Applications of ovarian tissue transplantation in experimental biology and medicine

Torrents, E; Boiso, Irene; Barri, P. N.; Veiga, Anna

doi:10.1093/humupd/dmg036

Cited by 57 publications

(35 citation statements)

References 58 publications

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“…2 The follicle expressing Bax protein (▲) Fig. 3 The follicular cell expressing PCNA in graft (▲), the stromal cell expressing PCNA in graft(▼), the kidney tissue (★) transplantation after completed cancer treatment has been suggested as an alternative to restore fertility for girls and women who are at high risk for ovarian failure after chemotherapy or radiotherapy [9]. Earlier work revealed that more than 50% of primordial follicles are lost as a result of the frozen-thawed process and initial ischemia [10,11].…”

Section: Discussionmentioning

confidence: 99%

Is caspase inhibition a valid therapeutic strategy in cryopreservation of ovarian tissue?

Zhang

Yao

et al. 2009

J Assist Reprod Genet

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Purpose The aim of this study is to determine whether inclusion of caspase inhibitor can improve the efficacy of cryopreservation of ovarian tissue. Methods Mice were randomly assigned to the Group A (fresh control group) Group B (inclusion of caspase inhibitor) and Group C (non-inclusion of caspase inhibitor). Ovarian tissue in Group B and Group C was vitrifiedthawed. TUNEL assay and Bax protein detection were measured after cryopreservation. The mice in all groups received autotransplantation. The number of days before the resumption of estrous cycles was measured daily from the 5th day after surgery, and the percentage of cells expressing PCNA in grafts was measured one month following transplantation. Results The incidence of TUNEL positive follicles in Group B was significantly higher than that in Group C. Similarly, the percentage of follicles expressing Bax protein in Group B was significantly higher than that in Group C. The number of days before the resumption of estrous cycles in Group B was significantly less than that in Group C. In addition, the percentage of follicular and stromal cells expressing PCNA of grafts in Group B was significantly higher than that in Group C. ConclusionsThe global caspase inhibitor Z-VAD-FMK decreases the incidence of apoptosis of ovarian tissue induced by cryopreservation, and inclusion of caspase inhibitor improves the efficacy of cryopreservation of ovarian tissue.

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Section: Discussionmentioning

confidence: 99%

Is caspase inhibition a valid therapeutic strategy in cryopreservation of ovarian tissue?

Zhang

Yao

et al. 2009

J Assist Reprod Genet

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“…Intact and fresh ovary transplant have been successfully used in rats, rabbits, sheep, dogs and humans, independent of the implantation site. The loci most used have been the subcutaneous space, the renal capsule and the abdominal cavity, all subjected to temperature and pressure influence 12,13,14 . It is believed that the best place for ovary implantation is at close proximity to an important blood vessel like the renal or the femoral artery; accordingly, the subcutaneous site can be viewed to be the worst place in terms of blood supply to the follicle 4 .…”

Section: Discussionmentioning

confidence: 99%

Indirect evaluation of estrogenic activity post heterotopic ovarian autograft in rats

et al. 2008

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Purpose: To morphologically evaluate the estrogenic effect on the uterus and vagina of rats submitted to ovarian autografts. Methods: Twenty Wistar EPM-1 adult rats were bilaterally ovariectomized, followed by ovarian transplants in retroperitoneal regions. The animals were divided in four groups of five animals, according to the day of euthanasia: G4, G7, G14 and G21, corresponding to the 4th, 7th, 14th and 21st day after surgery, respectively. Vaginal smears were collected from the first day of surgery until euthanasia day. After that, the vagina and uterus were removed, fixed in 10% formaldehyde and submitted to histological analysis and stained with hematoxiline and eosine. Results: All animals showed estrous cycle changes during the experiment. In 4th day, the uterus showed low action of estrogen with small number of mitosis and eosinophils as well as poor development. On the 7th day, the endometrium was atrophic without mitotic signals and presented a small number of eosinophils. On the 14th and 21th days the histological findings were similar, with the presence of mitosis in the endometrial glands and intense leucocyte infiltration with a large number of eosinophils. Morphometric results showed that the endometrial and myometrial thickness as well as the number of eosinophils presented the highest values during the 14th and 21th days of the evaluation. The 7th day group also presented the lowest eosinophil numbers. Vaginal epithelium features were: 4th and 7th day groups presented non-keratinized stratified epithelium with 5 and 2 cell layers, respectively. The 14th and 21st day groups presented nonkeratinized stratified epithelium with 14 and 15 cell layers. Conclusion: Experimental ovarian autografts in the evaluated organs presented maximum estrogen activity after the 21st day of surgery, according to morphological and morphometric data. Key words: Transplantation. Ovary. Uterus. Vagina. Rats. RESUMOObjetivo: Avaliar morfologicamente a atividade estrogênica no útero e vagina de ratas submetidas a transplante autólogo de ovário. Métodos: Foram utilizadas 20 ratas adultas, Wistar EPM-1, submetidas à ooforectomia bilateral seguida de transplante de ovário em retroperitônio, distribuídas em grupos, conforme a data pré-estabelecida para eutanásia, com cinco animais cada: G4, G7, G14 e G21 -eutanásia no Indirect evaluation of estrogenic activity post heterotopic ovarian autograft in rats

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“…Despite these advantages, the technique of isolation primordial follicles remains difficult, 64 and it has not been possible to culture isolated human primordial follicles to the mature oocyte stage in vitro. 65 This method may exclude the possibility of reintroducing tumor cells into cancer patients by autografting of ovarian tissue.…”

Section: ) Isolated Primordial Folliclesmentioning

confidence: 99%

Fertility preservation for women with malignancies: current developments of cryopreservation

Chang

Suh

2008

J Gynecol Oncol

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The preservation of fertility in female cancer survivors has become an important health issue. The different cryopreservation options available for fertility preservation are embryo, oocyte, and ovarian tissue cryopreservation. Oocyte cryopreservation is available for women without partners, but there is a limited experience with this technique and the pregnancy rate is still low. In spite of recent reports of successful birth after autotransplantation of cryopreserved-thawed human ovarian cortical tissues, clinical experience is also limited and this technique remains still experimental. Whole ovary cryopreservation itself poses several challenges. Further researches for establishing optimal cryopreservation and thawing protocols and increasing post-thawing survival, pregnancy, and delivery rates are necessary. In this article, the strategies for fertility preservation in cancer survivors are discussed. The different options and their results are discussed, as well as their indications, efficacy and ethical issues.

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Applications of ovarian tissue transplantation in experimental biology and medicine

Cited by 57 publications

References 58 publications

Is caspase inhibition a valid therapeutic strategy in cryopreservation of ovarian tissue?

Is caspase inhibition a valid therapeutic strategy in cryopreservation of ovarian tissue?

Indirect evaluation of estrogenic activity post heterotopic ovarian autograft in rats

Fertility preservation for women with malignancies: current developments of cryopreservation

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