Applications of restriction endonuclease fingerprinting of chromosomal DNA of Neisseria meningitidis

Bjorvatn, Bjarne; Lund, Vera; Kristiansen, Bjørn-Erik; Korsnes, L.; Spanne, Oddvar; Lindqvist, Björn H.

doi:10.1128/jcm.19.6.763-765.1984

Cited by 102 publications

(30 citation statements)

References 12 publications

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“…Genotyping DNA-fingerprints were used for genotyping of the different V. anguiilarum isolates according to techniques published by Bjorvatn, Lund, Kristiansen, Korsnes, Spanne & Lindquist (1984). Phenol/chloroform extracted DNA from the various strains were digested with a sequencespecific (restriction) endonuclease, Hindlll (BioLabs, England), and fragments were electrophoretically separated in a Wide Mini SubTM Cell, Bio-Rad, Sweden, on a 1% agarose gel (DNA grade agarose, Bio-Rad, Sweden).…”

Section: Serotypingmentioning

confidence: 99%

Vaccination experiments and studies of the humoral immune responses in cod, Gadus morhua L., to four strains of monoclonal‐defined Vibrio anguillarum

Espelid

Rødseth

Jørgensen

1991

Journal of Fish Diseases

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Abstract. Vibrio anguillarum isolated from diseased cod, Gadus morhua L., were serotyped by use of a panel of monoclonal antibodies. Four serotypes could be distinguished, having different lipopolysaccharide determinants. These phenotypic differences were also reflected in the genetic map, as revealed by fingerprinting of bacterial DNA. Antisera were raised in cod after immunization with the V. anguillarum serotypes, and Western blot techniques demonstrated production of specific antibodies mainly to LPS‐antigens. The immune system in cod discriminates to a eertain degree between the four serotypes as shown by crossreactions of the immune sera in elisa. Moreover, it was also shown that natural antibodies to bacterial antigens are present in non‐immune sera, but these specificities are non‐LPS in nature. As a consequence of the heterogeneity of the V. anguillarum strains, vaccination experiments were performed under laboratory conditions to compare the effectiveness of bacterins based on either single vaccines or polyvaccines. The results from these experiments were promising since challenge with one strain demonstrated 100% protection both in fish vaccinated with the homologous serotype as well as a mixture of all the four serotypes.

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Section: Serotypingmentioning

confidence: 99%

Vaccination experiments and studies of the humoral immune responses in cod, Gadus morhua L., to four strains of monoclonal‐defined Vibrio anguillarum

Espelid

Rødseth

Jørgensen

1991

Journal of Fish Diseases

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“…This REA method differentiates isolates according the pattern of a fraction of the DNA fragments obtained after hydrolysis with a restriction enzyme that have been separated by conventional polyacrylamide gel electrophoresis. Similar approaches for direct REA of total bacterial DNA have been previously employed to compare strains of several bacterial species (Bjorvatn et al ., 1984;Gerner-Smidt et al ., 1996;Djordjevic et al ., 1999), but these methods have not been generally adopted, probably because of the lack of a suitable protocol that could guarantee unambiguous and reproducible results. Our protocol solved this problem by using an improved combination of techniques to differentiate V. parahaemolyticus isolates.…”

Section: Introductionmentioning

confidence: 99%

Vibrio parahaemolyticus in shellfish and clinical samples during two large epidemics of diarrhoea in southern Chile

Fuenzalida

Hernández²,

Toro

et al. 2006

Environmental Microbiology

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Large epidemics of diarrhoea associated with seafood consumption and Vibrio parahaemolyticus occurred during the austral summers of 2004 and 2005 in the environs of Puerto Montt, Chile (41 degrees 29'S 72 degrees 24'W). There are no reports of V. parahaemolyticus infections before 2004 in this region, their absence being explained by the low ocean temperatures which seldom reach 16 degrees C. We analysed V. parahaemolyticus obtained from shellfish and clinical samples during epidemics. Isolates were examined using conventional protocols and an improved method for restriction enzyme analysis using total bacterial DNA which permits direct genome restriction enzyme analysis by conventional gel electrophoresis (DGREA) with a similar discrimination index as restriction fragment length polymorphism-pulsed field gel electrophoresis (RFLP-PFGE). Analysis of clinical samples showed that the epidemics were caused by the V. parahaemolyticus O3:K6 pandemic clonal group. On the other hand, analysis of shellfish samples during both epidemics showed that 53% contained V. parahaemolyticus (3-93 g(-1)). Detailed analysis of 50 positive shellfish samples showed that only three contained detectable levels of the pandemic clone. Most V. parahaemolyticus isolates obtained from shellfish corresponded to non-pandemic clones differentiated into 14 groups by DGREA. In summary, the causative agent during epidemics was only a minor component of a small but diverse population of V. parahaemolyticus in shellfish.

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“…Isolation of DNA was initially performed as described by Bjorvatn et al (14). with slight modifications (9).…”

Section: N a Isolationmentioning

confidence: 99%

“…5 4 . 2 kb on vertical 4% polyacrylamide gels as described before (14). Digests of 5 pg DNA were separated on horizontal 1% agarose (SeaKem GTG, FMC Bio Products, Rockland, ME) gels to study fragments within the range 1-12 kb.…”

Section: Restriction Frugment Length Polymorphism ( R F L P )mentioning

confidence: 99%

Emm gene polymorphism among temporally clustered group A streptococcal isolates in western Norway

Mylvaganam

Bjorvatn

Hofstad

et al. 2000

APMIS

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Nineteen group A streptococcal isolates obtained in western Norway from patients with invasive disease during a period of high morbidity and mortality were examined for clonality and emm gene polymorphism. These isolates belonged to the prevalent serotypes during the outbreak, namely T1, T3 or T6. Restriction fragment length polymorphism and sequencing of the emm genes were used to compare these isolates with 14 isolates of the same serotype but from non-invasive infections. The restriction analysis did not identify specific invasive clones. The emm genes in three of the four T3 isolates from invasive disease had nucleotide substitutions inducing a charge difference in the N-terminal part of the M protein. The 4 T6 isolates had a longer emm amplicon when compared to 15 isolates from superficial infections and also showed nucleotide substitutions that could induce conformational changes in the hypervariable end of the M protein. Restriction analysis of the emm amplicon of the T6 isolates in order to estimate the number of A- and C-repeats is described. The emm gene sequence served as an epidemiological marker within the serotypes T3 and T6, but the significance of the emm polymorphism displayed by the isolates from invasive disease is uncertain at this stage.

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Applications of restriction endonuclease fingerprinting of chromosomal DNA of Neisseria meningitidis

Cited by 102 publications

References 12 publications

Vaccination experiments and studies of the humoral immune responses in cod, Gadus morhua L., to four strains of monoclonal‐defined Vibrio anguillarum

Vaccination experiments and studies of the humoral immune responses in cod, Gadus morhua L., to four strains of monoclonal‐defined Vibrio anguillarum

Vibrio parahaemolyticus in shellfish and clinical samples during two large epidemics of diarrhoea in southern Chile

Emm gene polymorphism among temporally clustered group A streptococcal isolates in western Norway

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