Applications of Nanometals in Cutaneous Infections

Nakazato, Gerson; Lonni, Audrey Alesandra Stinghen Garcia; Panagio, Luciano Aparecido; Camargo, Larissa Ciappina de; Gonçalves, Marcelly Chue; Reis, Guilherme Fonseca; Miranda-Sapla, Milena Menegazzo; Tomiotto-Pellissier, Fernanda; Kobayashi, Renata Katsuko Takayama

doi:10.1007/978-3-030-35147-2_4

Cited by 6 publications

(8 citation statements)

References 104 publications

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“…It is suggested that oxidative stress caused by AgNPs through increase in radicals production leads to damage of the nucleic acids and proteins, and consequently inhibition of proliferative processes in bacterial cells. 55 Researchers confirmed that AgNPs produced different toxic radicals including hydrogen peroxide (H 2 O 2 ) and hydroxyl radicals (OH). 61,62 Moreover, results of physical interaction investigation also pointed out that the bacterial cells were killed more effectively by "CS-AgNPs-antibiotic complexes", which is in consistent with previous study.…”

Section: Dovepressmentioning

confidence: 99%

“…53,54 Biogenic silver nanoparticles reported showing greater antibiofilm activity against these superbugs. 55 Nanocomposites of silver with other metals observed the disruption of biofilm structure and penetration of metal ions into under layers of bacterial colony. 56 Chitosan conjugated nanocomposites have profound antibacterial activity, due to the presence of an ammonia group in chitosan.…”

Section: Mechanism Of Synergistic Effectsmentioning

confidence: 99%

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<p>Synergistic Nanocomposites of Different Antibiotics Coupled with Green Synthesized Chitosan-Based Silver Nanoparticles: Characterization, Antibacterial, in vivo Toxicological and Biodistribution Studies</p>

Asghar

Yousuf

Shoaib

et al. 2020

IJN

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The present study reports chitosan functionalized green synthesized CS-AgNPs, conjugated with amoxicillin (AMX), cefixime (CEF), and levofloxacin (LVX) for safe and enhanced antibacterial activity. Methods: The CS-AgNPs and conjugates CS-AgNPs+AMX CS-AgNPs+CEF, and CS-AgNPs+LVX were characterized by UV-Vis, FTIR, SEM, TEM, EDX spectroscopy. The size distribution and zeta potential were measured using the dynamic light scattering (DLS) technique. The interaction between CS-AgNPs and antibiotic molecules was also investigated using UV-Vis spectroscopy at the concentrations of 5, 50, 500, and 5000 µM for each antibiotic. Antibacterial activity and synergism were assessed by the Fractional Inhibitory Concentration (FIC) index. The mechanism for synergistic activity was investigated by the detection of hydroxyl species based on the chemiluminescence of luminol. The biocompatibility index (BI) was calculated from IC 50 using the HeLa cell line. In vivo toxicity and tissue distribution of silver ions were evaluated on Sprague Dawley rats. Physical interactions of antibiotics and significant (P<0.05) antibacterial activity were observed after loading on CS-AgNPs surfaces. Results: The spherical shape nanocomposites of CS-AgNPs with different antibiotics were prepared with mean size ranges of 80-120 nm. IC 50 of antibiotics-conjugated CS-AgNPs decreased compared to CS-AgNPs. The biocompatibility (BI) index showed that antibioticsconjugated CS-AgNPs have high antibacterial potential and low toxicity. Highly significant (P<0.005) increase in the generation of hydroxyl species indicated the radical scavenging mechanism for synergistic activity of CS-AgNPs after combined with different antibiotics. Biochemical analysis and histopathological examinations confirmed low toxicity with minor hepatotoxicity at higher doses. After oral administration, extensive distribution of Ag ion was observed in spleen and liver. Conclusion: The study demonstrates positive attributes of antibiotics-conjugated CS-AgNPs, as a promising antibacterial agent with low toxicity.

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Section: Dovepressmentioning

confidence: 99%

Section: Mechanism Of Synergistic Effectsmentioning

confidence: 99%

<p>Synergistic Nanocomposites of Different Antibiotics Coupled with Green Synthesized Chitosan-Based Silver Nanoparticles: Characterization, Antibacterial, in vivo Toxicological and Biodistribution Studies</p>

Asghar

Yousuf

Shoaib

et al. 2020

IJN

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“…Silver nanoparticles have large surface area and high oxidation-reduction potential, which provide them great effect against bacteria, including multidrug-resistant strains (Nowack et al, 2011;Cardozo et al, 2013;Biasi-Garbin et al, 2015;Ebrahiminezhad et al, 2016;Scandorieiro et al, 2016;Calderón-Jiménez et al, 2017;Sim et al, 2018;Simbine et al, 2019;Dalir et al, 2020). Because of their antimicrobial property, silver nanoparticles have been widely explored by the nanotechnology industry, since they, among other applications, are incorporated in the formulation of surface cleaners, textiles, toys, air and water disinfectants, antimicrobial inks, food packaging, wound dressing and other materials for cutaneous infections, coating for bone implants, dental prostheses, and catheters (Calderón-Jiménez et al, 2017;Nakazato et al, 2017Nakazato et al, , 2020Kobayshi et al, 2019). Other physicochemical properties of silver nanoparticles (high electrical and thermal conductivity, catalytic activity, and non-linear optical properties) allow for the development of new products and scientific applications (Calderón-Jiménez et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Biogenic Silver Nanoparticles Strategically Combined With Origanum vulgare Derivatives: Antibacterial Mechanism of Action and Effect on Multidrug-Resistant Strains

