Applications of recombinant proteins in aquaculture

Cell-based homologous expression system for in-vitro characterization of environmental effects on transmembrane peptide transport in fish

“…Furthermore, our demonstrated pipeline could be in use for other fish-originated cytosolic or transmembrane recombinant proteins. Most of the recombinant proteins used in fish research are translated and processed in heterologous expression systems, such as yeast, insects, and mammalian cell lines ( Mohammadzadeh et al, 2022 ). Since protein structure, and consequently its function, are greatly affected by the nature of the expression system, due to translational, post-translational, folding and membrane-integrations processes, our system could provide better conditions for recombinant protein expression.…”