Applications of Network Pharmacology in Traditional Chinese Medicine Research

Zhou, Zhuchen; Chen, Bing; Chen, Simiao; Lin, Minqiu; Ying, Chen; Jin, Shan; Chen, Weiyan; Zhang, Yuyan

doi:10.1155/2020/1646905

Cited by 193 publications

(134 citation statements)

References 50 publications

(47 reference statements)

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“…Fortunately, network pharmacology can be used to identify potential mechanisms of traditional Chinese medicine. 14 , 15 Thus, based on network pharmacology, this study was designed to explore whether gallincin protects against experimental UC by inhibiting inflammation and mucosal damage.…”

Section: Introductionmentioning

confidence: 99%

Gallincin ameliorates colitis-associated inflammation and barrier function in mice based on network pharmacology prediction

et al. 2020

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Objective To explore potential mechanisms and effects of gallincin on a mouse model of colitis induced by dextran sulfate sodium (DSS). Methods Network pharmacology analysis was used to predict the molecular mechanism of action of gallincin for treatment of colitis. Gallincin was administered orally to mice with DSS-induced colitis. Expression of tumor necrosis factor α (TNF-α), D-lactate, and interleukin-1β (IL-1β) and myeloperoxidase activity were assessed with real-time quantitative PCR and an enzyme-linked immunoassay, respectively. Expression of occludin, zonula occludens 1 (ZO-1), and phosphorylated extracellular signal-regulated protein kinase1/2 (p-ERK1/2) was analyzed with immunohistochemical staining and/or western blot assays. Results Using a network pharmacology approach, 12 mapping targets between gallincin and colitis were obtained, including ERK/mitogen-activated protein kinase. Further investigations in an experimental colitis mouse model showed that gallincin significantly ameliorated experimental colitis, reduced D-lactate levels, and remarkably increased occludin and ZO-1 expression, possibly in part by decreasing IL-1β, TNF-α, and p-ERK1/2 levels and inhibiting leukocyte penetration. Conclusions Gallincin regulated colonic barrier function and reduced colitis-associated inflammation, suggesting it is a promising drug for the treatment of ulcerative colitis.

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Section: Introductionmentioning

confidence: 99%

Gallincin ameliorates colitis-associated inflammation and barrier function in mice based on network pharmacology prediction

et al. 2020

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“…Systematic pharmacology is still in the early stage of development. At present, the data on drugs, compounds, proteins, and genes in the TCM database are incomplete, and the availability of the data obtained is limited [ 44 ]. In addition, there are differences between rat chondrocytes and human chondrocytes, and the present results need to be further verified in human chondrocytes and by in vivo experiments.…”

Section: Discussionmentioning

confidence: 99%

A Systematic Pharmacology and In Vitro Study to Identify the Role of the Active Compounds of Achyranthes bidentata in the Treatment of Osteoarthritis

Chen

Zheng

2020

Med Sci Monit

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Backgrounds Achyranthes bidentata is a Chinese traditional herbal medicine widely used to treat osteoarthritis (OA) . This study aimed to identify active compounds from Achyranthes bidentata through systematic pharmacology and in vitro experiments to find the targets of Achyranthes bidentata in the treatment of OA. Material/Methods We screened the active compounds of Achyranthes bidentata from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. Then, we used STITCH and Open Targets Platform databases to screen the active components and predict the potential targets of Achyranthes bidentata in the treatment of OA. Subsequently, we studied the compound-target network and protein interaction network and analyzed the enrichment of potential target proteins. Finally, we used Western blot analysis to verify the therapeutic effect of Achyranthes bidentata extract on the expression of OA-related target proteins. Results There were 7 active components in Achyranthes bidentata , which were strongly related to the 74 targets of OA. Quercetin, baicalein, and berberine are the critical active compounds of Achyranthes bidentata in the treatment of OA. Protein interaction analysis and in vitro experiments suggested that TNF, IL-6, and TP53 are the critical targets of Achyranthes bidentata in the treatment of OA. Functional enrichment analysis showed that Achyranthes bidentata plays a pharmacological role in OA through apoptosis, inflammation, and immune regulation. Conclusions Quercetin, baicalein, and berberine are the critical active compounds of Achyranthes bidentata in the treatment of OA. TNF, IL-6, and TP53 may be potential targets for the treatment of OA.

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“…[13] Currently, there is growing evidence about the reliability of the network pharmacology method, which may be an effective way to study the pharmacological mechanisms of TCM prescriptions. [11][12][13][14] In this study, network pharmacology analysis was used to visualize and decipher the complex relationships among the key compounds, targets, main biological processes and pathways, and disease. Moreover, molecular docking experiment was used to validate the binding a nity between key targets and their corresponding active compounds.…”

Section: Introductionmentioning

confidence: 99%

Potential Mechanism of Guizhi Decoction in Hypertension Treatment Based on Network Pharmacology and Dahl Salt-Sensitive Rat Model

Chen

Zhang

Wang

et al. 2020

Preprint

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Background Guizhi decoction (GZD), a classical Chinese herbal formula, has been widely used to treat hypertension, but its underlying mechanisms remain elusive. The present study aimed to explore its therapeutic effects and potential mechanisms in the treatment of hypertension using network pharmacology and experimental validation. Methods The active ingredients and corresponding targets were collected from Traditional Chinese Medicine Systems Pharmacology database and Analysis Platform (TCMSP). The targets related to hypertension were identified from multiple databases, and multiple networks were constructed to identify key compounds, hub targets, and main biological processes and pathways of GZD against hypertension. The Surflex-Dock software was used to validate the binding affinity between key targets and their corresponding active compounds. The Dahl salt-sensitive rat model was used to evaluate the therapeutic effects of GZD on hypertension. Results A total of 112 active ingredients, 222 targets of GZD and 341 hypertension- related targets were obtained. Furthermore, 56 overlapping targets were identified, five of which were determined as the hub targets to perform experimental verification, including interleukin 6 (IL-6), C-C motif chemokine 2 (CCL2), IL-1β, matrix metalloproteinase 2(MMP-2), and MMP9. Pathway enrichment

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Applications of Network Pharmacology in Traditional Chinese Medicine Research

Cited by 193 publications

References 50 publications

Gallincin ameliorates colitis-associated inflammation and barrier function in mice based on network pharmacology prediction

Gallincin ameliorates colitis-associated inflammation and barrier function in mice based on network pharmacology prediction

A Systematic Pharmacology and In Vitro Study to Identify the Role of the Active Compounds of Achyranthes bidentata in the Treatment of Osteoarthritis

Potential Mechanism of Guizhi Decoction in Hypertension Treatment Based on Network Pharmacology and Dahl Salt-Sensitive Rat Model

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