest (e.g., medical, environmental, industrial, and defense applications). Increasingly to meet these challenges, researchers are turning to interdisciplinary approaches combining spectroscopy with nanoscale platforms to create technologies that offer viable and novel solutions for today's sensing needs. One technology that has gained increasing popularity to meet these needs is surface enhanced Raman scattering (SERS). SERS is particularly advantageous as it does not suffer from interferences from water, requires little to no sample preparation, is robust and can be used in numerous environments, is relatively insensitive to the wavelength of excitation employed and produces a narrow-band spectral signature unique to the molecular vibrations of the analyte. SERS enhancements (chemical and electromagnetic) are typically observed on metalized nanoscale roughened surfaces. For ideal SERS sensing, a commercially available uniform and reproducible nanoscale surface demonstrating high sensitivity are desirable. Additionally, if these surfaces can be modified for the selective sensing of hazard materials, an ideal sensor platform for dynamic in field measurements can be imagined. In this proceedings paper, preliminary efforts towards the characterization and application of commercially available next generation Klarite substrates will be demonstrated. Additionally, efforts toward chemical modification of these substrates, through peptide recognition elements, can be used for the targeted sensing of hazardous materials.

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Applications of surface-enhanced Raman spectroscopy (SERS) for biosensing: an analysis of reproducible, commercially available substrates

Cited by 20 publications

References 2 publications

The development of Army relevant peptide-based surface enhanced Raman scattering (SERS) sensors for biological threat detection

The development of Army relevant peptide-based surface enhanced Raman scattering (SERS) sensors for biological threat detection

Surface-Enhanced Raman spectral analysis of substrates for salivary based disease detection

Army relevant Biological Hazards Detection with Commercial SERS substrates

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