Applications of Polydopamine-Modified Scaffolds in the Peripheral Nerve Tissue Engineering

Yan, Ji; Wu, Ruoyin; Liao, Su‐Lan; Jiang, Miao; Qian, Yun

doi:10.3389/fbioe.2020.590998

Cited by 51 publications

(38 citation statements)

References 66 publications

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“…The above results suggested that polydopamine could improve the cell adhesion and proliferation, which may be related to improved hydrophilicity and the abundant catechol-based adhesion groups in polydopamine. 54,55 However, we found a slight decrease in cell proliferation after loading AuNPs on the scaffold surfaces. Loading of AuNPs, a hydrophobic metal substance, could reduce the material surface hydrophilicity and the exposure of the catechol-based adhesive groups on the scaffold surface to some extent, which may affect the early adhesion and proliferation of the cells in a particular range.…”

Section: Cell Proliferation and Adhesionmentioning

confidence: 72%

Mussel-Inspired Gold Nanoparticle and PLGA/L-Lysine-g-Graphene Oxide Composite Scaffolds for Bone Defect Repair

Fu¹,

Jiang²,

Yang³

et al. 2021

IJN

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Purpose Insufficient biological activity heavily restricts the application and development of biodegradable bone implants. Functional modification of bone implants is critical to improve osseointegration and bone regeneration. Methods In this study, L-lysine functionalized graphene oxide (Lys-g-GO) nanoparticles and polydopamine-assisted gold nanoparticle (AuNPs-PDA) coatings were applied to improve the biological function of PLGA scaffold materials. The effects of Lys-g-GO nanoparticles and AuNPs-PDA functionalized coatings on the physicochemical properties of PLGA scaffolds were detected with scanning electron microscopy (SEM), contact angle measurement, and mechanical testing instruments. In vitro, the effects of composite scaffolds on MC3T3-E1 cell proliferation, adhesion, and osteogenic differentiation were studied. Finally, a radial defect model was used to assess the effect of composite scaffolds on bone defect healing. Results The prepared AuNPs-PDA@PLGA/Lys-g-GO composite scaffolds exhibited excellent mechanical strength, hydrophilicity and antibacterial properties. In vitro, this composite scaffold can significantly improve osteoblast adhesion, proliferation, osteogenic differentiation, calcium deposition, and other cell behaviour. In vivo, this composite scaffold can significantly promote the new bone formation and collagen deposition in the radial defect site and presented good biocompatibility. Conclusion The combination of bioactive nanoparticles and surface coatings shows considerable potential to enhance the osseointegration of bone implants.

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Section: Cell Proliferation and Adhesionmentioning

confidence: 72%

Mussel-Inspired Gold Nanoparticle and PLGA/L-Lysine-g-Graphene Oxide Composite Scaffolds for Bone Defect Repair

Fu¹,

Jiang²,

Yang³

et al. 2021

IJN

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“…The inhibition of excessive immune responses is of vital importance to restoring the balance in microenvironment after PNIs ( Qian et al, 2019b , 2020a , b ). The treatment of long-gap nerve defects requires highly functional biomimetic scaffolds to bridge the proximal and distal stumps ( Qian et al, 2019a , c ; Chen et al, 2020 ; Yan et al, 2020 ; Yang et al, 2020 ). In contrast, the treatment of severe nerve crush injuries relies more on the pharmacotherapy.…”

Section: Discussionmentioning

confidence: 99%

Tacrolimus-Induced Neurotrophic Differentiation of Adipose-Derived Stem Cells as Novel Therapeutic Method for Peripheral Nerve Injury

Yao

Yan²,

Li³

et al. 2021

Front. Cell. Neurosci.

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Peripheral nerve injuries (PNIs) are frequent traumatic injuries across the globe. Severe PNIs result in irreversible loss of axons and myelin sheaths and disability of motor and sensory function. Schwann cells can secrete neurotrophic factors and myelinate the injured axons to repair PNIs. However, Schwann cells are hard to harvest and expand in vitro, which limit their clinical use. Adipose-derived stem cells (ADSCs) are easily accessible and have the potential to acquire neurotrophic phenotype under the induction of an established protocol. It has been noticed that Tacrolimus/FK506 promotes peripheral nerve regeneration, despite the mechanism of its pro-neurogenic capacity remains undefined. Herein, we investigated the neurotrophic capacity of ADSCs under the stimulation of tacrolimus. ADSCs were cultured in the induction medium for 18 days to differentiate along the glial lineage and were subjected to FK506 stimulation for the last 3 days. We discovered that FK506 greatly enhanced the neurotrophic phenotype of ADSCs which potentiated the nerve regeneration in a crush injury model. This work explored the novel application of FK506 synergized with ADSCs and thus shed promising light on the treatment of severe PNIs.

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“…Artificial nerve scaffolds hold great promise for the biomedical treatment of injured nerves by connecting the damaged stumps and new nerve tissues form within the scaffold chamber ( Liu et al, 2019 ; Jiang et al, 2020 ; Yan et al, 2020 ; Li et al, 2021 ). However, the functional restoration of PNIs still remains unsatisfactory ( Qian et al, 2018b ; Qian et al, 2019b ; Qian et al, 2019c ).…”

Section: Final Remarksmentioning

confidence: 99%

Two-Dimensional Nanomaterials for Peripheral Nerve Engineering: Recent Advances and Potential Mechanisms

Yan

Chen

Rosso

et al. 2021

Front. Bioeng. Biotechnol.

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Peripheral nerve tissues possess the ability to regenerate within artificial nerve scaffolds, however, despite the advance of biomaterials that support nerve regeneration, the functional nerve recovery remains unsatisfactory. Importantly, the incorporation of two-dimensional nanomaterials has shown to significantly improve the therapeutic effect of conventional nerve scaffolds. In this review, we examine whether two-dimensional nanomaterials facilitate angiogenesis and thereby promote peripheral nerve regeneration. First, we summarize the major events occurring after peripheral nerve injury. Second, we discuss that the application of two-dimensional nanomaterials for peripheral nerve regeneration strategies by facilitating the formation of new vessels. Then, we analyze the mechanism that the newly-formed capillaries directionally and metabolically support neuronal regeneration. Finally, we prospect that the two-dimensional nanomaterials should be a potential solution to long range peripheral nerve defect. To further enhance the therapeutic effects of two-dimensional nanomaterial, strategies which help remedy the energy deficiency after peripheral nerve injury could be a viable solution.

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Applications of Polydopamine-Modified Scaffolds in the Peripheral Nerve Tissue Engineering

Cited by 51 publications

References 66 publications

Mussel-Inspired Gold Nanoparticle and PLGA/L-Lysine-g-Graphene Oxide Composite Scaffolds for Bone Defect Repair

Mussel-Inspired Gold Nanoparticle and PLGA/L-Lysine-g-Graphene Oxide Composite Scaffolds for Bone Defect Repair

Tacrolimus-Induced Neurotrophic Differentiation of Adipose-Derived Stem Cells as Novel Therapeutic Method for Peripheral Nerve Injury

Two-Dimensional Nanomaterials for Peripheral Nerve Engineering: Recent Advances and Potential Mechanisms

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