Applications of neutron and X-ray scattering to the study of biologically relevant model membranes

Pabst, Georg; Kučerka, Norbert; Nieh, Mu‐Ping; Rheinstädter, Maikel C.; Katsaras, John

doi:10.1016/j.chemphyslip.2010.03.010

Cited by 194 publications

(170 citation statements)

References 231 publications

(242 reference statements)

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“…Using this methodology and other related scattering techniques 18,25 values for the spontaneous curvatures of a number of biologically relevant lipids have been reported. Although these experimental approaches to determining c 0 are well established 26,27 , they involve lengthy experiments and require significant amounts of sample (typically > 100 mg). Consequently c 0 values have been documented for only a small number of lipids, mainly those that are most easily available, out of the rich diversity of lipid species that exist across the different domains of life.…”

Section: Introductionmentioning

confidence: 99%

Lipid Spontaneous Curvatures Estimated from Temperature-Dependent Changes in Inverse Hexagonal Phase Lattice Parameters: Effects of Metal Cations

et al. 2016

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Recently we reported a method for estimating the spontaneous curvatures of lipids from temperature dependent changes in the lattice parameter of inverse hexagonal liquid crystal phases of binary lipid mixtures. This method makes use of 1,2-dioleoyl-sn-glycerol-3-phosphoethanolamine, (DOPE) as a host lipid, which preferentially forms an inverse hexagonal phase, to which a guest lipid of unknown spontaneous curvature is added. The lattice parameters of these binary lipid mixtures are determined by small angle X-ray diffraction at a range of temperatures and the spontaneous curvature of the guest lipid is determined from these data. Here we report the use of this method on a wide range of lipids under different ionic conditions. We demonstrate that our method provides spontaneous curvature values for DOPE, cholesterol and monoolein that are within the range of values reported in the literature. Anionic lipids 1,2-dioleoyl-sn-glycerol-3-phosphatidic acid (DOPA) and 1,2-dioleoyl-sn-glycerol-3-phosphoserine (DOPS) were found to exhibit spontaneous curvatures that depend on the concentration of divalent cations present in the mixtures. We show that the range of curvatures estimated experimentally for DOPA and DOPS can be explained by a series of equilibria arising from lipid-cation exchange reactions. Our data indicate a universal relationship between the spontaneous curvature of a lipid and the extent to which it affects the lattice parameter of the hexagonal phase of DOPE when it is part of a binary mixture. This universal relationship affords a rapid way of estimating the spontaneous curvatures of lipids that are expensive, only available in small amounts or are of limited chemical stability.

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Section: Introductionmentioning

confidence: 99%

Lipid Spontaneous Curvatures Estimated from Temperature-Dependent Changes in Inverse Hexagonal Phase Lattice Parameters: Effects of Metal Cations

et al. 2016

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“…Due to this inherent disorderwhich is thought to be crucial for proper biological functionaveraged structural information is not easily obtained. 6 Fortunately, a renewed interest in the study of lipid−water systems has led to recent advances in neutron and X-ray scattering techniques, which are extensively used to elucidate the structure of biologically relevant membranes. 7 Access to such structural information is necessary in order to better understand the relationship between biomembrane structure and function.…”

Section: ■ Introductionmentioning

confidence: 99%

Molecular Structures of Fluid Phosphatidylethanolamine Bilayers Obtained from Simulation-to-Experiment Comparisons and Experimental Scattering Density Profiles

et al. 2014

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Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://doi.org/10.1021/jp511159qAccess and use of this website and the material on it are subject to the Terms and Conditions set forth at NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=9f16af25-9d91-47f7-b19b-387fe4c4022b http://nparc.cisti-icist.nrc-cnrc.gc.ca/fra/voir/objet/?id=9f16af25-9d91-47f7-b19b-387fe4c4022b ABSTRACT: Following our previous efforts in determining the structures of commonly used PC, PG, and PS bilayers, we continue our studies of fully hydrated, fluid phase PE bilayers. The newly designed parsing scheme for PE bilayers was based on extensive MD simulations, and is utilized in the SDP analysis of both X-ray and neutron (contrast varied) scattering measurements. Obtained experimental scattering form factors are directly compared to our simulation results, and can serve as a benchmark for future developed force fields. Among the evaluated structural parameters, namely, area per lipid A, overall bilayer thickness D B , and hydrocarbon region thickness 2D C , the PE bilayer response to changing temperature is similar to previously studied bilayers with different headgroups. On the other hand, the reduced hydration of PE headgroups, as well as the strong hydrogen bonding between PE headgroups, dramatically affects lateral packing within the bilayer. Despite sharing the same glycerol backbone, a markedly smaller area per lipid distinguishes PE from other bilayers (i.e., PC, PG, and PS) studied to date. Overall, our data are consistent with the notion that lipid headgroups govern bilayer packing, while hydrocarbon chains dominate the bilayer's response to temperature changes. ■ INTRODUCTIONMembranes that surround cells and separate their contents from the external environment are ubiquitous in nature. Biological membranes consist mainly of lipids and proteins, where it is widely accepted that the membrane's underlying structure is imparted by the lipid bilayer. As such, tremendous effort has been expe...

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“…Neutron and X-ray membrane diffraction of stacked aligned lipid bilayer phases has proved to be a powerful tool to understand bilayer structure [26][27][28][29][30][31][32][33] and probe the interactions between introduced molecules and bilayers [34][35][36][37][38]. This is accomplished by calculating scattering difference profiles that show the contribution to the unit cell profile of the introduced molecule.…”

Section: Introductionmentioning

confidence: 99%

Localization of trehalose in partially hydrated DOPC bilayers: insights into cryoprotective mechanisms

Kent

Hunt

Darwish

et al. 2014

J. R. Soc. Interface.

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Trehalose, a natural disaccharide with bioprotective properties, is widely recognized for its ability to preserve biological membranes during freezing and dehydration events. Despite debate over the molecular mechanisms by which this is achieved, and that different mechanisms imply quite different distributions of trehalose molecules with respect to the bilayer, there are no direct experimental data describing the location of trehalose within lipid bilayer membrane systems during dehydration. Here, we use neutron membrane diffraction to conclusively show that the trehalose distribution in a dioleoylphosphatidylcholine (DOPC) system follows a Gaussian profile centred in the water layer between bilayers. The absence of any preference for localizing near the lipid headgroups of the bilayers indicates that the bioprotective effects of trehalose at physiologically relevant concentrations are the result of non-specific mechanisms that do not rely on direct interactions with the lipid headgroups.

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Applications of neutron and X-ray scattering to the study of biologically relevant model membranes

Cited by 194 publications

References 231 publications

Lipid Spontaneous Curvatures Estimated from Temperature-Dependent Changes in Inverse Hexagonal Phase Lattice Parameters: Effects of Metal Cations

Lipid Spontaneous Curvatures Estimated from Temperature-Dependent Changes in Inverse Hexagonal Phase Lattice Parameters: Effects of Metal Cations

Molecular Structures of Fluid Phosphatidylethanolamine Bilayers Obtained from Simulation-to-Experiment Comparisons and Experimental Scattering Density Profiles

Localization of trehalose in partially hydrated DOPC bilayers: insights into cryoprotective mechanisms

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