Applications of single‐cell multi‐omics sequencing in deep understanding of brain diseases

Zhang, Xu; Lü, Ping; Shen, Xianfeng

doi:10.1002/ctd2.95

Cited by 2 publications

(2 citation statements)

References 493 publications

(731 reference statements)

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“…Current single-cell ecDNA detection methods can be broadly categorized into two primary groups. The first group involves the use of computational tools and WGS data obtained from NGS 56 or TGS 57, 58 to identify circularization sites. However, distinguishing between ecDNA and HSR using this approach can be challenging, because they have the same breakpoint sequences.…”

Section: Discussionmentioning

confidence: 99%

Droplet Hi-C for Fast and Scalable Profiling of Chromatin Architecture in Single Cells

Chang,

Xie,

Taylor

et al. 2024

Preprint

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Comprehensive analysis of chromatin architecture is crucial for understanding the gene regulatory programs during development and in disease pathogenesis, yet current methods often inadequately address the unique challenges presented by analysis of heterogeneous tissue samples. Here, we introduce Droplet Hi-C, which employs a commercial microfluidic device for high-throughput, single-cell chromatin conformation profiling in droplets. Using Droplet Hi-C, we mapped the chromatin architecture at single-cell resolution from the mouse cortex and analyzed gene regulatory programs in major cortical cell types. Additionally, we used this technique to detect copy number variation (CNV), structural variations (SVs) and extrachromosomal DNA (ecDNA) in cancer cells, revealing clonal dynamics and other oncogenic events during treatment. We further refined this technique to allow for joint profiling of chromatin architecture and transcriptome in single cells, facilitating a more comprehensive exploration of the links between chromatin architecture and gene expression in both normal tissues and tumors. Thus, Droplet Hi-C not only addresses critical gaps in chromatin analysis of heterogeneous tissues but also emerges as a versatile tool enhancing our understanding of gene regulation in health and disease.

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Section: Discussionmentioning

confidence: 99%

Droplet Hi-C for Fast and Scalable Profiling of Chromatin Architecture in Single Cells

Chang,

Xie,

Taylor

et al. 2024

Preprint

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“…SCS has emerged as a critical tool for mapping the cellular landscape of the brain, revealing new cell types and pathways involved in neurological diseases. For instance, in Alzheimer's disease research, SCS has identified specific subpopulations of neurons and glial cells that are disproportionately affected by pathological processes, offering new targets for therapeutic intervention 4,5 . Similarly, in the study of psychiatric disorders, SCS is uncovering the cellular basis of disease phenotypes, paving the way for novel diagnostic markers and treatment strategies.…”

Section: Figurementioning

confidence: 99%

Advancing precision medicine through single‐cell sequencing: Insights and implications

Zhang,

Gao,

Yang

et al. 2024

Clinical and Translational Dis

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BackgroundSingle‐cell sequencing (SCS) marks the advent of a transformative period in biomedical studies, enabling unprecedented insight into the cellular intricacies of health and disease.MethodsBy dissecting the genetic, epigenetic and proteomic landscapes at the single‐cell level, SCS transcends traditional bulk sequencing methodologies, illuminating the heterogeneity and dynamics of individual cells.ResultsThis analytical leap facilitates a deeper understanding of disease mechanisms, offers novel diagnostic and therapeutic targets and underpins the development of precision medicine across diverse fields such as neurology, oncology and immunology.ConclusionsDespite its profound potential, SCS encounters challenges, including complex sample preparation, sophisticated data analysis and cost considerations. Nevertheless, ongoing advancements promise to overcome these barriers, integrating SCS with other omics data and leveraging machine learning to enhance biological understanding and clinical application. With the advancement of SCS technologies, personalised healthcare might be fundamentally altered, facilitating tailored and efficacious treatment strategies.

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Applications of single‐cell multi‐omics sequencing in deep understanding of brain diseases

Cited by 2 publications

References 493 publications

Droplet Hi-C for Fast and Scalable Profiling of Chromatin Architecture in Single Cells

Droplet Hi-C for Fast and Scalable Profiling of Chromatin Architecture in Single Cells

Advancing precision medicine through single‐cell sequencing: Insights and implications

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