Applications of serum albumins in delivery systems: Differences in interfacial behaviour and interacting abilities with polysaccharides

Aguilera-Garrido, Aixa; Castillo-Santaella, Teresa del; Yang, Yan; Galisteo‐González, F.; Gálvez-Ruiz, Marı́a José; Molina-Bolı́var, J.A.; Holgado-Terriza, Juan A.; Cabrerizo-Vílchez, Miguel A.; Maldonado-Valderrama, Julia

doi:10.1016/j.cis.2021.102365

Cited by 48 publications

(21 citation statements)

References 148 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it can be inferred that both macrocycles are located near the Trp residue in BSA molecule [ 42 ]. It is known that the BSA molecule contains two different tryptophan residues Trp134 and Trp212, which differ significantly in their environment [ 45 ]. The interactions between BSA with 3 and 4 are additionally studied via fluorescence spectroscopy at the excitation wavelength is 295 nm.…”

Section: Resultsmentioning

confidence: 99%

Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin

Yakimova

Kunafina

Mostovaya

et al. 2022

IJMS

View full text Add to dashboard Cite

The therapeutic application of serum albumin is determined by the relative content of the monomeric form compared to dimers, tetramers, hexamers, etc. In this paper, we propose and develop an approach to synthesize the cone stereoisomer of p-tert-butylthiacalix[4]arene with sulfobetaine fragments stabilization of monomeric bovine serum albumin and preventing aggregation. Spectral methods (UV-vis, CD, fluorescent spectroscopy, and dynamic light scattering) established the influence of the synthesized compounds on the content of monomeric and aggregated forms of BSA even without the formation of stable thiacalixarene/protein associates. The effect of thiacalixarenes on the efficiency of protein binding with the antibiotic ciprofloxacin was shown by fluorescence spectroscopy. The binding constant increases in the presence of the macrocycles, likely due to the stabilization of monomeric forms of BSA. Our study clearly shows the potential of this macrocycle design as a platform for the development of the fundamentally new approaches for preventing aggregation.

show abstract

Section: Resultsmentioning

confidence: 99%

Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin

Yakimova

Kunafina

Mostovaya

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…It is known that ICG is bound to the hydrophobic core of human serum albumin, which results in some chemical stabilization of ICG [27]. BSA can act as a transporter in the body, delivering a variety of exogenous or endogenous substances, making it one of the most valued serum albumins in the biomedical field because of its high water solubility and good biocompatibility [28,29]. Thus, modification of NIR fluorescent dye ICG with BSA can increase its solubility and biocompatibility in the present study.…”

Section: Discussionmentioning

confidence: 99%

Indocyanine green for targeted imaging of the gall bladder and fluorescence navigation

Wang

Yang

Mei

et al. 2022

Journal of Biophotonics

View full text Add to dashboard Cite

Researchers nowadays have devoted extra attention to the different biomedical applications of indocyanine green (ICG), a US Food and Drug Administration‐approved fluorescent compound in the fields such as drug delivery, medical imaging and disease diagnosis. In addition, hepatic function evaluation could be conducted by using ICG before surgical procedures and angiographic assessment of blood. Therefore, ICG will be expected to be excellent imaging and targeting agent in various preclinical and clinical model systems. However, whether ICG possesses the potential for the gall bladder's intraoperative imaging guidance needs to be further explored in vivo animal experiments. Herein, near‐infrared fluorophores ICG can display the specific uptake by the gall bladder cells and tissues. The dynamic process of biodistribution and the clearance of ICG in vivo in mice are clearly shown in real‐time live‐body imaging. Furthermore, ICG was rapidly excreted into the bile and lately biodistributed to the stomach after treatment in mice. Meanwhile, the signal‐to‐background ratio of the gall bladder demonstrated a tremendously higher level compared to other organs (stomach, heart, liver, lung, pancreas, spleen, intestine and duodenum). In conclusion, fluorescence navigation using ICG fluorescence imaging will provide good visualization and detection of the target lesions (gall bladder) in clinics such as diagnostic medical imaging and intraoperative navigation.

show abstract

“…In other words, enough hydrophobic residues are important for protein to come together with oil phase and interact with each other to form strong interfacial film ( 16 ), while the number of hydrophilic residues determines the molecular forces between protein-water and steric hindrance within droplets (derived from dangling hydrophilic groups) ( 17 ). Briefly, a more balanced polar and nonpolar group distribution is favored for interfacial adsorption and emulsion stabilization afterward, which is determined by the protein sequence ( 18 ). Xiong et al ( 19 ) verified the pea protein isolate subunits (i.e., isoforms of vicilins) well balanced with hydrophobic and hydrophilic groups displayed better interfacial activity.…”

Section: Emulsion Related Characteristics Of Proteinmentioning

confidence: 99%

Thoroughly review the recent progresses in improving O/W interfacial properties of proteins through various strategies

Zhang

Zhang²,

Chen³

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

Along with the future food market developing world widely, the personalized nutrition and rational function food design are found to be urgently attracted. Oil in a water (O/W) emulsion system has an excellent ability to maintain nutraceuticals and thus plays a promising role in producing future functional foods. Understanding the interfacial related mechanisms involved are essential for improving the quality of food products. Protein can effectively reduce interfacial tension and stable immiscible phases. The interfacial properties of proteins directly affect the emulsion qualities, which have gradually become a prospective topic. This review will first briefly discuss the interfacial-related fundamental factors of proteins. Next, the paper thoroughly overviewed current physical and chemical strategies tailored to improving the interfacial and emulsion properties of proteins. To be summarized, a higher flexibility could allow protein to be more easily unfolded and adsorbed onto the interface but could also possibly form a softer interfacial film. Several physical strategies, such as thermal, ultrasound and especially high-pressure homogenization are well applied to improve the interfacial properties. The interfacial behavior is also altered by various green chemical strategies, such as pH adjustment, covalent modification, and low molecular weight (LMW) surfactant addition. These strategies upgraded emulsion properties by increasing adsorption load, accelerating diffusion and adsorption rate, associated with lowering interfacial tension, and promoting interfacial protein interactions. Future researches targeted at elucidating interfacial-bulk protein interactions, unraveling interfacial behavior through in silico tools, exploring connection between interfacial-industrial processing properties, and clarifying the interfacial-sensory-digestive relationships of O/W emulsions is needed to develop emulsion applications.

show abstract

Applications of serum albumins in delivery systems: Differences in interfacial behaviour and interacting abilities with polysaccharides

Cited by 48 publications

References 148 publications

Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin

Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin

Indocyanine green for targeted imaging of the gall bladder and fluorescence navigation

Thoroughly review the recent progresses in improving O/W interfacial properties of proteins through various strategies

Contact Info

Product

Resources

About