Applications of <scp>NMR</scp> metabolomics to the study of foodstuffs: Truffle, kiwifruit, lettuce, and sea bass

Sobolev, Anatoly P.; Capitani, Donatella

doi:10.1002/elps.201100668

Cited by 44 publications

(32 citation statements)

References 99 publications

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“…NMR is widely used as an analytical technique in the field of metabolomics [1,2,3]. Often, it is applied as a non-targeted fingerprinting method, i.e.…”

Section: Introductionmentioning

confidence: 99%

Influence of Freezing and Storage Procedure on Human Urine Samples in NMR-Based Metabolomics

et al. 2013

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It is consensus in the metabolomics community that standardized protocols should be followed for sample handling, storage and analysis, as it is of utmost importance to maintain constant measurement conditions to identify subtle biological differences. The aim of this work, therefore, was to systematically investigate the influence of freezing procedures and storage temperatures and their effect on NMR spectra as a potentially disturbing aspect for NMR-based metabolomics studies. Urine samples were collected from two healthy volunteers, centrifuged and divided into aliquots. Urine aliquots were frozen either at −20 °C, on dry ice, at −80 °C or in liquid nitrogen and then stored at −20 °C, −80 °C or in liquid nitrogen vapor phase for 1–5 weeks before NMR analysis. Results show spectral changes depending on the freezing procedure, with samples frozen on dry ice showing the largest deviations. The effect was found to be based on pH differences, which were caused by variations in CO2 concentrations introduced by the freezing procedure. Thus, we recommend that urine samples should be frozen at −20 °C and transferred to lower storage temperatures within one week and that freezing procedures should be part of the publication protocol.

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“…NMR is widely used as an analytical technique in the field of metabolomics [1,2,3]. Often, it is applied as a non-targeted fingerprinting method, i.e.…”

Section: Introductionmentioning

confidence: 99%

Influence of Freezing and Storage Procedure on Human Urine Samples in NMR-Based Metabolomics

et al. 2013

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“…This type of analysis, also called “target analysis,” usually requires specific extractions and sample pretreatment. In contrast to this, NMR spectroscopy has gained an important role among the various analytical techniques used to analyze foodstuff owing to its “high-throughput” nature and high analytical precision [32]. Many applications have been reported in the literature showing the potential of an NMR-based approach to investigate the geographical origin, quality control, and processing of foodstuff [33,34,35].…”

Section: Resultsmentioning

confidence: 99%

FoodPro: A Web-Based Tool for Evaluating Covariance and Correlation NMR Spectra Associated with Food Processes

et al. 2016

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Foods from agriculture and fishery products are processed using various technologies. Molecular mixture analysis during food processing has the potential to help us understand the molecular mechanisms involved, thus enabling better cooking of the analyzed foods. To date, there has been no web-based tool focusing on accumulating Nuclear Magnetic Resonance (NMR) spectra from various types of food processing. Therefore, we have developed a novel web-based tool, FoodPro, that includes a food NMR spectrum database and computes covariance and correlation spectra to tasting and hardness. As a result, FoodPro has accumulated 236 aqueous (extracted in D2O) and 131 hydrophobic (extracted in CDCl3) experimental bench-top 60-MHz NMR spectra, 1753 tastings scored by volunteers, and 139 hardness measurements recorded by a penetrometer, all placed into a core database. The database content was roughly classified into fish and vegetable groups from the viewpoint of different spectrum patterns. FoodPro can query a user food NMR spectrum, search similar NMR spectra with a specified similarity threshold, and then compute estimated tasting and hardness, covariance, and correlation spectra to tasting and hardness. Querying fish spectra exemplified specific covariance spectra to tasting and hardness, giving positive covariance for tasting at 1.31 ppm for lactate and 3.47 ppm for glucose and a positive covariance for hardness at 3.26 ppm for trimethylamine N-oxide.

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“…136 154 e oriundos de plantas, 149,155 dentre outras. Dentro da ciência de alimentos, uma atenção mais detalhada é dada à pesquisa em nutrição, visando correlacionar dieta, estilo de vida e saúde, com genes, proteínas e metabólitos.…”

Section: Foodomics E Nutriçãounclassified

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Canuto¹,

Costa²,

Cruz³

et al. 2017

Quim. Nova

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METABOLOMICS: DEFINITIONS, STATE-OF-THE-ART AND REPRESENTATIVE APPLICATIONS.Metabolomics is an emerging and promising omics approach used to understand biological mechanisms. By untargeted and targeted metabolomics analyses, metabolites are determined in biological samples (fluids, cells, tissues, etc.) by comparison of control groups with altered groups, undergoing different therapies, submitted to differing stress levels, dietary modulation, or promoted by a disease, or specific condition, etc., using sophisticated analytical techniques, and advanced data treatment and statistical analyses. In this review, the concepts involved in metabolomics studies were presented, describing in details all steps involved in the metabolomics workflow, for untargeted and targeted strategies. Finally, the potential of metabolomics is illustrated by applications in representative areas: clinical, environmental, food and nutrition, forensic toxicology, microbiology, parasitology, plants, and sports. Relevant reviews were compiled to characterize each of these areas, and a corresponding application of untargeted and targeted metabolomics were described.Keywords: metabolomics; untargeted metabolomics; targeted metabolomics; metabolomics workflow. INTRODUÇÃOAs ciências ômicas buscam o entendimento do funcionamento celular dos organismos e suas alterações biológicas. Fazem parte deste conjunto de ciências, a genômica (estudo da alteração dos genes), a transcriptômica (estudo das alterações dos transcritos), a proteômica (estudo das alterações das proteínas), e a metabolômica (estudo das alterações dos metabólitos). Metabólitos são produtos intermediários ou finais do metabolismo em uma amostra biológica.1 O conjunto de todos os metabólitos de baixa massa molecular (até 1500 Da), presentes ou alterados em um sistema biológico, é chamado de metaboloma (do inglês, metabolome).2 A pesquisa relacionada a metabólitos vem sendo desenvolvida há décadas, mas em 1999, Nicholson et al. definiram a metabonômica (do inglês, metabonomics), como sendo a medida quantitativa da resposta metabólica de um sistema biológico após estímulos fisiopatológicos ou modificações genéticas.3 Já o termo metabolômica (do inglês, metabolomics) foi introduzido em 2001, por Oliver Fiehn, como sendo a análise abrangente e quantitativa do metaboloma de um sistema biológico. 4 Além disso, a literatura nos apresenta outras denominações envolvendo esse campo da ciência, tais como: perfil metabólico (do inglês, metabolic profiling), descrito como sendo a análise de metabólitos previamente selecionados de rotas bioquímicas específicas, 5 a impressão digital metabólica (do inglês, metabolic fingerprinting), que é definida como sendo "uma classificação de amostras de acordo com sua origem ou sua relevância biológica", 3 e, por último, análise footprinting (ainda sem tradução para o português), para se referir aos metabólitos excretados por uma célula em condições controladas. Observa-se, portanto, uma divergência em relação às terminologias aplicadas às análises metabolômic...

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Applications of NMR metabolomics to the study of foodstuffs: Truffle, kiwifruit, lettuce, and sea bass

Cited by 44 publications

References 99 publications

Influence of Freezing and Storage Procedure on Human Urine Samples in NMR-Based Metabolomics

Influence of Freezing and Storage Procedure on Human Urine Samples in NMR-Based Metabolomics

FoodPro: A Web-Based Tool for Evaluating Covariance and Correlation NMR Spectra Associated with Food Processes

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

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