et al. 2022

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Multidrug-resistant bacteria have become a public health problem worldwide, reducing treatment options against several pathogens. If we do not act against this problem, it is estimated that by 2050 superbugs will kill more people than the current COVID-19 pandemic. Among solutions to combat antibacterial resistance, there is increasing demand for new antimicrobials. The antibacterial activity of binary combinations containing bioAgNP (biogenically synthesized silver nanoparticles using Fusarium oxysporum), oregano essential oil (OEO), carvacrol (Car), and thymol (Thy) was evaluated: OEO plus bioAgNP, Car plus bioAgNP, Thy plus bioAgNP, and Car plus Thy. This study shows that the mechanism of action of Thy, bioAgNP, and Thy plus bioAgNP involves damaging the membrane and cell wall (surface blebbing and disruption seen with an electron microscope), causing cytoplasmic molecule leakage (ATP, DNA, RNA, and total proteins) and oxidative stress by enhancing intracellular reactive oxygen species and lipid peroxidation; a similar mechanism happens for OEO and Car, except for oxidative stress. The combination containing bioAgNP and oregano derivatives, especially thymol, shows strategic antibacterial mechanism; thymol disturbs the selective permeability of the cell membrane and consequently facilitates access of the nanoparticles to bacterial cytoplasm. BioAgNP-treated Escherichia coli developed resistance to nanosilver after 12 days of daily exposition. The combination of Thy and bioAgNP prevented the emergence of resistance to both antimicrobials; therefore, mixture of antimicrobials is a strategy to extend their life. For antimicrobials alone, minimal bactericidal concentration ranges were 0.3–2.38 mg/ml (OEO), 0.31–1.22 mg/ml (Car), 0.25–1 mg/ml (Thy), and 15.75–31.5 μg/ml (bioAgNP). The time-kill assays showed that the oregano derivatives acted very fast (at least 10 s), while the bioAgNP took at least 30 min to kill Gram-negative bacteria and 7 h to kill methicillin-resistant Staphylococcus aureus (MRSA). All the combinations resulted in additive antibacterial effect, reducing significantly minimal inhibitory concentration and acting faster than the bioAgNP alone; they also showed no cytotoxicity. This study describes for the first time the effect of Car and Thy combined with bioAgNP (produced with F. oxysporum components) against bacteria for which efficient antimicrobials are urgently needed, such as carbapenem-resistant strains (E. coli, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa) and MRSA.

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“…Ancient civilizations such as the Persians, Phoenicians, Greeks, Romans, and Egyptians have made use of silver antimicrobial properties to preserve food and water and treat eye infections and wounds [ 34 , 35 ]. Nanotechnology has allowed reviving the use of this metal as an antimicrobial since silver nanoparticles (AgNP) have been used for over 100 years [ 36 ] and are currently incorporated into various products and processes of our routine (such as formulation of surface cleaners, water disinfection processes, wound dressing, several materials for cutaneous infections, coating for bone implants, and catheters) [ 37 , 38 , 39 ]. The antibiofilm activity of AgNP has been shown by several studies [ 40 , 41 , 42 ] since silver has high oxidation-reduction potential, and the large surface area of its nanoparticle form provides them broad antimicrobial properties [ 36 , 43 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm Effect of Biogenic Silver Nanoparticles Combined with Oregano Derivatives against Carbapenem-Resistant Klebsiella pneumoniae

et al. 2023

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Resistant bacteria may kill more people than COVID-19, so the development of new antibacterials is essential, especially against microbial biofilms that are reservoirs of resistant cells. Silver nanoparticles (bioAgNP), biogenically synthesized using Fusarium oxysporum, combined with oregano derivatives, present a strategic antibacterial mechanism and prevent the emergence of resistance against planktonic microorganisms. Antibiofilm activity of four binary combinations was tested against enteroaggregative Escherichia coli (EAEC) and Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC): oregano essential oil (OEO) plus bioAgNP, carvacrol (Car) plus bioAgNP, thymol (Thy) plus bioAgNP, and Car plus Thy. The antibiofilm effect was accessed using crystal violet, MTT, scanning electron microscopy, and Chromobacterium violaceum anti-quorum-sensing assays. All binary combinations acted against preformed biofilm and prevented its formation; they showed improved antibiofilm activity compared to antimicrobials individually by reducing sessile minimal inhibitory concentration up to 87.5% or further decreasing biofilm metabolic activity and total biomass. Thy plus bioAgNP extensively inhibited the growth of biofilm in polystyrene and glass surfaces, disrupted three-dimensional biofilm structure, and quorum-sensing inhibition may be involved in its antibiofilm activity. For the first time, it is shown that bioAgNP combined with oregano has antibiofilm effect against bacteria for which antimicrobials are urgently needed, such as KPC.

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Applications of Nanometals in Cutaneous Infections

Cited by 6 publications

References 104 publications

<p>Synergistic Nanocomposites of Different Antibiotics Coupled with Green Synthesized Chitosan-Based Silver Nanoparticles: Characterization, Antibacterial, in vivo Toxicological and Biodistribution Studies</p>

<p>Synergistic Nanocomposites of Different Antibiotics Coupled with Green Synthesized Chitosan-Based Silver Nanoparticles: Characterization, Antibacterial, in vivo Toxicological and Biodistribution Studies</p>

Biogenic Silver Nanoparticles Strategically Combined With Origanum vulgare Derivatives: Antibacterial Mechanism of Action and Effect on Multidrug-Resistant Strains

Antibiofilm Effect of Biogenic Silver Nanoparticles Combined with Oregano Derivatives against Carbapenem-Resistant Klebsiella pneumoniae

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